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FASTK (NM_006712) Human Tagged ORF Clone
CAT＃: RC204063
FASTK (Myc-DDK-tagged)-Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1
ORF Plasmid: tGFP
"NM_006712" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FAST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204063 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGGCCGCGGGGGGAACCCGGCCCCCGGGCCCCGAGACCGACTGAGGGAGCGACCTGCGCAGGGC CCGGGGAGTCATGGTCTCCATCACCCAACTCCATGCTTCGAGTCCTGCTCTCTGCTCAGACCTCCCCTGC TCGGCTGTCTGGCCTGCTGCTGATCCCTCCAGTACAGCCCTGCTGTTTGGGGCCCAGCAAATGGGGGGAC CGGCCTGTTGGAGGAGGCCCCAGTGCAGGTCCTGTGCAAGGACTGCAGCGGCTTCTGGAACAGGCGAAGA GCCCTGGGGAGCTGCTGCGCTGGCTGGGCCAGAACCCCAGCAAGGTGCGCGCCCACCACTACTCGGTGGC GCTTCGTCGTCTGGGCCAGCTCTTGGGGTCTCGGCCACGGCCCCCTCCTGTGGAGCAGGTCACACTGCAG GACTTGAGTCAGCTCATCATCCGAAACTGCCCCTCCTTTGACATTCACACCATCCACGTGTGTCTGCACC TTGCAGTCTTACTTGGCTTTCCATCTGATGGTCCCCTGGTGTGTGCCCTGGAACAGGAGCGAAGGCTCCG CCTCCCTCCGAAGCCACCTCCCCCTTTGCAGCCCCTTCTCCGAGGTGGGCAAGGGTTGGAAGCTGCTCTA AGCTGCCCCCGTTTTCTGCGGTATCCACGGCAGCATCTGATCAGCAGCCTGGCAGAGGCAAGGCCAGAGG AACTGACTCCCCACGTGATGGTGCTCCTGGCCCAGCACCTGGCCCGGCACCGGTTGCGGGAGCCCCAGCT TCTGGAAGCCATTGCCCACTTCCTGGTGGTTCAGGAAACGCAACTCAGCAGCAAGGTGGTACAGAAGTTG GTCCTGCCCTTTGGGCGACTGAACTACCTGCCCCTGGAACAGCAGTTTATGCCCTGCCTTGAGAGGATCC TGGCTCGGGAAGCAGGGGTGGCACCCCTGGCTACAGTCAACATCTTGATGTCACTGTGCCAACTGCGGTG CCTGCCCTTCAGAGCCCTGCACTTTGTTTTTTCCCCTGGCTTCATCAACTACATCAGTGGCACCCCTCAT GCTCTGATTGTGCGTCGCTACCTCTCCCTGCTGGACACGGCCGTGGAGCTGGAGCTCCCAGGATACCGGG GTCCCCGCCTTCCCCGAAGGCAGCAAGTGCCCATCTTTCCCCAGCCTCTCATCACCGACCGTGCCCGCTG CAAGTACAGTCACAAGGACATAGTAGCTGAGGGGTTGCGCCAGCTGCTGGGGGAGGAGAAATACCGCCAG GACCTGACTGTGCCTCCAGGCTACTGCACAGACTTCCTGCTGTGCGCCAGCAGCTCTGGTGCTGTGCTTC CCGTGAGGACCCAGGACCCCTTCCTGCCATACCCACCAAGGTCCTGCCCACAGGGCCAGGCTGCCTCTAG CGCCACTACTCGAGACCCTGCCCAGAGGGTGGTGCTGGTGTTGCGGGAACGCTGGCATTTCTGCCGGGAC GGCCGGGTGCTGCTGGGCTCGAGGGCCCTGAGGGAGCGGCACCTAGGCCTGATGGGCTACCAGCTCCTGC CGCTACCCTTCGAGGAACTGGAGTCCCAGAGAGGCCTGCCCCAGCTCAAGAGCTACCTGAGGCAGAAGCT CCAGGCCCTGGGCCTGCGCTGGGGGCCTGAAGGGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204063 protein sequence
Red=Cloning site Green=Tags(s) MRRPRGEPGPRAPRPTEGATCAGPGESWSPSPNSMLRVLLSAQTSPARLSGLLLIPPVQPCCLGPSKWGD RPVGGGPSAGPVQGLQRLLEQAKSPGELLRWLGQNPSKVRAHHYSVALRRLGQLLGSRPRPPPVEQVTLQ DLSQLIIRNCPSFDIHTIHVCLHLAVLLGFPSDGPLVCALEQERRLRLPPKPPPPLQPLLRGGQGLEAAL SCPRFLRYPRQHLISSLAEARPEELTPHVMVLLAQHLARHRLREPQLLEAIAHFLVVQETQLSSKVVQKL VLPFGRLNYLPLEQQFMPCLERILAREAGVAPLATVNILMSLCQLRCLPFRALHFVFSPGFINYISGTPH ALIVRRYLSLLDTAVELELPGYRGPRLPRRQQVPIFPQPLITDRARCKYSHKDIVAEGLRQLLGEEKYRQ DLTVPPGYCTDFLLCASSSGAVLPVRTQDPFLPYPPRSCPQGQAASSATTRDPAQRVVLVLRERWHFCRD GRVLLGSRALRERHLGLMGYQLLPLPFEELESQRGLPQLKSYLRQKLQALGLRWGPEGG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006712 |
| ORF Size | 1647 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006712.5 |
| RefSeq Size | 1885 bp |
| RefSeq ORF | 1650 bp |
| Locus ID | 10922 |
| UniProt ID | Q14296 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 61.1 kDa |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204063L1 | Lenti ORF clone of Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204063L2 | Lenti ORF clone of Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204063L3 | Lenti ORF clone of Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204063L4 | Lenti ORF clone of Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204063 | FASTK (tGFP-tagged) - Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1 |
CNY 4,370.00 |
|
| SC115926 | FASTK (untagged)-Human Fas-activated serine/threonine kinase (FASTK), transcript variant 1 |
CNY 2,950.00 |
