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OXCT1 (NM_000436) Human Tagged ORF Clone
CAT＃: RC203764
- TrueORF®
OXCT1 (Myc-DDK-tagged)-Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_000436" in other vectors (6)
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CNY 3,872.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OXCT; SCOT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203764 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTCTCAAACTCCTCTCCTCCGGGCTTCGGCTCTGCGCCTCTGCCCGCGGATCTGGGGCAACCT GGTACAAGGGATGTGTTTGTTCCTTTTCCACCAGTGCTCATCGCCATACCAAGTTTTATACAGATCCAGT AGAAGCTGTAAAAGACATCCCTGATGGTGCCACGGTTTTGGTTGGTGGTTTTGGGCTATGTGGAATTCCA GAGAATCTTATAGATGCTTTACTGAAAACTGGAGTAAAAGGACTAACTGCAGTCAGCAACAATGCAGGGG TTGACAATTTTGGTTTGGGGCTTTTGCTTCGGTCCAAGCAGATAAAACGCATGGTCTCTTCATATGTGGG AGAAAATGCAGAATTTGAACGACAGTACTTATCTGGTGAATTAGAAGTGGAGCTGACACCACAGGGCACA CTTGCAGAGAGGATCCGTGCAGGCGGGGCTGGAGTTCCTGCATTTTACACCCCAACAGGGTATGGGACCC TGGTACAAGAAGGAGGATCGCCCATCAAATACAACAAAGATGGCAGTGTTGCCATTGCCAGTAAGCCAAG AGAGGTGAGGGAGTTCAATGGTCAGCACTTTATTTTGGAGGAAGCAATTACAGGGGATTTTGCTTTGGTG AAAGCCTGGAAGGCGGACCGAGCAGGAAACGTGATTTTCAGGAAAAGTGCAAGGAATTTCAACTTGCCAA TGTGCAAAGCTGCAGAAACCACAGTGGTAGAGGTTGAAGAAATTGTGGATATTGGAGCATTTGCTCCAGA AGACATCCATATTCCTCAGATTTATGTACATCGCCTTATAAAGGGAGAAAAATATGAGAAAAGAATTGAG CGTTTATCAATCCGGAAAGAGGGAGATGGGGAAGCCAAATCTGCTAAACCTGGAGATGACGTAAGGGAAC GAATCATCAAGAGGGCCGCTCTTGAGTTTGAGGATGGCATGTATGCTAATTTGGGCATAGGAATCCCTCT CCTGGCCAGCAATTTTATCAGCCCAAATATAACTGTTCATCTTCAAAGTGAAAATGGAGTTCTGGGTTTG GGTCCATATCCACGACAACATGAAGCTGATGCAGATCTCATCAATGCAGGCAAGGAAACAGTTACTATTC TTCCAGGAGCCTCTTTTTTCTCCAGCGATGAATCATTTGCAATGATTAGAGGTGGACACGTCGATCTGAC AATGCTAGGAGCGATGCAGGTTTCCAAATATGGTGACCTGGCTAACTGGATGATACCTGGGAAGATGGTG AAAGGAATGGGAGGTGCTATGGATTTAGTGTCCAGTGCGAAAACCAAAGTGGTGGTCACCATGGAGCATT CTGCAAAGGGAAATGCACATAAAATCATGGAGAAATGTACATTACCATTGACTGGAAAGCAATGTGTCAA CCGCATTATTACTGAAAAGGCTGTGTTTGATGTGGACAAGAAGAAAGGGTTGACTCTGATTGAGCTCTGG GAAGGCCTGACAGTGGATGACGTACAGAAGAGTACTGGGTGTGATTTTGCAGTTTCACCAAAACTCATGC CAATGCAGCAGATCGCAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203764 protein sequence
Red=Cloning site Green=Tags(s) MAALKLLSSGLRLCASARGSGATWYKGCVCSFSTSAHRHTKFYTDPVEAVKDIPDGATVLVGGFGLCGIP ENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGT LAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALV KAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIE RLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGL GPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMV KGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELW EGLTVDDVQKSTGCDFAVSPKLMPMQQIAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000436 |
| ORF Size | 1560 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000436.4 |
| RefSeq Size | 3572 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 5019 |
| UniProt ID | P55809 |
| Domains | CoA_trans |
| Protein Pathways | Butanoate metabolism, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation |
| MW | 56.2 kDa |
| Gene Summary | This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203764L1 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203764L2 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC203764L3 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203764L4 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG203764 | OXCT1 (tGFP-tagged) - Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC119890 | OXCT1 (untagged)-Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein |
CNY 2,950.00 |
