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CARS2 (NM_024537) Human Tagged ORF Clone
CAT＃: RC203400
- TrueORF®
CARS2 (Myc-DDK-tagged)-Human cysteinyl-tRNA synthetase 2, mitochondrial (putative) (CARS2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_024537" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | COXPD27; cysRS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203400 representing NM_024537
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAGGACTACGCGCGGCCCAGGCCTGGGCCCCCCGCTGCTCCAGGCCGCGCTGGGCCTTGGGCGGG CTGGGTGGCACTGGCCTGCGGGCCGGGCGGCGAGCGGGGGGCGCGGGCGGGCCTGGCTGCAGCCCACGGG CCGGGAGACGGGTGTGCAGGTGTACAACAGCCTCACCGGGAGGAAGGAACCCCTAATCGTGGCGCACGCC GAAGCCGCCTCCTGGTATAGCTGTGGACCAACTGTATATGATCATGCGCACCTTGGCCATGCTTGCTCAT ATGTTAGATTTGATATCATTCGAAGGATCCTAACCAAGGTTTTTGGATGCAGCATAGTCATGGTGATGGG TATTACAGATGTAGATGATAAAATCATCAAAAGAGCCAATGAGATGAATATTTCCCCCGCTTCCCTCGCC AGTCTTTATGAGGAAGACTTCAAGCAGGACATGGCAGCCCTGAAGGTTCTCCCACCCACGGTGTACCTGA GGGTAACCGAAAATATTCCTCAGATAATTTCTTTCATTGAAGGAATCATTGCTCGTGGGAACGCTTATTC AACGGCAAAAGGCAATGTCTACTTCGATCTGAAGTCTAGAGGAGACAAGTATGGCAAATTGGTCGGCGTG GTCCCTGGTCCAGTCGGAGAGCCAGCGGACTCTGACAAGCGTCATGCCAGTGACTTCGCCCTGTGGAAGG CGGCCAAACCCCAGGAGGTGTTCTGGGCCTCTCCCTGGGGACCCGGGAGGCCGGGCTGGCACATCGAGTG CTCTGCCATCGCTAGTATGGTATTTGGAAGTCAACTGGATATCCATTCAGGTGGGATAGATTTAGCTTTT CCACATCATGAAAACGAAATTGCACAGTGCGAAGTCTTTCATCAGTGCGAGCAGTGGGGAAATTATTTTC TGCATTCTGGGCATTTGCACGCCAAAGGCAAAGAAGAAAAAATGTCCAAATCATTAAAGAACTACATTAC TATTAAGGACTTTCTGAAGACCTTTTCCCCCGATGTCTTCCGGTTCTTCTGCCTGCGGAGCAGCTACCGC TCAGCCATCGACTACAGTGACAGCGCCATGCTCCAAGCTCAGCAGCTGCTCCTGGGGCTGGGCTCTTTCC TGGAGGACGCACGTGCCTACATGAAGGGGCAGCTGGCCTGCGGCTCCGTCAGGGAAGCGATGCTGTGGGA GAGGCTCTCCAGCACCAAGAGGGCCGTGAAGGCGGCCTTGGCAGATGATTTTGACACACCCAGGGTGGTT GATGCCATCCTGGGCCTTGCACACCACGGGAATGGACAGCTCAGGGCGTCCCTGAAGGAACCTGAAGGGC CGAGAAGTCCTGCTGTGTTTGGTGCCATCATCTCTTACTTTGAACAGTTTTTTGAAACTGTTGGAATTTC TCTGGCAAATCAACAGTACGTTTCAGGAGACGGCAGCGAGGCTACCTTGCATGGTGTGGTGGACGAGCTG GTGCGGTTCCGGCAGAAGGTCCGGCAGTTTGCGCTGGCCATGCCCGAGGCCACGGGGGACGCCCGGCGGC AGCAGCTCCTAGAAAGGCAGCCCCTGCTGGAAGCATGCGACACCCTGCGCCGGGGCCTGACTGCCCACGG CATCAACATCAAGGACAGAAGCAGTACAACATCCACGTGGGAACTGCTGGATCAAAGGACAAAAGACCAA AAATCAGCGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203400 representing NM_024537
Red=Cloning site Green=Tags(s) MLRTTRGPGLGPPLLQAALGLGRAGWHWPAGRAASGGRGRAWLQPTGRETGVQVYNSLTGRKEPLIVAHA EAASWYSCGPTVYDHAHLGHACSYVRFDIIRRILTKVFGCSIVMVMGITDVDDKIIKRANEMNISPASLA SLYEEDFKQDMAALKVLPPTVYLRVTENIPQIISFIEGIIARGNAYSTAKGNVYFDLKSRGDKYGKLVGV VPGPVGEPADSDKRHASDFALWKAAKPQEVFWASPWGPGRPGWHIECSAIASMVFGSQLDIHSGGIDLAF PHHENEIAQCEVFHQCEQWGNYFLHSGHLHAKGKEEKMSKSLKNYITIKDFLKTFSPDVFRFFCLRSSYR SAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKGQLACGSVREAMLWERLSSTKRAVKAALADDFDTPRVV DAILGLAHHGNGQLRASLKEPEGPRSPAVFGAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDEL VRFRQKVRQFALAMPEATGDARRQQLLERQPLLEACDTLRRGLTAHGINIKDRSSTTSTWELLDQRTKDQ KSAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024537 |
| ORF Size | 1692 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024537.4 |
| RefSeq Size | 1865 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 79587 |
| UniProt ID | Q9HA77 |
| Domains | tRNA-synt_1e |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| MW | 62.2 kDa |
| Gene Summary | This gene encodes a putative member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of cysteine to tRNA molecules. A splice-site mutation in this gene has been associated with a novel progressive myoclonic epilepsy disease with similar symptoms to MERRF syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203400L3 | Lenti-ORF clone of CARS2 (Myc-DDK-tagged)-Human cysteinyl-tRNA synthetase 2, mitochondrial (putative) (CARS2), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RC203400L4 | Lenti-ORF clone of CARS2 (mGFP-tagged)-Human cysteinyl-tRNA synthetase 2, mitochondrial (putative) (CARS2), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RG203400 | CARS2 (tGFP-tagged) - Human cysteinyl-tRNA synthetase 2, mitochondrial (putative) (CARS2), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC310263 | CARS2 (untagged)-Human cysteinyl-tRNA synthetase 2, mitochondrial (putative) (CARS2), nuclear gene encoding mitochondrial protein |
CNY 7,220.00 |
