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XLF (NHEJ1) (NM_024782) Human Tagged ORF Clone
CAT＃: RC203393
NHEJ1 (Myc-DDK-tagged)-Human nonhomologous end-joining factor 1 (NHEJ1)
ORF Plasmid: tGFP
"NM_024782" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | XLF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203393 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAACTGGAGCAAGGCCTGTTGATGCAGCCATGGGCGTGGCTACAGCTTGCAGAGAACTCCCTCT TGGCCAAGGTTTTTATCACCAAGCAGGGCTATGCCTTGTTGGTTTCAGATCTTCAACAGGTGTGGCATGA ACAGGTGGACACTAGTGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGGCTCACTGCTCCTCCTGCA GCTTTCCTCTGTCATTTGGATAATCTCCTTCGCCCATTGTTGAAGGACGCTGCTCACCCTAGCGAAGCTA CCTTCTCCTGTGATTGTGTGGCAGATGCACTGATTCTACGGGTGCGAAGTGAGCTCTCTGGCCTCCCCTT CTATTGGAATTTCCACTGCATGCTAGCTAGTCCTTCCCTGGTCTCCCAACATTTGATTCGTCCTCTGATG GGCATGAGTCTGGCATTACAGTGCCAAGTGAGGGAGCTAGCAACGTTACTTCATATGAAAGACCTAGAGA TCCAAGACTACCAGGAGAGTGGGGCTACGCTGATTCGAGATCGATTGAAGACAGAACCATTTGAAGAAAA TTCCTTCTTGGAACAATTTATGATAGAGAAACTGCCAGAGGCATGCAGCATTGGTGATGGAAAGCCCTTT GTCATGAATCTGCAGGATCTGTATATGGCAGTCACCACACAAGAGGTCCAAGTGGGACAGAAGCATCAAG GCGCTGGAGATCCTCATACCTCAAACAGTGCTTCCCTGCAAGGAATCGATAGCCAATGTGTAAACCAGCC AGAACAACTGGTCTCCTCAGCCCCAACCCTCTCAGCACCTGAGAAAGAGTCCACGGGTACTTCAGGCCCT CTGCAGAGACCTCAGCTGTCAAAGGTCAAGAGGAAGAAGCCAAGGGGTCTCTTCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203393 protein sequence
Red=Cloning site Green=Tags(s) MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPA AFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLM GMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPF VMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGP LQRPQLSKVKRKKPRGLFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024782 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024782.3 |
| RefSeq Size | 2119 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 79840 |
| UniProt ID | Q9H9Q4 |
| Protein Pathways | Non-homologous end-joining |
| MW | 33.3 kDa |
| Gene Summary | Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203393L1 | Lenti ORF clone of Human nonhomologous end-joining factor 1 (NHEJ1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203393L2 | Lenti ORF clone of Human nonhomologous end-joining factor 1 (NHEJ1), mGFP tagged |
CNY 5,890.00 |
|
| RC203393L3 | Lenti ORF clone of Human nonhomologous end-joining factor 1 (NHEJ1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203393L4 | Lenti ORF clone of Human nonhomologous end-joining factor 1 (NHEJ1), mGFP tagged |
CNY 5,890.00 |
|
| RG203393 | NHEJ1 (tGFP-tagged) - Human nonhomologous end-joining factor 1 (NHEJ1) |
CNY 4,000.00 |
|
| SC112059 | NHEJ1 (untagged)-Human nonhomologous end-joining factor 1 (NHEJ1) |
CNY 6,270.00 |
