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POLR3F (NM_006466) Human Tagged ORF Clone
CAT＃: RC203100
POLR3F (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F)
ORF Plasmid: tGFP
"NM_006466" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C34; RPC6; RPC39 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203100 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGTGAAGGTGAAGGTGCAGCCGCCTGACGCGGATCCGGTCGAAATAGAAAACAGGATTATAG AATTATGTCACCAGTTCCCTCATGGAATCACAGACCAAGTAATTCAGAATGAAATGCCTCATATAGAAGC CCAGCAGCGGGCAGTAGCCATCAATAGGTTGTTGTCTATGGGTCAGTTGGATCTCTTAAGGAGCAATACG GGCCTTTTATATAGAATAAAGGACTCTCAGAATGCTGGTAAAATGAAGGGATCCGATAACCAAGAAAAAC TAGTATATCAAATCATAGAGGATGCAGGAAATAAAGGAATATGGAGCAGAGATATCCGCTATAAAAGTAA TTTGCCATTAACAGAAATCAACAAAATTCTGAAGAATCTGGAAAGTAAAAAGCTTATCAAAGCTGTTAAG TCTGTAGCAGCCTCAAAAAAGAAGGTGTATATGCTCTATAACCTGCAGCCAGACCGGTCTGTGACTGGTG GAGCCTGGTACAGTGACCAGGATTTTGAATCTGAATTTGTAGAGGTGCTTAACCAACAGTGTTTTAAATT CCTACAGTCCAAGGCAGAAACAGCACGAGAAAGCAAACAGAACCCAATGATACAAAGAAATAGTTCATTT GCCTCATCACATGAAGTGTGGAAATATATCTGCGAATTGGGAATCAGTAAGGTAGAGTTATCCATGGAAG ACATTGAAACCATCCTGAATACACTCATTTATGATGGAAAAGTGGAGATGACGATTATTGCTGCAAAAGA AGGCACAGTTGGCAGTGTAGATGGACACATGAAACTGTACAGGGCAGTCAATCCAATCATCCCTCCCACG GGTTTGGTCCGGGCACCCTGTGGACTCTGCCCGGTTTTTGATGACTGCCACGAAGGTGGTGAGATTTCAC CATCTAACTGTATTTACATGACAGAGTGGCTCGAATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203100 protein sequence
Red=Cloning site Green=Tags(s) MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNT GLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVK SVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSF ASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPT GLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006466 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006466.4 |
| RefSeq Size | 2159 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 10621 |
| UniProt ID | Q9H1D9 |
| Domains | RNA_pol_Rpc34 |
| Protein Families | Transcription Factors |
| Protein Pathways | Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 35.7 kDa |
| Gene Summary | The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Systematic autoantigen analysis identifies a distinct subtype of scleroderma with coincident cancer
,Xu, GJ;Shah, AA;Li, MZ;Xu, Q;Rosen, A;Casciola-Rosen, L;Elledge, SJ;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 27821747
[POLR3F]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203100L3 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203100L4 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), mGFP tagged |
CNY 5,890.00 |
|
| RG203100 | POLR3F (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F) |
CNY 4,000.00 |
|
| SC116048 | POLR3F (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F) |
CNY 2,400.00 |
