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UBC6e (UBE2J1) (NM_016021) Human Tagged ORF Clone
CAT＃: RC202826
UBE2J1 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1)
ORF Plasmid: tGFP
"NM_016021" in other vectors (7)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; UBC6E; Ubc6p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202826 representing NM_016021
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTTAATGAAAGAAGCGGCAGAATTGAAAG ATCCAACAGATCATTACCATGCGCAGCCTTTAGAGGATAACCTTTTTGAATGGCACTTCACGGTTAGAGG GCCCCCAGACTCCGATTTTGATGGAGGAGTTTATCACGGGCGGATAGTACTGCCACCAGAGTATCCCATG AAACCACCAAGCATTATTCTCCTAACGGCTAATGGTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCA TCTCAGGCCATCATCCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCATCATTGG GTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATTACACTCCTGAGGAAAGAAGAGCACTT GCCAAAAAATCACAAGATTTCTGTTGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTGTTGCCTTTAA AATCTGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCTAGGCAAATAAGCTTTAAGGC AGAAGTCAATTCATCTGGAAAGACTATCTCTGAGTCAGACTTAAACCACTCTTTTTCACTAACTGATTTA CAAGATGATATACCTACAACATTCCAGGGTGCTACGGCCAGTACATCGTACGGAGTCCAGAATTCCTCAG CAGCATCCTTTCATCAACCTACCCAACCTGTAGCTAAGAATACCTCCATGAGCCCTCGACAGCGCCGGGC CCAGCAGCAGAGTCAGAGAAGGTTGTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAAC CACACTGATCATGGTGGGTCAGCTGTACTGATTGTCATCCTGACTTTGGCATTGGCAGCTCTTATATTCC GACGAATATATCTGGCAAACGAATACATATTTGACTTTGAGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202826 representing NM_016021
Red=Cloning site Green=Tags(s) METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPM KPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRAL AKKSQDFCCEGCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDL QDDIPTTFQGATASTSYGVQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDN HTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDFEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016021 |
| ORF Size | 954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016021.2, NP_057105.2 |
| RefSeq Size | 4360 bp |
| RefSeq ORF | 957 bp |
| Locus ID | 51465 |
| UniProt ID | Q9Y385 |
| Domains | UBCc |
| Protein Families | Transmembrane |
| Protein Pathways | Parkinson's disease, Ubiquitin mediated proteolysis |
| MW | 35 kDa |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202826L1 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202826L2 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1), mGFP tagged |
CNY 5,890.00 |
|
| RC202826L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202826L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1), mGFP tagged |
CNY 5,890.00 |
|
| RG202826 | UBE2J1 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1) |
CNY 4,000.00 |
|
| SC114499 | UBE2J1 (untagged)-Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1) |
CNY 2,400.00 |
|
| SC320346 | UBE2J1 (untagged)-Human ubiquitin-conjugating enzyme E2, J1, U (UBE2J1) |
CNY 2,400.00 |
