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PSMC6 (NM_002806) Human Tagged ORF Clone
CAT＃: RC202809
PSMC6 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6)
ORF Plasmid: tGFP
"NM_002806" in other vectors (12)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p42; RPT5; SUG2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202809 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCCTAGAGATAAGGCGCTTCAGGACTACCGCAAGAAGTTGCTTGAACACAAGGAGATCGACG GCCGTCTTAAGGAGTTAAGGGAACAATTAAAAGAACTTACCAAGCAGTATGAAAAGTCTGAAAATGATCT GAAGGCCCTACAGAGTGTTGGGCAGATCGTGGGTGAAGTGCTTAAACAGTTAACTGAAGAAAAATTCATT GTTAAAGCTACCAATGGACCAAGATATGTTGTGGGTTGTCGTCGACAGCTTGACAAAAGTAAGCTGAAGC CAGGAACAAGAGTTGCTTTGGATATGACTACACTAACTATCATGAGATATTTGCCGAGAGAGGTGGATCC ACTGGTTTATAACATGTCTCATGAGGACCCTGGGAATGTTTCTTATTCTGAGATTGGAGGGCTATCAGAA CAGATCCGGGAATTAAGAGAGGTGATAGAATTACCTCTTACAAACCCAGAGTTATTTCAGCGTGTAGGAA TAATACCTCCAAAAGGCTGTTTGTTATATGGACCACCAGGTACGGGAAAAACACTCTTGGCACGAGCCGT TGCTAGCCAGCTGGACTGCAATTTCTTAAAGGTTGTATCTAGTTCTATTGTAGACAAGTACATTGGTGAA AGTGCTCGTTTGATCAGAGAAATGTTTAATTATGCTAGAGATCATCAACCATGCATCATTTTTATGGATG AAATAGATGCTATTGGTGGTCGTCGGTTTTCTGAGGGTACTTCAGCTGACAGAGAGATTCAGAGAACGTT AATGGAGTTACTGAATCAAATGGATGGATTTGATACTCTGCATAGAGTTAAAATGATCATGGCTACAAAC AGACCAGATACACTGGATCCTGCTTTGCTGCGTCCAGGAAGATTAGATAGAAAAATACATATTGATTTGC CAAATGAACAAGCAAGATTAGACATACTGAAAATCCATGCAGGTCCCATTACAAAGCATGGTGAAATAGA TTATGAAGCAATTGTGAAGCTTTCGGATGGCTTTAATGGAGCAGATCTGAGAAATGTTTGTACTGAAGCA GGTATGTTCGCAATTCGTGCTGATCATGATTTTGTAGTACAGGAAGACTTCATGAAAGCAGTCAGAAAAG TGGCTGATTCTAAGAAGCTGGAGTCTAAATTGGACTACAAACCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202809 protein sequence
Red=Cloning site Green=Tags(s) MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFI VKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSE QIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGE SARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATN RPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEA GMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002806 |
| ORF Size | 1167 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002806.5 |
| RefSeq Size | 1599 bp |
| RefSeq ORF | 1170 bp |
| Locus ID | 5706 |
| UniProt ID | P62333 |
| Domains | AAA, AAA |
| Protein Pathways | Proteasome |
| MW | 44.2 kDa |
| Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome
,Yi, JJ;Paranjape, SR;Walker, MP;Choudhury, R;Wolter, JM;Fragola, G;Emanuele, MJ;Major, MB;Zylka, MJ;,
J. Biol. Chem.
,PubMed ID 28559284
[PSMC6]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202809L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202809L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6), mGFP tagged |
CNY 5,890.00 |
|
| RC202809L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202809L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6), mGFP tagged |
CNY 5,890.00 |
|
| RC229193 | PSMC6 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6) |
CNY 3,656.00 |
|
| RC229193L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229193L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG202809 | PSMC6 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6) |
CNY 4,370.00 |
|
| RG229193 | PSMC6 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6) |
CNY 4,370.00 |
|
| SC118427 | PSMC6 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6) |
CNY 3,656.00 |
|
| SC320812 | PSMC6 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6) |
CNY 3,656.00 |
|
| SC327828 | PSMC6 (untagged)-Human proteasome (prosome macropain) 26S subunit ATPase 6 (PSMC6) |
CNY 6,560.00 |
