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LIMS1 (NM_004987) Human Tagged ORF Clone
CAT＃: RC202765
LIMS1 (Myc-DDK-tagged)-Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2
ORF Plasmid: tGFP
"NM_004987" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PINCH; PINCH-1; PINCH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202765 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAACGCCCTGGCCAGCGCCACTTGCGAGCGCTGCAAGGGCGGCTTTGCGCCCGCTGAGAAGATCG TGAACAGTAATGGGGAGCTGTACCATGAGCAGTGTTTCGTGTGCGCTCAGTGCTTCCAGCAGTTCCCAGA AGGACTCTTCTATGAGTTTGAAGGAAGAAAGTACTGTGAACATGACTTTCAGATGCTCTTTGCCCCTTGC TGTCATCAGTGTGGTGAATTCACCATTGGCCGAGTTATCAAAGCCATGAATAACAGCTGGCATCCGGAGT GCTTCCGCTGTGACCTCTGCCAGGAAGTTCTGGCAGATATCGGGTTTGTCAAGAATGCTGGGAGACACCT GTGTCGCCCCTGTCATAATCGTGAGAAAGCCAGAGGCCTTGGGAAATACATCTGCCAGAAATGCCATGCT ATCATCGATGAGCAGCCTCTGATATTCAAGAACGACCCCTACCATCCAGACCATTTCAACTGCGCCAACT GCGGGAAGGAGCTGACTGCCGATGCACGGGAGCTGAAAGGGGAGCTATACTGCCTCCCATGCCATGATAA AATGGGGGTCCCCATCTGTGGTGCTTGCCGACGGCCCATCGAAGGGCGCGTGGTGAACGCTATGGGCAAG CAGTGGCATGTGGAGCATTTTGTTTGTGCCAAGTGTGAGAAACCCTTTCTTGGACATCGCCATTATGAGA GGAAAGGCCTGGCATATTGTGAAACTCACTATAACCAGCTATTTGGTGATGTTTGCTTCCACTGCAATCG TGTTATAGAAGGTGGTGTGGTCTCTGCTCTTAATAAGGCCTGGTGCGTGAACTGCTTTGCCTGTTCTACC TGCAACACTAAATTAACACTCAAGAATAAGTTTGTGGAGTTTGACATGAAGCCAGTCTGTAAGAAGTGCT ATGAGAAATTTCCATTGGAGCTGAAGAAAAGACTTAAGAAACTAGCTGAGACCTTAGGAAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202765 protein sequence
Red=Cloning site Green=Tags(s) MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPC CHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHA IIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGK QWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACST CNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004987 |
| ORF Size | 975 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004987.6 |
| RefSeq Size | 4369 bp |
| RefSeq ORF | 978 bp |
| Locus ID | 3987 |
| UniProt ID | P48059 |
| Domains | LIM |
| Protein Families | Druggable Genome |
| MW | 37.2 kDa |
| Gene Summary | The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202765L1 | Lenti ORF clone of Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC202765L2 | Lenti ORF clone of Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC202765L3 | Lenti ORF clone of Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202765L4 | Lenti ORF clone of Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG202765 | LIMS1 (tGFP-tagged) - Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2 |
CNY 4,000.00 |
|
| SC319714 | LIMS1 (untagged)-Human LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2 |
CNY 2,400.00 |
