UBE2D4 (NM_015983) Human Tagged ORF Clone

Name: UBE2D4 (NM_015983) Human Tagged ORF Clone
SKU: RC202473
Price: 1200.00

CAT＃: RC202473

UBE2D4 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_015983" in other vectors (6)

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CNY 1,200.00

CNY 3,705.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100001

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

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PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

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TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA810786

UBE2D4 mouse monoclonal antibody,clone OTI1B1

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HBUCE1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC202473 representing NM_015983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTAAAGCGGATCCAGAAGGAATTAACCGACTTGCAGAGGGATCCTCCTGCCCAGTGTTCTGCAG GACCTGTCGGTGATGACTTGTTCCACTGGCAGGCCACCATCATGGGCCCGAATGACAGTCCTTACCAAGG AGGTGTTTTCTTCCTGACCATCCACTTTCCTACAGATTACCCGTTCAAGCCCCCAAAGGTTGCTTTCACA ACCAAAATTTATCACCCTAATATCAACAGCAATGGCAGCATCTGCCTTGATATCCTGCGGTCTCAGTGGT CTCCAGCGTTGACTGTGTCAAAAGTTCTCTTGTCCATCTGCTCGCTGCTCTGCGACCCCAACCCCGATGA CCCCCTGGTGCCAGAGATAGCACACACCTACAAGGCCGACAGAGAGAAGTACAACAGACTAGCAAGAGAG TGGACACAAAAATATGCTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC202473 representing NM_015983 Red=Cloning site Green=Tags(s) MAVKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFT TKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLARE WTQKYAM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_015983
ORF Size	441 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015983.4
RefSeq Size	1398 bp
RefSeq ORF	444 bp
Locus ID	51619
UniProt ID	Q9Y2X8
Domains	UBCc
Protein Pathways	Ubiquitin mediated proteolysis
MW	16.5 kDa
Gene Summary	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202473L1	Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00 CNY 5,890.00
RC202473L2	Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202473L3	Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202473L4	Lenti ORF clone of Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG202473	UBE2D4 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,800.00 CNY 4,370.00
SC114559	UBE2D4 (untagged)-Human ubiquitin-conjugating enzyme E2D 4 (putative) (UBE2D4)	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 2,950.00