NEIL1 (NM_024608) Human Tagged ORF Clone

CAT#: RC202329

  • TrueORF®

NEIL1 (Myc-DDK-tagged)-Human nei endonuclease VIII-like 1 (E. coli) (NEIL1)

ORF Plasmid: DDK tGFP



  "NM_024608" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


NEIL1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FPG1; hFPG1; NEI1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202329 representing NM_024608
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAGGGCCCCGAGCTGCACCTGGCCAGCCAGTTTGTGAATGAGGCCTGCAGGGCGCTGGTGTTCG
GCGGCTGCGTGGAGAAGTCCTCTGTCAGCCGCAACCCTGAGGTGCCCTTTGAGAGCAGTGCCTACCGCAT
CTCAGCTTCAGCCCGCGGCAAGGAGCTGCGCCTGATACTGAGCCCTCTGCCTGGGGCCCAGCCCCAACAG
GAGCCACTGGCCCTGGTCTTCCGCTTCGGCATGTCCGGCTCTTTTCAGCTGGTGCCCCGCGAGGAGCTGC
CACGCCATGCCCACCTGCGCTTTTACACGGCCCCGCCTGGCCCCCGGCTCGCCCTATGTTTCGTGGACAT
CCGCCGGTTCGGCCGCTGGGACCTTGGGGGAAAGTGGCAGCCGGGCCGCGGGCCCTGTGTCTTGCAGGAG
TACCAGCAGTTCAGGGAGAGTGTGCTACGAAACCTAGCGGATAAGGCCTTTGACCGGCCCATCTGCGAGG
CCCTCCTGGACCAGAGGTTCTTCAATGGCATTGGCAACTATCTGCGGGCAGAGATCCTGTACCGGCTGAA
GATCCCCCCCTTTGAGAAGGCCCGCTCGGTCCTGGAGGCCCTGCAGCAGCACAGGCCGAGCCCGGAGCTG
ACCCTGAGCCAGAAGATAAGGACCAAGCTGCAGAATCCAGACCTGCTGGAGCTATGTCACTCAGTGCCCA
AGGAAGTGGTCCAGTTGGGGGGCAGGGGCTACGGGTCAGAGAGCGGGGAGGAGGACTTTGCTGCCTTTCG
AGCCTGGCTGCGCTGCTATGGCATGCCAGGCATGAGCTCCCTGCAGGACCGGCATGGCCGTACCATCTGG
TTCCAGGGGGATCCTGGACCGTTGGCACCCAAAGGGCGCAAGTCCCGCAAAAAGAAATCCAAGGCCACAC
AGCTGAGTCCTGAGGACAGAGTGGAGGACGCTTTGCCTCCAAGCAAGGCCCCTTCCAGGACACGAAGGGC
AAAGAGAGACCTTCCTAAGAGGACTGCAACCCAGCGGCCTGAGGGGACCAGCCTCCAGCAGGACCCAGAA
GCTCCCACAGTGCCCAAGAAGGGGAGGAGGAAGGGGCGACAGGCAGCCTCTGGCCACTGCAGACCCCGGA
AGGTCAAGGCTGACATCCCATCCTTGGAACCAGAGGGGACCTCAGCCTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202329 representing NM_024608
Red=Cloning site Green=Tags(s)

MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQ
EPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQE
YQQFRESVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPEL
TLSQKIRTKLQNPDLLELCHSVPKEVVQLGGRGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIW
FQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPE
APTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024608
ORF Size 1170 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024608.1, NP_078884.1
RefSeq Size 1896 bp
RefSeq ORF 1173 bp
Locus ID 79661
UniProt ID Q96FI4
Domains Fapy_DNA_glyco
Protein Families Druggable Genome
Protein Pathways Base excision repair
MW 43.7 kDa
Gene Summary This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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