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GNPDA2 (NM_138335) Human Tagged ORF Clone
CAT＃: RC202194
GNPDA2 (Myc-DDK-tagged)-Human glucosamine-6-phosphate deaminase 2 (GNPDA2)
ORF Plasmid: tGFP
"NM_138335" in other vectors (6)
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CNY 1,200.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GNP2; SB52 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTTGTAATTCTTGATAACTATGACTTGGCTAGTGAATGGGCAGCCAAATACATCTGTAATCGCA TCATTCAGTTCAAACCTGGACAGGACAGATATTTTACACTGGGTTTACCAACAGGGAGTACACCTTTAGG ATGCTATAAAAAACTAATAGAATATCATAAGAATGGACACCTTTCTTTTAAATATGTGAAGACCTTTAAT ATGGATGAATATGTAGGACTTCCAAGAAATCATCCTGAAAGCTACCATTCTTATATGTGGAATAATTTTT TTAAGCATATCGATATAGATCCTAATAATGCACATATCCTTGACGGGAATGCTGCAGATTTACAAGCAGA ATGTGATGCTTTTGAAAACAAAATAAAAGAAGCTGGAGGAATAGATCTTTTTGTTGGAGGAATTGGTCCA GATGGTCATATCGCTTTCAATGAGCCTGGATCCAGTTTAGTGTCAAGGACAAGATTAAAGACTCTAGCAA TGGATACCATCTTGGCAAATGCCAAATATTTTGATGGAGATTTATCAAAAGTGTCAACTATGGCTCTAAC TGTTGGTGTGGGGACAGTGATGGATGCTAGAGAAGTAATGATCCTTATAACAGGGGCACACAAGGCATTT GCCCTGTACAAAGCAATAGAAGGAGTCAATCACATGTGGACTGTTTCCGCTTTCCAGCAGCATCCCCGGA CTATTTTTGTATGCGATGAAGATGCTACTTTAGAATTAAGAGTTAAAACTGTGAAATACTTTAAAGGTCT AATGCATGTGCACAATAAACTTGTGGATCCACTATTCAGTATGAAAGATGGAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202194 protein sequence
Red=Cloning site Green=Tags(s) MRLVILDNYDLASEWAAKYICNRIIQFKPGQDRYFTLGLPTGSTPLGCYKKLIEYHKNGHLSFKYVKTFN MDEYVGLPRNHPESYHSYMWNNFFKHIDIDPNNAHILDGNAADLQAECDAFENKIKEAGGIDLFVGGIGP DGHIAFNEPGSSLVSRTRLKTLAMDTILANAKYFDGDLSKVSTMALTVGVGTVMDAREVMILITGAHKAF ALYKAIEGVNHMWTVSAFQQHPRTIFVCDEDATLELRVKTVKYFKGLMHVHNKLVDPLFSMKDGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138335 |
| ORF Size | 420 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138335.3 |
| RefSeq Size | 2313 bp |
| RefSeq ORF | 831 bp |
| Locus ID | 132789 |
| UniProt ID | Q8TDQ7 |
| Domains | Glucosamine_iso |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Metabolic pathways |
| MW | 30.9 kDa |
| Gene Summary | The protein encoded by this gene is an allosteric enzyme that catalyzes the reversible reaction converting D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Variations of this gene have been reported to be associated with influencing body mass index and susceptibility to obesity. A pseudogene of this gene is located on chromosome 9. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202194L1 | Lenti ORF clone of Human glucosamine-6-phosphate deaminase 2 (GNPDA2), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC202194L2 | Lenti ORF clone of Human glucosamine-6-phosphate deaminase 2 (GNPDA2), mGFP tagged |
CNY 5,890.00 |
|
| RC202194L3 | Lenti ORF clone of Human glucosamine-6-phosphate deaminase 2 (GNPDA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202194L4 | Lenti ORF clone of Human glucosamine-6-phosphate deaminase 2 (GNPDA2), mGFP tagged |
CNY 5,890.00 |
|
| RG202194 | GNPDA2 (tGFP-tagged) - Human glucosamine-6-phosphate deaminase 2 (GNPDA2) |
CNY 2,800.00 |
|
| SC128186 | GNPDA2 (untagged)-Human glucosamine-6-phosphate deaminase 2 (GNPDA2) |
CNY 2,400.00 |
