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AlaRS (AARS) (NM_001605) Human Tagged ORF Clone
CAT＃: RC202136
AARS (Myc-DDK-tagged)-Human alanyl-tRNA synthetase (AARS)
ORF Plasmid: tGFP
"NM_001605" in other vectors (6)
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CNY 7,088.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AARS; CMT2N; DEE29; EIEE29 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202136 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCTACTCTAACAGCAAGTGAAATCCGGCAGCGATTTATAGATTTCTTCAAGAGGAACGAGCATA CGTATGTTCACTCGTCTGCCACCATCCCATTGGATGACCCCACTTTGCTCTTTGCCAATGCAGGCATGAA CCAGTTTAAACCCATTTTCCTGAACACAATTGACCCATCTCACCCCATGGCAAAGCTGAGCAGAGCTGCC AATACCCAGAAGTGCATCCGGGCTGGGGGCAAACATAATGACCTGGACGATGTGGGCAAGGATGTCTATC ATCACACCTTCTTCGAGATGCTGGGCTCTTGGTCTTTTGGAGATTACTTTAAGGAATTGGCATGTAAGAT GGCTCTGGAACTCCTCACCCAAGAGTTTGGCATTCCCATTGAAAGACTTTATGTTACTTACTTTGGCGGG GATGAAGCAGCTGGCTTAGAAGCAGATCTGGAATGCAAACAGATCTGGCAAAATTTGGGGCTGGATGACA CCAAAATCCTCCCAGGCAACATGAAGGATAACTTCTGGGAGATGGGTGACACGGGCCCCTGTGGTCCTTG CAGTGAGATCCACTACGACCGGATTGGTGGTCGGGACGCCGCACATCTTGTCAACCAGGACGACCCTAAT GTGCTGGAGATCTGGAACCTTGTGTTCATCCAGTATAACAGGGAAGCTGATGGCATTCTGAAACCTCTTC CCAAGAAAAGCATTGACACAGGGATGGGCCTGGAACGACTGGTATCTGTGCTGCAGAATAAGATGTCCAA CTATGACACTGACCTTTTTGTCCCTTACTTTGAAGCCATTCAGAAGGGCACAGGTGCCCGACCATACACT GGGAAAGTTGGTGCTGAGGATGCCGATGGGATTGACATGGCCTACCGGGTGCTGGCTGACCATGCTCGGA CCATCACTGTGGCACTGGCTGATGGTGGCCGGCCTGACAACACAGGGCGTGGATATGTGTTGAGACGGAT TCTCCGCCGAGCTGTCCGATACGCCCATGAAAAGCTCAATGCCAGCAGGGGCTTCTTTGCTACGTTAGTG GATGTTGTCGTCCAGTCCCTGGGAGATGCATTTCCTGAGCTGAAGAAGGACCCAGACATGGTGAAGGACA TCATTAATGAAGAAGAGGTGCAGTTTCTCAAGACTCTCAGCAGAGGGCGTCGCATCCTGGACAGGAAAAT TCAGAGCCTGGGAGACAGCAAGACCATTCCCGGAGACACTGCTTGGCTCCTCTATGACACCTATGGGTTT CCAGTGGATCTGACTGGACTGATTGCTGAAGAGAAGGGCCTGGTGGTAGACATGGATGGCTTTGAAGAGG AGAGGAAACTGGCCCAGCTGAAATCACAGGGCAAGGGAGCTGGTGGGGAAGACCTCATTATGCTGGACAT TTACGCTATCGAAGAGCTCCGGGCACGGGGTCTGGAGGTCACAGATGATTCCCCAAAGTACAATTACCAT TTGGACTCCAGTGGTAGCTATGTATTTGAGAACACAGTGGCTACGGTGATGGCTCTGCGCAGGGAGAAGA TGTTCGTGGAAGAGGTGTCCACAGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCTGA GCAAGGAGGCCAGATCTATGACGAAGGCTACCTGGTGAAGGTGGATGACAGCAGTGAAGATAAAACAGAG TTTACAGTGAAGAATGCTCAGGTCCGAGGAGGGTATGTGCTACACATTGGAACCATCTACGGTGACCTGA AAGTGGGGGATCAGGTCTGGCTGTTTATTGATGAGCCCCGACGAAGACCCATCATGAGCAACCACACAGC TACGCACATTCTGAACTTCGCCCTGCGCTCAGTGCTTGGGGAAGCTGACCAGAAAGGCTCATTGGTTGCT CCTGACCGCCTCAGATTTGACTTTACTGCCAAGGGAGCCATGTCCACCCAACAGATCAAGAAGGCTGAAG AGATTGCTAATGAGATGATTGAGGCAGCCAAGGCCGTCTATACCCAGGATTGCCCCCTGGCAGCAGCGAA AGCCATCCAGGGCCTACGGGCTGTGTTTGATGAGACCTATCCTGACCCTGTGCGAGTCGTCTCCATTGGG GTCCCGGTGTCCGAGTTGCTGGATGACCCCTCTGGGCCTGCTGGCTCCCTGACTTCTGTTGAGTTCTGTG GGGGAACGCACCTGCGGAACTCGAGTCATGCAGGAGCTTTTGTGATCGTGACGGAAGAAGCCATTGCCAA GGGTATCCGGAGGATTGTGGCTGTCACAGGTGCCGAGGCCCAGAAGGCCCTCAGGAAAGCAGAGAGCTTG AAGAAATGTCTCTCTGTCATGGAAGCCAAAGTGAAGGCTCAGACTGCTCCAAACAAGGATGTGCAGAGGG AGATCGCTGACCTTGGAGAGGCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAGGATGAATTGCGGGA GACTCTCAAATCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCCGATGTCCAGAAACGA GTGTTAGAGAAGACGAAGCAGTTCATCGACAGCAACCCCAACCAGCCTCTTGTCATCCTGGAGATGGAGA GCGGCGCCTCAGCCAAGGCCCTGAATGAAGCCTTGAAGCTCTTCAAGATGCACTCCCCTCAGACTTCTGC CATGCTCTTCACGGTGGACAATGAGGCTGGCAAGATCACGTGCCTGTGTCAAGTCCCCCAGAATGCAGCC AATCGGGGCTTAAAAGCCAGCGAGTGGGTGCAGCAGGTGTCAGGCTTGATGGACGGTAAAGGTGGTGGCA AGGATGTGTCTGCACAGGCCACAGGCAAGAACGTTGGCTGCCTGCAGGAGGCGCTGCAGCTGGCCACTTC CTTCGCCCAGCTGCGCCTCGGGGATGTAAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202136 protein sequence
Red=Cloning site Green=Tags(s) MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAA NTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGG DEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPN VLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYT GKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLV DVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGF PVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYH LDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTE FTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVA PDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIG VPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESL KKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKR VLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAA NRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001605 |
| ORF Size | 2904 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001605.3 |
| RefSeq Size | 3344 bp |
| RefSeq ORF | 2907 bp |
| Locus ID | 16 |
| UniProt ID | P49588 |
| Domains | tRNA-synt_2c, DHHA1 |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| MW | 106.8 kDa |
| Gene Summary | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202136L1 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC202136L2 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged |
CNY 9,030.00 |
|
| RC202136L3 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC202136L4 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged |
CNY 9,030.00 |
|
| RG202136 | AARS (tGFP-tagged) - Human alanyl-tRNA synthetase (AARS) |
CNY 7,890.00 |
|
| SC119146 | AARS (untagged)-Human alanyl-tRNA synthetase (AARS) |
CNY 2,950.00 |
