PAR2 (F2RL1) (NM_005242) Human Tagged ORF Clone
CAT#: RC202121
- TrueORF®
F2RL1 (Myc-DDK-tagged)-Human coagulation factor II (thrombin) receptor-like 1 (F2RL1)
ORF Plasmid: tGFP
"NM_005242" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GPR11; PAR2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202121 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAGCCCCAGCGCGGCGTGGCTGCTGGGGGCCGCCATCCTGCTAGCAGCCTCTCTCTCCTGCAGTG GCACCATCCAAGGAACCAATAGATCCTCTAAAGGAAGAAGCCTTATTGGTAAGGTTGATGGCACATCCCA CGTCACTGGAAAAGGAGTTACAGTTGAAACAGTCTTTTCTGTGGATGAGTTTTCTGCATCTGTCCTCACT GGAAAACTGACCACTGTCTTCCTTCCAATTGTCTACACAATTGTGTTTGTGGTGGGTTTGCCAAGTAACG GCATGGCCCTGTGGGTCTTTCTTTTCCGAACTAAGAAGAAGCACCCTGCTGTGATTTACATGGCCAATCT GGCCTTGGCTGACCTCCTCTCTGTCATCTGGTTCCCCTTGAAGATTGCCTATCACATACATGGCAACAAC TGGATTTATGGGGAAGCTCTTTGTAATGTGCTTATTGGCTTTTTCTATGGCAACATGTACTGTTCCATTC TCTTCATGACCTGCCTCAGTGTGCAGAGGTATTGGGTCATCGTGAACCCCATGGGGCACTCCAGGAAGAA GGCAAACATTGCCATTGGCATCTCCCTGGCAATATGGCTGCTGATTCTGCTGGTCACCATCCCTTTGTAT GTCGTGAAGCAGACCATCTTCATTCCTGCCCTGAACATCACGACCTGTCATGATGTTTTGCCTGAGCAGC TCTTGGTGGGAGACATGTTCAATTACTTCCTCTCTCTGGCCATTGGGGTCTTTCTGTTCCCAGCCTTCCT CACAGCCTCTGCCTATGTGCTGATGATCAGAATGCTGCGATCTTCTGCCATGGATGAAAACTCAGAGAAG AAAAGGAAGAGGGCCATCAAACTCATTGTCTCTGTCCTGGCCATGTACCTGATCTGCTTCACTCCTAGTA ACCTTCTGCTTGTGGTGCATTATTTTCTGATTAAGAGCCAGGGCCAGAGCCATGTCTATGCCCTGTACAT TGTAGCCCTCTGCCTCTCTACCCTTAACAGCTGCATCGACCCCTTTGTCTATTACTTTGTTTCACATGAT TTCAGGGATCATGCAAAGAACGCTCTCCTTTGCCGAAGTGTCCGCACTGTAAAGCAGATGCAAGTATCCC TCACCTCAAAGAAACACTCCAGGAAATCCAGCTCTTACTCTTCAAGTTCAACCACTGTTAAGACCTCCTA T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202121 protein sequence
Red=Cloning site Green=Tags(s) MRSPSAAWLLGAAILLAASLSCSGTIQGTNRSSKGRSLIGKVDGTSHVTGKGVTVETVFSVDEFSASVLT GKLTTVFLPIVYTIVFVVGLPSNGMALWVFLFRTKKKHPAVIYMANLALADLLSVIWFPLKIAYHIHGNN WIYGEALCNVLIGFFYGNMYCSILFMTCLSVQRYWVIVNPMGHSRKKANIAIGISLAIWLLILLVTIPLY VVKQTIFIPALNITTCHDVLPEQLLVGDMFNYFLSLAIGVFLFPAFLTASAYVLMIRMLRSSAMDENSEK KRKRAIKLIVSVLAMYLICFTPSNLLLVVHYFLIKSQGQSHVYALYIVALCLSTLNSCIDPFVYYFVSHD FRDHAKNALLCRSVRTVKQMQVSLTSKKHSRKSSSYSSSSTTVKTSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005242 |
ORF Size | 1191 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005242.6 |
RefSeq Size | 2883 bp |
RefSeq ORF | 1194 bp |
Locus ID | 2150 |
UniProt ID | P55085 |
Domains | 7tm_1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
MW | 44.1 kDa |
Gene Summary | This gene encodes a member of the G-protein coupled receptor 1 family of proteins. The encoded cell surface receptor is activated through proteolytic cleavage of its extracellular amino terminus, resulting in a new amino terminus that acts as a tethered ligand that binds to an extracellular loop domain. Activation of the receptor has been shown to stimulate vascular smooth muscle relaxation, dilate blood vessels, increase blood flow, and lower blood pressure. This protein is also important in the inflammatory response, as well as innate and adaptive immunity. [provided by RefSeq, Jun 2016] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
RAC1B Suppresses TGF-β-Dependent Chemokinesis and Growth Inhibition through an Autoregulatory Feed-Forward Loop Involving PAR2 and ALK5
,Otterbein, H;Mihara, K;Hollenberg, MD;Lehnert, H;Witte, D;Ungefroren, H;,
Cancers (Basel) 2019
,PubMed ID 31434318
[F2RL1]
|
The Role of PAR2 in TGF-β1-Induced ERK Activation and Cell Motility
,Ungefroren, H;Witte, D;Fiedler, C;Gädeken, T;Kaufmann, R;Lehnert, H;Gieseler, F;Rauch, BH;,
Int J Mol Sci
,PubMed ID 29261154
[F2RL1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202121L1 | Lenti ORF clone of Human coagulation factor II (thrombin) receptor-like 1 (F2RL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202121L2 | Lenti ORF clone of Human coagulation factor II (thrombin) receptor-like 1 (F2RL1), mGFP tagged |
CNY 5,890.00 |
|
RC202121L3 | Lenti ORF clone of Human coagulation factor II (thrombin) receptor-like 1 (F2RL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202121L4 | Lenti ORF clone of Human coagulation factor II (thrombin) receptor-like 1 (F2RL1), mGFP tagged |
CNY 5,890.00 |
|
RG202121 | F2RL1 (tGFP-tagged) - Human coagulation factor II (thrombin) receptor-like 1 (F2RL1) |
CNY 4,370.00 |
|
SC116867 | F2RL1 (untagged)-Human coagulation factor II (thrombin) receptor-like 1 (F2RL1) |
CNY 2,950.00 |
|
SC322345 | F2RL1 (untagged)-Human coagulation factor II (thrombin) receptor-like 1 (F2RL1) |
CNY 2,950.00 |