United States
Germany
Japan
United Kingdom
China
IL1 alpha (IL1A) (NM_000575) Human Tagged ORF Clone
CAT＃: RC202084
IL1A (Myc-DDK-tagged)-Human interleukin 1, alpha (IL1A)
ORF Plasmid: tGFP
"NM_000575" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,600.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1F1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202084 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAAGTTCCAGACATGTTTGAAGACCTGAAGAACTGTTACAGTGAAAATGAAGAAGACAGTTCCT CCATTGATCATCTGTCTCTGAATCAGAAATCCTTCTATCATGTAAGCTATGGCCCACTCCATGAAGGCTG CATGGATCAATCTGTGTCTCTGAGTATCTCTGAAACCTCTAAAACATCCAAGCTTACCTTCAAGGAGAGC ATGGTGGTAGTAGCAACCAACGGGAAGGTTCTGAAGAAGAGACGGTTGAGTTTAAGCCAATCCATCACTG ATGATGACCTGGAGGCCATCGCCAATGACTCAGAGGAAGAAATCATCAAGCCTAGGTCAGCACCTTTTAG CTTCCTGAGCAATGTGAAATACAACTTTATGAGGATCATCAAATACGAATTCATCCTGAATGACGCCCTC AATCAAAGTATAATTCGAGCCAATGATCAGTACCTCACGGCTGCTGCATTACATAATCTGGATGAAGCAG TGAAATTTGACATGGGTGCTTATAAGTCATCAAAGGATGATGCTAAAATTACCGTGATTCTAAGAATCTC AAAAACTCAATTGTATGTGACTGCCCAAGATGAAGACCAACCAGTGCTGCTGAAGGAGATGCCTGAGATA CCCAAAACCATCACAGGTAGTGAGACCAACCTCCTCTTCTTCTGGGAAACTCACGGCACTAAGAACTATT TCACATCAGTTGCCCATCCAAACTTGTTTATTGCCACAAAGCAAGACTACTGGGTGTGCTTGGCAGGGGG GCCACCCTCTATCACTGACTTTCAGATACTGGAAAACCAGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202084 protein sequence
Red=Cloning site Green=Tags(s) MAKVPDMFEDLKNCYSENEEDSSSIDHLSLNQKSFYHVSYGPLHEGCMDQSVSLSISETSKTSKLTFKES MVVVATNGKVLKKRRLSLSQSITDDDLEAIANDSEEEIIKPRSAPFSFLSNVKYNFMRIIKYEFILNDAL NQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEI PKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000575 |
| ORF Size | 813 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000575.5 |
| RefSeq Size | 2943 bp |
| RefSeq ORF | 816 bp |
| Locus ID | 3552 |
| UniProt ID | P01583 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus |
| MW | 30.6 kDa |
| Gene Summary | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Interleukin-1 alpha increases anti-tumor efficacy of cetuximab in head and neck squamous cell carcinoma
,Espinosa-Cotton, M;Rodman Iii, SN;Ross, KA;Jensen, IJ;Sangodeyi-Miller, K;McLaren, AJ;Dahl, RA;Gibson-Corley, KN;Koch, AT;Fu, YX;Badovinac, VP;Laux, D;Narasimhan, B;Simons, AL;,
J Immunother Cancer
,PubMed ID 30890189
[IL1 alpha]
|
|
Interleukin-1a activation and localization in lipopolysaccharide-stimulated human monocytes and macrophages
,Carlsen, TG;Kjærsgaard, P;Jørgensen, TL;Foldbjerg, R;Nielsen, ML;Poulsen, TB;Zabieglo, K;Christiansen, G;Birkelund, S;,
J. Immunol. Methods
,PubMed ID 25870118
[IL1 alpha]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202084L1 | Lenti ORF clone of Human interleukin 1, alpha (IL1A), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC202084L2 | Lenti ORF clone of Human interleukin 1, alpha (IL1A), mGFP tagged |
CNY 5,890.00 |
|
| RC202084L3 | Lenti ORF clone of Human interleukin 1, alpha (IL1A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202084L4 | Lenti ORF clone of Human interleukin 1, alpha (IL1A), mGFP tagged |
CNY 6,000.00 |
|
| RG202084 | IL1A (tGFP-tagged) - Human interleukin 1, alpha (IL1A) |
CNY 5,200.00 |
|
| SC324639 | IL1A (untagged)-Human interleukin 1, alpha (IL1A) |
CNY 3,600.00 |
其它IL1 alpha产品
