KRAS (NM_004985) Human Tagged ORF Clone
CAT#: RC201958
KRAS (Myc-DDK-tagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b
ORF Plasmid: tGFP
"NM_004985" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
Cited in 10 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201958 representing NM_004985
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTGCCTTGACGATACAGCTAA TTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGGATTCCTACAGGAAGCAAGTAGTAATTGA TGGAGAAACCTGTCTCTTGGATATTCTCGACACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAG TACATGAGGACTGGGGAGGGCTTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTC ACCATTATAGAGAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAA ATGTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATGGAATTCCT TTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATACATTAGTTCGAGAAATTC GAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGAAAAAGAAGTCAAAGACAAAGTGTGTAAT TATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201958 representing NM_004985
Red=Cloning site Green=Tags(s) MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ YMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIP FIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004985 |
ORF Size | 564 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004985.5 |
RefSeq Size | 5312 bp |
RefSeq ORF | 567 bp |
Locus ID | 3845 |
UniProt ID | P01116 |
Domains | ras, RAS, RHO, RAB |
Protein Families | Druggable Genome |
Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
MW | 21.2 kDa |
Gene Summary | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
Citations (10)
The use of this cDNA Clones has been cited in the following citations: |
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Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer
,Woolston, A;Khan, K;Spain, G;Barber, LJ;Griffiths, B;Gonzalez-Exposito, R;Hornsteiner, L;Punta, M;Patil, Y;Newey, A;Mansukhani, S;Davies, MN;Furness, A;Sclafani, F;Peckitt, C;Jiménez, M;Kouvelakis, K;Ranftl, R;Begum, R;Rana, I;Thomas, J;Bryant, A;Quezada, S;Wotherspoon, A;Khan, N;Fotiadis, N;Marafioti, T;Powles, T;Lise, S;Calvo, F;Guettler, S;von Loga, K;Rao, S;Watkins, D;Starling, N;Chau, I;Sadanandam, A;Cunningham, D;Gerlinger, M;,
Cancer Cell
,PubMed ID 31287991
[KRAS]
|
KRAS G12D or G12V mutation in human brain arteriovenous malformations
,Oka, M;Kushamae, M;Aoki, T;Yamaguchi, T;Kitazato, K;Abekura, Y;Kawamata, T;Mizutani, T;Miyamoto, S;Takagi, Y;,
World Neurosurg
,PubMed ID 30902772
[KRAS]
|
High expression of RAD51 promotes DNA damage repair and survival in KRAS-mutant lung cancer cells
,Hu, J;Zhang, Z;Zhao, L;Li, L;Zuo, W;Han, L;,
BMB Rep
,PubMed ID 30638176
[KRAS]
|
Characterizing the interaction between insulin-like growth factor 2 mRNA-binding protein 1 (IMP1) and KRAS expression
,Mackedenski, S;Wang, C;Li, WM;Lee, CH;,
Biochem. J.
,PubMed ID 30104206
[KRAS]
|
Mutant KRAS promotes liver metastasis of colorectal cancer, in part, by upregulating the MEK-Sp1-DNMT1-miR-137-YB-1-IGF-IR signaling pathway
,Chu, PC;Lin, PC;Wu, HY;Lin, KT;Wu, C;Bekaii-Saab, T;Lin, YJ;Lee, CT;Lee, JC;Chen, CS;,
Oncogene
,PubMed ID 29559746
[KRAS]
|
MicroRNA-622 is a novel mediator of tumorigenicity in melanoma by targeting Kirsten rat sarcoma
,Dietrich, P;Kuphal, S;Spruss, T;Hellerbrand, C;Bosserhoff, AK;,
Pigment Cell Melanoma Res
,PubMed ID 29495114
[KRAS]
|
KRAS induces lung tumorigenesis through microRNAs modulation
,Shi, L;Middleton, J;Jeon, YJ;Magee, P;Veneziano, D;Laganà, A;Leong, HS;Sahoo, S;Fassan, M;Booton, R;Shah, R;Crosbie, PAJ;Garofalo, M;,
Cell Death Dis
,PubMed ID 29440633
[KRAS]
|
Gene Silencing Using Multifunctionalized Gold Nanoparticles for Cancer Therapy
,Fernandes, AR;Baptista, PV;,
Methods Mol. Biol
,PubMed ID 28150211
[KRAS]
|
Metabolic Adaptation to Nutritional Stress in Human Colorectal Cancer
,Miyo, M;Konno, M;Nishida, N;Sueda, T;Noguchi, K;Matsui, H;Colvin, H;Kawamoto, K;Koseki, J;Haraguchi, N;Nishimura, J;Hata, T;Gotoh, N;Matsuda, F;Satoh, T;Mizushima, T;Shimizu, H;Doki, Y;Mori, M;Ishii, H;,
Sci Rep
,PubMed ID 27924922
[KRAS]
|
MiR-124 governs glioma growth and angiogenesis and enhances chemosensitivity by targeting R-Ras and N-Ras
,Shi, Z;Chen, Q;Li, C;Wang, L;Qian, X;Jiang, C;Liu, X;Wang, X;Li, H;Kang, C;Jiang, T;Liu, LZ;You, Y;Liu, N;Jiang, BH;,
Neuro-oncology
,PubMed ID 24861879
[KRAS]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201958L1 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201958L2 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
RC201958L3 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201958L4 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
RG201958 | KRAS (tGFP-tagged) - Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b |
CNY 4,370.00 |
|
SC109374 | KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b |
CNY 3,600.00 |