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Thimet Oligopeptidase (THOP1) (NM_003249) Human Tagged ORF Clone
CAT＃: RC201738
THOP1 (Myc-DDK-tagged)-Human thimet oligopeptidase 1 (THOP1)
ORF Plasmid: tGFP
"NM_003249" in other vectors (5)
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CNY 5,048.00
CNY 5,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EP24.15; MEPD_HUMAN; MP78; TOP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201738 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCCCCCGCAGCCTGTGCAGGAGACATGGCGGACGCAGCATCTCCGTGCTCTGTGGTAAACGACC TGCGGTGGGACCTGAGTGCCCAGCAGATAGAGGAGCGCACCAGGGAGCTCATCGAGCAGACCAAGCGCGT GTATGACCAGGTTGGCACCCAGGAGTTTGAGGACGTGTCCTACGAGAGCACGCTCAAGGCGCTGGCCGAT GTGGAGGTCACCTACACAGTTCAGAGGAATATCCTTGACTTCCCCCAGCATGTTTCCCCCTCCAAGGACA TCCGGACAGCCAGCACAGAGGCCGACAAGAAGCTCTCTGAGTTCGACGTGGAGATGAGCATGAGGGAGGA CGTGTACCAGAGGATCGTGTGGCTCCAGGAGAAAGTTCAGAAGGACTCACTGAGGCCCGAGGCTGCGCGG TACCTGGAGCGGCTAATCAAGCTGGGCCGGAGAAATGGGCTTCACCTCCCCAGAGAGACTCAGGAAAACA TCAAACGCATCAAGAAGAAGCTGAGCCTTCTGTGCATCGACTTCAACAAGAACCTGAACGAGGACACGAC CTTCCTGCCCTTCACGCTCCAGGAGCTAGGAGGGCTCCCCGAGGACTTTCTGAACTCCCTGGAGAAGATG GAGGACGGCAAGTTGAAGGTCACCCTCAAGTACCCCCATTACTTCCCCCTCCTGAAGAAATGCCACGTGC CTGAGACCAGGAGGAAAGTGGAGGAGGCCTTCAACTGCCGGTGCAAGGAGGAGAACTGCGCTATCCTCAA GGAGCTGGTGACGCTGCGGGCCCAGAAGTCCCGCCTGCTGGGGTTCCACACGCACGCCGACTATGTCCTG GAGATGAACATGGCCAAGACCAGCCAGACCGTGGCCACCTTCCTAGATGAGCTGGCGCAGAAGCTGAAGC CCCTGGGGGAGCAGGAGCGTGCGGTGATTCTGGAGCTGAAGCGTGCGGAGTGCGAGCGCCGGGGCCTGCC CTTCGACGGCCGCATCCGTGCCTGGGACATGCGCTACTACATGAACCAGGTGGAGGAGACGCGCTACTGC GTGGACCAGAACCTGCTCAAGGAGTACTTCCCCGTGCAGGTGGTCACGCACGGGCTGCTGGGCATCTACC AGGAGCTCCTGGGGCTGGCCTTCCACCACGAGGAGGGCGCCAGTGCCTGGCATGAGGACGTGCGGCTCTA CACCGCGAGGGACGCGGCCTCGGGGGAGGTGGTCGGCAAGTTCTACCTGGACCTGTACCCGCGGGAAGGA AAGTACGGGCACGCGGCCTGCTTTGGCCTGCAGCCCGGCTGCCTGCGGCAGGATGGGAGCCGCCAGATCG CCATCGCGGCCATGGTGGCCAACTTCACCAAGCCCACAGCCGACGCGCCCTCGCTGCTGCAGCATGACGA GGTGGAGACCTACTTCCATGAGTTTGGCCACGTGATGCACCAGCTCTGCTCCCAGGCGGAGTTCGCCATG TTCAGCGGGACCCACGTGGAGCGGGACTTTGTGGAGGCGCCGTCGCAGATGCTGGAGAACTGGGTGTGGG AGCAGGAGCCGCTGCTGCGGATGTCGCGGCACTACCGCACAGGCAGCGCCGTGCCCCGGGAGCTCCTGGA GAAGCTCATTGAGTCCCGGCAGGCCAACACAGGCCTCTTCAACCTGCGCCAGATCGTCCTCGCCAAGGTG GACCAGGCCCTGCACACGCAGACGGACGCAGACCCCGCCGAGGAGTATGCGCGGCTCTGCCAGGAGATCC TCGGGGTCCCGGCCACGCCAGGAACCAACATGCCTGCAACCTTCGGCCATCTGGCAGGTGGCTACGACGC CCAGTACTACGGGTACCTGTGGAGCGAGGTGTATTCCATGGACATGTTCCACACGCGCTTCAAGCAGGAG GGTGTCCTGAACAGCAAGGTTGGCATGGATTACAGAAGCTGCATCCTGAGACCCGGCGGTTCCGAGGATG CCAGCGCCATGCTGAGGCGCTTCCTGGGCCGTGACCCCAAGCAGGACGCCTTCCTCCTGAGCAAGGGGCT GCAGGTCGGGGGCTGCGAGCCCGAGCCGCAGGTCTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC201738 protein sequence
Red=Cloning site Green=Tags(s) MKPPAACAGDMADAASPCSVVNDLRWDLSAQQIEERTRELIEQTKRVYDQVGTQEFEDVSYESTLKALAD VEVTYTVQRNILDFPQHVSPSKDIRTASTEADKKLSEFDVEMSMREDVYQRIVWLQEKVQKDSLRPEAAR YLERLIKLGRRNGLHLPRETQENIKRIKKKLSLLCIDFNKNLNEDTTFLPFTLQELGGLPEDFLNSLEKM EDGKLKVTLKYPHYFPLLKKCHVPETRRKVEEAFNCRCKEENCAILKELVTLRAQKSRLLGFHTHADYVL EMNMAKTSQTVATFLDELAQKLKPLGEQERAVILELKRAECERRGLPFDGRIRAWDMRYYMNQVEETRYC VDQNLLKEYFPVQVVTHGLLGIYQELLGLAFHHEEGASAWHEDVRLYTARDAASGEVVGKFYLDLYPREG KYGHAACFGLQPGCLRQDGSRQIAIAAMVANFTKPTADAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAM FSGTHVERDFVEAPSQMLENWVWEQEPLLRMSRHYRTGSAVPRELLEKLIESRQANTGLFNLRQIVLAKV DQALHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQE GVLNSKVGMDYRSCILRPGGSEDASAMLRRFLGRDPKQDAFLLSKGLQVGGCEPEPQVC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003249 |
| ORF Size | 2067 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003249.5 |
| RefSeq Size | 2568 bp |
| RefSeq ORF | 2070 bp |
| Locus ID | 7064 |
| UniProt ID | P52888 |
| Domains | Peptidase_M3 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Renin-angiotensin system |
| MW | 78.8 kDa |
| Gene Summary | The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201738L3 | Lenti ORF clone of Human thimet oligopeptidase 1 (THOP1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201738L4 | Lenti ORF clone of Human thimet oligopeptidase 1 (THOP1), mGFP tagged |
CNY 7,448.00 |
|
| RG201738 | THOP1 (tGFP-tagged) - Human thimet oligopeptidase 1 (THOP1) |
CNY 6,370.00 |
|
| SC118078 | THOP1 (untagged)-Human thimet oligopeptidase 1 (THOP1) |
CNY 2,950.00 |
|
| SC324358 | THOP1 (untagged)-Human thimet oligopeptidase 1 (THOP1) |
CNY 2,950.00 |
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