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PGD (NM_002631) Human Tagged ORF Clone
CAT＃: RC201729
PGD (Myc-DDK-tagged)-Human phosphogluconate dehydrogenase (PGD)
ORF Plasmid: tGFP
"NM_002631" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6PGD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201729 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGCTGACATCGCGCTGATCGGATTGGCCGTCATGGGCCAGAACTTAATTCTGAACATGAATG ACCACGGCTTTGTGGTCTGTGCTTTTAATAGGACTGTCTCCAAAGTTGATGATTTCTTGGCCAATGAGGC AAAGGGAACCAAAGTGGTGGGTGCCCAGTCCCTGAAAGAGATGGTCTCCAAGCTGAAGAAGCCCCGGCGG ATCATCCTCCTGGTGAAGGCTGGGCAAGCTGTGGATGATTTCATCGAGAAATTGGTACCATTGTTGGATA CTGGTGACATCATCATTGACGGAGGAAATTCTGAATATAGGGACACCACAAGACGGTGCCGAGACCTCAA GGCCAAGGGAATTTTATTTGTGGGGAGCGGAGTCAGTGGTGGAGAGGAAGGGGCCCGGTATGGCCCATCG CTCATGCCAGGAGGGAACAAAGAAGCGTGGCCCCACATCAAGACCATCTTCCAAGGCATTGCTGCAAAAG TGGGAACTGGAGAACCCTGCTGTGACTGGGTGGGAGATGAGGGAGCAGGCCACTTCGTGAAGATGGTGCA CAACGGGATAGAGTATGGGGACATGCAGCTGATCTGTGAGGCATACCACCTGATGAAAGACGTGCTGGGC ATGGCGCAGGACGAGATGGCCCAGGCCTTTGAGGATTGGAATAAGACAGAGCTAGACTCATTCCTGATTG AAATCACAGCCAATATTCTCAAGTTCCAAGACACCGATGGCAAACACCTGCTGCCAAAGATCAGGGACAG CGCGGGGCAGAAGGGCACAGGGAAGTGGACCGCCATCTCCGCCCTGGAATACGGCGTACCCGTCACCCTC ATTGGAGAAGCTGTCTTTGCTCGGTGCTTATCATCTCTGAAGGATGAGAGAATTCAAGCTAGCAAAAAGC TGAAGGGTCCCCAGAAGTTCCAGTTTGATGGTGATAAGAAATCATTCCTGGAGGACATTCGGAAGGCACT CTACGCTTCCAAGATCATCTCTTACGCTCAAGGCTTTATGCTGCTAAGGCAGGCAGCCACCGAGTTTGGC TGGACTCTCAATTATGGTGGCATCGCCCTGATGTGGAGAGGGGGCTGCATCATTAGAAGTGTATTCCTAG GAAAGATAAAGGATGCATTTGATCGAAACCCGGAACTTCAGAACCTCCTACTGGACGACTTCTTTAAGTC AGCTGTTGAAAACTGCCAGGACTCCTGGCGGCGGGCAGTCAGCACTGGGGTCCAGGCTGGCATTCCCATG CCCTGTTTTACCACTGCCCTCTCCTTCTATGACGGGTACAGACATGAGATGCTTCCAGCCAGCCTCATCC AGGCTCAGCGGGATTACTTCGGGGCTCACACCTATGAACTCTTGGCCAAACCAGGGCAGTTTATCCACAC CAACTGGACAGGCCATGGTGGCACCGTGTCATCCTCGTCATACAATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201729 protein sequence
Red=Cloning site Green=Tags(s) MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKEMVSKLKKPRR IILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPS LMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLG MAQDEMAQAFEDWNKTELDSFLIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTL IGEAVFARCLSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFG WTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPM PCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002631 |
| ORF Size | 1449 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002631.4 |
| RefSeq Size | 1937 bp |
| RefSeq ORF | 1452 bp |
| Locus ID | 5226 |
| UniProt ID | P52209 |
| Domains | 6PGD, NAD_binding_2 |
| Protein Pathways | Glutathione metabolism, Metabolic pathways, Pentose phosphate pathway |
| MW | 53.1 kDa |
| Gene Summary | 6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201729L1 | Lenti ORF clone of Human phosphogluconate dehydrogenase (PGD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201729L2 | Lenti ORF clone of Human phosphogluconate dehydrogenase (PGD), mGFP tagged |
CNY 5,890.00 |
|
| RC201729L3 | Lenti ORF clone of Human phosphogluconate dehydrogenase (PGD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201729L4 | Lenti ORF clone of Human phosphogluconate dehydrogenase (PGD), mGFP tagged |
CNY 5,890.00 |
|
| RG201729 | PGD (tGFP-tagged) - Human phosphogluconate dehydrogenase (PGD) |
CNY 4,370.00 |
|
| SC320261 | PGD (untagged)-Human phosphogluconate dehydrogenase (PGD) |
CNY 2,950.00 |
