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PPAR gamma (PPARG) (NM_138712) Human Tagged ORF Clone
CAT＃: RC201538
PPARG (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1
ORF Plasmid: tGFP
"NM_138712" in other vectors (6)
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CNY 5,488.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201538 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTTGGGATCAGCTCCGTGGATCTCTCCG TAATGGAAGACCACTCCCACTCCTTTGATATCAAGCCCTTCACTACTGTTGACTTCTCCAGCATTTCTAC TCCACATTACGAAGACATTCCATTCACAAGAACAGATCCAGTGGTTGCAGATTACAAGTATGACCTGAAA CTTCAAGAGTACCAAAGTGCAATCAAAGTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGC TCTACAATAAGCCTCATGAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGTGGAGATAA AGCTTCTGGATTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTCTTCCGGAGAACAATCAGA TTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATCCACAAAAAAAGTAGAAATAAATGTCAGT ACTGTCGGTTTCAGAAATGCCTTGCAGTGGGGATGTCTCATAATGCCATCAGGTTTGGGCGGATGCCACA GGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAATCCAGAGTCCGCTGAC CTCCGGGCCCTGGCAAAACATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCAAAGGCGA GGGCGATCTTGACAGGAAAGACAACAGACAAATCACCATTCGTTATCTATGACATGAATTCCTTAATGAT GGGAGAAGATAAAATCAAGTTCAAACACATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGCCATCCGC ATCTTTCAGGGCTGCCAGTTTCGCTCCGTGGAGGCTGTGCAGGAGATCACAGAGTATGCCAAAAGCATTC CTGGTTTTGTAAATCTTGACTTGAACGACCAAGTAACTCTCCTCAAATATGGAGTCCACGAGATCATTTA CACAATGCTGGCCTCCTTGATGAATAAAGATGGGGTTCTCATATCCGAGGGCCAAGGCTTCATGACAAGG GAGTTTCTAAAGAGCCTGCGAAAGCCTTTTGGTGACTTTATGGAGCCCAAGTTTGAGTTTGCTGTGAAGT TCAATGCACTGGAATTAGATGACAGCGACTTGGCAATATTTATTGCTGTCATTATTCTCAGTGGAGACCG CCCAGGTTTGCTGAATGTGAAGCCCATTGAAGACATTCAAGACAACCTGCTACAAGCCCTGGAGCTCCAG CTGAAGCTGAACCACCCTGAGTCCTCACAGCTGTTTGCCAAGCTGCTCCAGAAAATGACAGACCTCAGAC AGATTGTCACGGAACACGTGCAGCTACTGCAGGTGATCAAGAAGACGGAGACAGACATGAGTCTTCACCC GCTCCTGCAGGAGATCTACAAGGACTTGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201538 protein sequence
Red=Cloning site Green=Tags(s) MTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLK LQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIR LKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESAD LRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIR IFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTR EFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQ LKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138712 |
| ORF Size | 1431 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138712.4 |
| RefSeq Size | 1892 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 5468 |
| UniProt ID | P37231 |
| Domains | HOLI, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| Protein Pathways | Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer |
| MW | 54.7 kDa |
| Gene Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A comprehensive strategy for studying protein-metabolite interactions by metabolomics and native mass spectrometry
,Qin, Q;Wang, B;Wang, J;Chang, M;Xia, T;Shi, X;Xu, G;,
Talanta
,PubMed ID 30609583
[PPAR gamma]
|
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A PPARγ-dependent miR-424/503-CD40 axis regulates inflammation mediated angiogenesis
,Lee, A;Papangeli, I;Park, Y;Jeong, HN;Choi, J;Kang, H;Jo, HN;Kim, J;Chun, HJ;,
Sci Rep
,PubMed ID 28566713
[PPAR gamma]
|
|
Sleeping Beauty screen reveals Pparg activation in metastatic prostate cancer
,Ahmad, I;Mui, E;Galbraith, L;Patel, R;Tan, EH;Salji, M;Rust, AG;Repiscak, P;Hedley, A;Markert, E;Loveridge, C;van der Weyden, L;Edwards, J;Sansom, OJ;Adams, DJ;Leung, HY;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 27357679
[PPAR gamma]
|
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Wnt1 Positively Regulates CD36 Expression via TCF4 and PPAR-? in Macrophages
,Wang, S;Sun, Z;Zhang, X;Li, Z;Wu, M;Zhao, W;Wang, H;Chen, T;Yan, H;Zhu, J;,
Cell. Physiol. Biochem.
,PubMed ID 25721714
[PPAR gamma]
|
|
Metformin induces microRNA-34a to down-regulate Sirt1/Pgc-1a/Nrf2 pathway leading to increased susceptibility of wild-type p53 cancer cells to oxidative stress and therapeutic agents
,Do, MT;Kim, HG;Ho Choi, J;Gwang Jeong, H;,
Free Radic. Biol. Med.
,PubMed ID 24970682
[PPAR gamma]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201538L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC201538L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG201538 | PPARG (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 |
CNY 7,088.00 |
|
| SC124177 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 |
CNY 5,488.00 |
|
| SC317792 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 |
CNY 7,220.00 |
|
| SC322218 | PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1 |
CNY 5,488.00 |
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