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RAD54B (NM_012415) Human Tagged ORF Clone
CAT＃: RC201513
- TrueORF®
RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1
ORF Plasmid: tGFP
"NM_012415" in other vectors (6)
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CNY 9,992.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RDH54 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201513 representing NM_012415
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACGATCTGCAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTTATACCTCCAG GAAGAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGATATAAAATTATTTGAGGGTGT TGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTTAGAATATGCAGTTTAAATCTGCCTAGTGAA GAAAGTACTAGAGAAATCAATAACAGAGATAATTGCAGTGGAAAATATTGTTTTGAAGCACCTACACTGG CAACATTAGATCCACCTCATACAGTTCATTCGGCTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGA GAAATCTGATAGCCTAGTTAAATATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAG TGGGAAGGTGATGCTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAAGGCAAAGACA TTGGAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAGGGCCAAACACTGATGATTTG TGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGATGACTTCAGCAGTGGCAGGTGTTTTCAGCTT GGAGGAGGAAGTACTGCTATCTCGCATTCTTCTCAGGTTGCCAGGAAATGTTTCTCTAACCCTTTCAAAA GTGTTTGTAAACCAAGTTCAAAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGCCATGACCC ATATACGCCAAATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATAAGAACTGT TTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAGAAAGAAGGAA TCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGTGGAGCTATTCTTGCTGATGA AATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATCTGGACCCTGCAGTGTCAGGGACCCTATGGA GGCAAGCCAGTAATAAAGAAGACACTAATTGTCACACCTGGAAGCTTGGTGAATAATTGGAAGAAAGAAT TTCAAAAATGGCTAGGAAGTGAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTTGAAGAATT CATCAAGTCTATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGGATCAAATT AAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCCATTAAGACAA CTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGTACTCCAATTCAGAATGATCT GCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGAATATTAGGCTCTTTGTCATCTTATAGGAAA ATATATGAAGAACCCATCATTTTATCGAGAGAACCTTCTGCTTCTGAGGAAGAAAAGGAGTTAGGAGAAA GAAGAGCAGCTGAACTTACTTGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATTATAAATAA ATATCTCCCACCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGCTTTATCGA AAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCCCATCTAATAT GTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAACTCTATAAAGGAAAAGGAATG TAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATACAAAGGCTTGCTAAGTGTGTTTCCTGCTGAC TACAACCCTCTCCTGTTTACTGAAAAGGAGTCAGGAAAACTACAGGTGTTGTCCAAGCTCTTAGCGGTTA TCCACGAACTTCGACCTACTGAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAACATTTTACA AGAAGTATGTAAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTCAAAGGCAG CAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCAAAAGCTGGTG GTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATTGATTGGAATCCAGCCACTGA CATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAATATCCTGTACATATTTACAGACTCCTAACT ACAGGTACAATAGAAGAAAAGATCTATCAAAGGCAGATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCG ACCTCACCAAGACATCTGAACATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACATTACATGA AAGTTCAGATTGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGAT TCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCTAACTCCCTGA AACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGATCATTTAAATCTTACAGATCC TTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTTCAGAATATAACCACTCAAGCTACTGGCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201513 representing NM_012415
Red=Cloning site Green=Tags(s) MRRSAAPSQLQGNSFKKPKFIPPGRSNPGLNEEITKLNPDIKLFEGVAINNTFLPSQNDLRICSLNLPSE ESTREINNRDNCSGKYCFEAPTLATLDPPHTVHSAPKEVAVSKEQEEKSDSLVKYFSVVWCKPSKKKHKK WEGDAVLIVKGKSFILKNLEGKDIGRGIGYKFKELEKIEEGQTLMICGKEIEVMGVISPDDFSSGRCFQL GGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPRHDPYTPNSLVMPRPDKNHQWVFNKNC FPLVDVVIDPYLVYHLRPHQKEGIIFLYECVMGMRMNGRCGAILADEMGLGKTLQCISLIWTLQCQGPYG GKPVIKKTLIVTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKVEEFIKSIFYSVLIISYEMLLRSLDQI KNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQNDLQEFFALIDFVNPGILGSLSSYRK IYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEIINKYLPPKIENVVFCRPGALQIELYR KLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFNSIKEKECSSTCDKNEEKSLYKGLLSVFPAD YNPLLFTEKESGKLQVLSKLLAVIHELRPTEKVVLVSNYTQTLNILQEVCKRHGYAYTRLDGQTPISQRQ QIVDGFNSQHSSFFIFLLSSKAGGVGLNLIGGSHLILYDIDWNPATDIQAMSRVWRDGQKYPVHIYRLLT TGTIEEKIYQRQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGD SLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012415 |
| ORF Size | 2730 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012415.3, NP_036547.1 |
| RefSeq Size | 3074 bp |
| RefSeq ORF | 2733 bp |
| Locus ID | 25788 |
| UniProt ID | Q9Y620 |
| Domains | SNF2_N, DEAD, helicase_C |
| Protein Families | Druggable Genome |
| Protein Pathways | Homologous recombination |
| MW | 103 kDa |
| Gene Summary | The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201513L1 | Lenti-ORF clone of RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 12,392.00 |
|
| RC201513L2 | Lenti-ORF clone of RAD54B (mGFP-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 5,890.00 |
|
| RC201513L3 | Lenti-ORF clone of RAD54B (Myc-DDK-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 5,890.00 |
|
| RC201513L4 | Lenti-ORF clone of RAD54B (mGFP-tagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 5,890.00 |
|
| RG201513 | RAD54B (tGFP-tagged) - Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 11,592.00 |
|
| SC115426 | RAD54B (untagged)-Human RAD54 homolog B (S. cerevisiae) (RAD54B), transcript variant 1 |
CNY 10,008.00 |
