Inhibin beta E chain (INHBE) (NM_031479) Human Tagged ORF Clone

CAT#: RC201426

INHBE (Myc-DDK-tagged)-Human inhibin, beta E (INHBE)

ORF Plasmid: DDK tGFP



  "NM_031479" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201426 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCTCCCTGATGTCCAGCTCTGGCTGGTGCTGCTGTGGGCACTGGTGCGAGCACAGGGGACAGGGT
CTGTGTGTCCCTCCTGTGGGGGCTCCAAACTGGCACCCCAAGCAGAACGAGCTCTGGTGCTGGAGCTAGC
CAAGCAGCAAATCCTGGATGGGTTGCACCTGACCAGTCGTCCCAGAATAACTCATCCTCCACCCCAGGCA
GCGCTGACCAGAGCCCTCCGGAGACTACAGCCAGGGAGTGTGGCTCCAGGGAATGGGGAGGAGGTCATCA
GCTTTGCTACTGTCACAGACTCCACTTCAGCCTACAGCTCCCTGCTCACTTTTCACCTGTCCACTCCTCG
GTCCCACCACCTGTACCATGCCCGCCTGTGGCTGCACGTGCTCCCCACCCTTCCTGGCACTCTTTGCTTG
AGGATCTTCCGATGGGGACCAAGGAGGAGGCGCCAAGGGTCCCGCACTCTCCTGGCTGAGCACCACATCA
CCAACCTGGGCTGGCATACCTTAACTCTGCCCTCTAGTGGCTTGAGGGGTGAGAAGTCTGGTGTCCTGAA
ACTGCAACTAGACTGCAGACCCCTAGAAGGCAACAGCACAGTTACTGGACAACCGAGGCGGCTCTTGGAC
ACAGCAGGACACCAGCAGCCCTTCCTAGAGCTTAAGATCCGAGCCAATGAGCCTGGAGCAGGCCGGGCCA
GGAGGAGGACCCCCACCTGTGAGCCTGCGACCCCCTTATGTTGCAGGCGAGACCATTACGTAGACTTCCA
GGAACTGGGATGGCGGGACTGGATACTGCAGCCCGAGGGGTACCAGCTGAATTACTGCAGTGGGCAGTGC
CCTCCCCACCTGGCTGGCAGCCCAGGCATTGCTGCCTCTTTCCATTCTGCCGTCTTCAGCCTCCTCAAAG
CCAACAATCCTTGGCCTGCCAGTACCTCCTGTTGTGTCCCTACTGCCCGAAGGCCCCTCTCTCTCCTCTA
CCTGGATCATAATGGCAATGTGGTCAAGACGGATGTGCCAGATATGGTGGTGGAGGCCTGTGGCTGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201426 protein sequence
Red=Cloning site Green=Tags(s)

MRLPDVQLWLVLLWALVRAQGTGSVCPSCGGSKLAPQAERALVLELAKQQILDGLHLTSRPRITHPPPQA
ALTRALRRLQPGSVAPGNGEEVISFATVTDSTSAYSSLLTFHLSTPRSHHLYHARLWLHVLPTLPGTLCL
RIFRWGPRRRRQGSRTLLAEHHITNLGWHTLTLPSSGLRGEKSGVLKLQLDCRPLEGNSTVTGQPRRLLD
TAGHQQPFLELKIRANEPGAGRARRRTPTCEPATPLCCRRDHYVDFQELGWRDWILQPEGYQLNYCSGQC
PPHLAGSPGIAASFHSAVFSLLKANNPWPASTSCCVPTARRPLSLLYLDHNGNVVKTDVPDMVVEACGCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031479
ORF Size 1050 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031479.5
RefSeq Size 2453 bp
RefSeq ORF 1053 bp
Locus ID 83729
UniProt ID P58166
Domains TGF-beta
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
MW 38.6 kDa
Gene Summary This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate an inhibin beta subunit. Inhibins have been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. This gene may be upregulated under conditions of endoplasmic reticulum stress, and this protein may inhibit cellular proliferation and growth in pancreas and liver. [provided by RefSeq, Sep 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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