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POLR2D (NM_004805) Human Tagged ORF Clone
CAT＃: RC201116
POLR2D (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D)
ORF Plasmid: tGFP
"NM_004805" in other vectors (4)
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CNY 1,800.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSRBP4; HSRPB4; RBP4; RPB4; RPB16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201116 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGTGGCAGCGATCCGCGGGCTGGCGACGTAGAGGAGGACGCCTCACAGCTCATCTTTCCTA AAGAGTTTGAAACAGCTGAGACACTTCTAAATTCAGAAGTTCATATGCTTCTGGAACATCGAAAGCAGCA GAATGAGAGTGCAGAGGACGAACAGGAGCTCTCAGAAGTCTTCATGAAAACATTAAACTACACAGCCCGT TTCAGTCGTTTCAAAAACAGAGAGACCATTGCCAGTGTTCGTAGCTTGCTACTCCAGAAAAAGCTTCATA AGTTTGAGTTGGCCTGTTTGGCCAACCTTTGCCCAGAGACTGCTGAGGAGTCCAAGGCTCTAATCCCAAG CTTGGAGGGACGGTTTGAAGATGAGGAGCTGCAGCAGATTCTTGATGATATCCAGACAAAGCGCAGCTTT CAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201116 protein sequence
Red=Cloning site Green=Tags(s) MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTAR FSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSF QY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004805 |
| ORF Size | 426 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004805.4 |
| RefSeq Size | 2338 bp |
| RefSeq ORF | 429 bp |
| Locus ID | 5433 |
| UniProt ID | O15514 |
| Domains | RNA_pol_Rpb4, RPOL4c |
| Protein Families | Transcription Factors |
| Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 16.3 kDa |
| Gene Summary | This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201116L3 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201116L4 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D), mGFP tagged |
CNY 5,890.00 |
|
| RG201116 | POLR2D (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D) |
CNY 4,370.00 |
|
| SC117116 | POLR2D (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide D (POLR2D) |
CNY 3,990.00 |
