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Mucolipin 1 (MCOLN1) (NM_020533) Human Tagged ORF Clone
CAT＃: RC201010
MCOLN1 (Myc-DDK-tagged)-Human mucolipin 1 (MCOLN1)
ORF Plasmid: tGFP
"NM_020533" in other vectors (6)
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CNY 3,990.00
| Cited in 7 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MG-2; ML1; ML4; MLIV; MST080; MSTP080; TRP-ML1; TRPM-L1; TRPML1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201010 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCCCGGCGGGTCCGCGCGGCTCAGAGACCGAGCGGCTTCTGACCCCCAACCCCGGGTATGGGA CCCAGGCGGGGCCTTCACCGGCCCCTCCGACACCCCCAGAAGAGGAAGACCTTCGCCGTCGTCTCAAATA CTTTTTCATGAGTCCCTGCGACAAGTTTCGAGCCAAGGGCCGCAAGCCCTGCAAGCTGATGCTGCAAGTG GTCAAGATCCTGGTGGTCACGGTGCAGCTCATCCTGTTTGGGCTCAGTAATCAGCTGGCTGTGACATTCC GGGAAGAGAACACCATCGCCTTCCGACACCTCTTCCTGCTGGGCTACTCGGACGGAGCGGATGACACCTT CGCAGCCTACACGCGGGAGCAGCTGTACCAGGCCATCTTCCATGCTGTGGACCAGTACCTGGCGTTGCCT GACGTGTCACTGGGCCGGTATGCGTATGTCCGTGGTGGGGGTGACCCTTGGACCAATGGCTCAGGGCTTG CTCTCTGCCAGCGGTACTACCACCGAGGCCACGTGGACCCGGCCAACGACACATTTGACATTGATCCGAT GGTGGTTACTGACTGCATCCAGGTGGATCCCCCCGAGCGGCCCCCTCCGCCCCCCAGCGACGATCTCACC CTCTTGGAAAGCAGCTCCAGTTACAAGAACCTCACGCTCAAATTCCACAAGCTGGTCAATGTCACCATCC ACTTCCGGCTGAAGACCATTAACCTCCAGAGCCTCATCAATAATGAGATCCCGGACTGCTATACCTTCAG CGTCCTGATCACGTTTGACAACAAAGCACACAGTGGGCGGATCCCCATCAGCCTGGAGACCCAGGCCCAC ATCCAGGAGTGTAAGCACCCCAGTGTCTTCCAGCACGGAGACAACAGCTTCCGGCTCCTGTTTGACGTGG TGGTCATCCTCACCTGCTCCCTGTCCTTCCTCCTCTGCGCCCGCTCACTCCTTCGAGGCTTCCTGCTGCA GAACGAGTTTGTGGGGTTCATGTGGCGGCAGCGGGGACGGGTCATCAGCCTGTGGGAGCGGCTGGAATTT GTCAATGGCTGGTACATCCTGCTCGTCACCAGCGATGTGCTCACCATCTCGGGCACCATCATGAAGATCG GCATCGAGGCCAAGAACTTGGCGAGCTACGACGTCTGCAGCATCCTCCTGGGCACCTCGACGCTGCTGGT GTGGGTGGGCGTGATCCGCTACCTGACCTTCTTCCACAACTACAATATCCTCATCGCCACACTGCGGGTG GCCCTGCCCAGCGTCATGCGCTTCTGCTGCTGCGTGGCTGTCATCTACCTGGGCTACTGCTTCTGTGGCT GGATCGTGCTGGGGCCCTATCATGTGAAGTTCCGCTCACTCTCCATGGTGTCTGAGTGCCTGTTCTCGCT CATCAATGGGGACGACATGTTTGTGACGTTCGCCGCCATGCAGGCGCAGCAGGGCCGCAGCAGCCTGGTG TGGCTCTTCTCCCAGCTCTACCTTTACTCCTTCATCAGCCTCTTCATCTACATGGTGCTCAGCCTCTTCA TCGCGCTCATCACCGGCGCCTACGACACCATCAAGCATCCCGGCGGCGCAGGCGCAGAGGAGAGCGAGCT GCAGGCCTACATCGCACAGTGCCAGGACAGCCCCACCTCCGGCAAGTTCCGCCGCGGGAGCGGCTCGGCC TGCAGCCTTCTCTGCTGCTGCGGAAGGGACCCCTCGGAGGAGCATTCGCTGCTGGTGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201010 protein sequence
Red=Cloning site Green=Tags(s) MTAPAGPRGSETERLLTPNPGYGTQAGPSPAPPTPPEEEDLRRRLKYFFMSPCDKFRAKGRKPCKLMLQV VKILVVTVQLILFGLSNQLAVTFREENTIAFRHLFLLGYSDGADDTFAAYTREQLYQAIFHAVDQYLALP DVSLGRYAYVRGGGDPWTNGSGLALCQRYYHRGHVDPANDTFDIDPMVVTDCIQVDPPERPPPPPSDDLT LLESSSSYKNLTLKFHKLVNVTIHFRLKTINLQSLINNEIPDCYTFSVLITFDNKAHSGRIPISLETQAH IQECKHPSVFQHGDNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVGFMWRQRGRVISLWERLEF VNGWYILLVTSDVLTISGTIMKIGIEAKNLASYDVCSILLGTSTLLVWVGVIRYLTFFHNYNILIATLRV ALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFAAMQAQQGRSSLV WLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGAGAEESELQAYIAQCQDSPTSGKFRRGSGSA CSLLCCCGRDPSEEHSLLVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020533 |
| ORF Size | 1740 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020533.3 |
| RefSeq Size | 2118 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 57192 |
| UniProt ID | Q9GZU1 |
| Domains | ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 65 kDa |
| Gene Summary | This gene encodes a memberof the transient receptor potential (TRP) cation channel gene family. The transmembrane protein localizes to intracellular vesicular membranes including lysosomes, and functions in the late endocytic pathway and in the regulation of lysosomal exocytosis. The channel is permeable to Ca(2+), Fe(2+), Na(+), K(+), and H(+), and is modulated by changes in Ca(2+) concentration. Mutations in this gene result in mucolipidosis type IV. [provided by RefSeq, Oct 2009] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
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TRPML1 links lysosomal calcium to autophagosome biogenesis through the activation of the CaMKKβ/VPS34 pathway
,Scotto Rosato, A;Montefusco, S;Soldati, C;Di Paola, S;Capuozzo, A;Monfregola, J;Polishchuk, E;Amabile, A;Grimm, C;Lombardo, A;De Matteis, MA;Ballabio, A;Medina, DL;,
Nat Commun
,PubMed ID 31822666
[Mucolipin 1]
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Transient Receptor Potential Mucolipin-1 Channels in Glioblastoma: Role in Patient's Survival
,Morelli, MB;Amantini, C;Tomassoni, D;Nabissi, M;Arcella, A;Santoni, G;,
Cancers (Basel) 2019
,PubMed ID 31013784
[Mucolipin 1]
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Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion
,Sacco, F;Humphrey, SJ;Cox, J;Mischnik, M;Schulte, A;Klabunde, T;Schäfer, M;Mann, M;,
Nat Commun
,PubMed ID 27841257
[Mucolipin 1]
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Autophagosome-lysosome fusion triggers a lysosomal response mediated by TLR9 and controlled by OCRL
,De Leo, MG;Staiano, L;Vicinanza, M;Luciani, A;Carissimo, A;Mutarelli, M;Di Campli, A;Polishchuk, E;Di Tullio, G;Morra, V;Levtchenko, E;Oltrabella, F;Starborg, T;Santoro, M;di Bernardo, D;Devuyst, O;Lowe, M;Medina, DL;Ballabio, A;De Matteis, MA;,
Nat. Cell Biol.
,PubMed ID 27398910
[Mucolipin 1]
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Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB
,Medina, DL;Di Paola, S;Peluso, I;Armani, A;De Stefani, D;Venditti, R;Montefusco, S;Scotto-Rosato, A;Prezioso, C;Forrester, A;Settembre, C;Wang, W;Gao, Q;Xu, H;Sandri, M;Rizzuto, R;De Matteis, MA;Ballabio, A;,
Nat. Cell Biol.
,PubMed ID 25720963
[Mucolipin 1]
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Cellular Zinc Levels Are Modulated By Trpml1-Tmem163 Interaction
,Cuajungco, MP;Basilio, LC;Silva, J;Hart, T;Tringali, J;Chen, CC;Biel, M;Grimm, C;,
Traffic
,PubMed ID 25130899
[Mucolipin 1]
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Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1–/– cells
,Fan Zhang, Ming Xu, Wei-Qing Han, and Pin-Lan Li,
Am J Physiol Cell Physiol, Aug 2011; 301: C421 - C430.
[Mucolipin 1]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201010L1 | Lenti ORF clone of Human mucolipin 1 (MCOLN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201010L2 | Lenti ORF clone of Human mucolipin 1 (MCOLN1), mGFP tagged |
CNY 5,890.00 |
|
| RC201010L3 | Lenti ORF clone of Human mucolipin 1 (MCOLN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201010L4 | Lenti ORF clone of Human mucolipin 1 (MCOLN1), mGFP tagged |
CNY 5,890.00 |
|
| RG201010 | MCOLN1 (tGFP-tagged) - Human mucolipin 1 (MCOLN1) |
CNY 4,370.00 |
|
| SC319381 | MCOLN1 (untagged)-Human mucolipin 1 (MCOLN1) |
CNY 2,950.00 |
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