NME6 (NM_005793) Human Tagged ORF Clone
CAT#: RC200541
NME6 (Myc-DDK-tagged)-Human non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) (NME6)
ORF Plasmid: tGFP
"NM_005793" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | IPIA-ALPHA; NDK 6; NM23-H6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200541 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCAGAATCTGGGGAGTGAGATGGCCTCAATCTTGCGAAGCCCTCAGGCTCTCCAGCTCACTCTAG CCCTGATCAAGCCTGACGCAGTCGCCCATCCACTGATTCTGGAGGCTGTTCATCAGCAGATTCTAAGCAA CAAGTTCCTGATTGTACGAATGAGAGAACTACTGTGGAGAAAGGAAGATTGCCAGAGGTTTTACCGAGAG CATGAAGGGCGTTTTTTCTATCAGAGGCTGGTGGAGTTCATGGCCAGCGGGCCAATCCGAGCCTACATCC TTGCCCACAAGGATGCCATCCAGCTCTGGAGGACGCTCATGGGACCCACCAGAGTGTTCCGAGCACGCCA TGTGGCCCCAGATTCTATCCGTGGGAGTTTCGGCCTCACTGACACCCGCAACACCACCCATGGTTCGGAC TCTGTGGTTTCAGCCAGCAGAGAGATTGCAGCCTTCTTCCCTGACTTCAGTGAACAGCGCTGGTATGAGG AGGAAGAGCCCCAGTTGCGCTGTGGCCCTGTGTGCTATAGCCCAGAGGGAGGTGTCCACTATGTAGCTGG AACAGGAGGCCTAGGACCAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200541 protein sequence
Red=Cloning site Green=Tags(s) MTQNLGSEMASILRSPQALQLTLALIKPDAVAHPLILEAVHQQILSNKFLIVRMRELLWRKEDCQRFYRE HEGRFFYQRLVEFMASGPIRAYILAHKDAIQLWRTLMGPTRVFRARHVAPDSIRGSFGLTDTRNTTHGSD SVVSASREIAAFFPDFSEQRWYEEEEPQLRCGPVCYSPEGGVHYVAGTGGLGPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005793 |
ORF Size | 582 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005793.4 |
RefSeq Size | 1189 bp |
RefSeq ORF | 585 bp |
Locus ID | 10201 |
UniProt ID | O75414 |
Domains | NDK |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
MW | 22 kDa |
Gene Summary | Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6), such as NME6, are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al., 1999 [PubMed 10453732]).[supplied by OMIM, Jul 2010] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix
,null,
Cell & Bioscience
,PubMed ID 34789336
[NME6]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200541L3 | Lenti ORF clone of Human non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) (NME6), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200541L4 | Lenti ORF clone of Human non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) (NME6), mGFP tagged |
CNY 5,890.00 |
|
RG200541 | NME6 (tGFP-tagged) - Human non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) (NME6) |
CNY 4,000.00 |
|
SC320260 | NME6 (untagged)-Human non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) (NME6) |
CNY 2,400.00 |