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PAFAH2 (NM_000437) Human Tagged ORF Clone
CAT＃: RC200355
PAFAH2 (Myc-DDK-tagged)-Human platelet-activating factor acetylhydrolase 2, 40kDa (PAFAH2)
ORF Plasmid: tGFP
"NM_000437" in other vectors (4)
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CNY 3,656.00
CNY 5,320.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSD-PLA2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTCAACCAGTCTGTGGGCTTTCCACCTGTCACAGGACCCCACCTCGTAGGCTGTGGGGATGTGA TGGAGGGTCAGAATCTCCAGGGGAGCTTCTTTCGACTCTTCTACCCCTGCCAAAAGGCAGAGGAGACCAT GGAGCAGCCCCTGTGGATTCCCCGCTATGAGTACTGCACTGGCCTGGCCGAGTACCTGCAGTTTAATAAG CGCTGCGGGGGCTTGCTGTTCAACCTGGCGGTGGGATCTTGTCGCCTGCCTGTTAGCTGGAATGGCCCCT TTAAGACAAAGGACTCTGGATACCCCTTGATCATCTTCTCCCATGGCCTAGGAGCCTTCAGGACTTTGTA TTCAGCCTTCTGCATGGAGCTGGCCTCACGTGGCTTTGTGGTTGCTGTGCCAGAGCACAGGGACCGGTCA GCGGCAACCACCTATTTCTGCAAGCAGGCCCCAGAAGAGAACCAGCCCACCAATGAATCGCTGCAGGAGG AATGGATCCCTTTCCGTCGAGTTGAGGAAGGGGAGAAGGAATTTCATGTTCGGAATCCCCAGGTGCATCA GCGGGTAAGCGAGTGTTTACGGGTGTTGAAGATCCTGCAAGAGGTCACTGCTGGGCAGACTGTCTTCAAC ATCTTGCCTGGTGGCTTGGATCTGATGACTTTGAAGGGCAACATTGACATGAGCCGTGTGGCTGTGATGG GACATTCATTTGGAGGGGCCACAGCTATTCTGGCTTTGGCCAAGGAGACCCAATTTCGGTGTGCGGTGGC TCTGGATGCTTGGATGTTTCCTCTGGAACGTGACTTTTACCCCAAGGCCCGAGGACCTGTGTTCTTTATC AATACTGAGAAATTCCAGACAATGGAGAGTGTCAATTTGATGAAGAAGATATGTGCCCAGCATGAACAGT CTAGGATCATAACCGTTCTTGGTTCTGTTCATCGGAGTCAAACTGACTTTGCTTTTGTGACTGGCAACTT GATTGGTAAATTCTTCTCCACTGAAACCCGTGGGAGCCTGGACCCCTATGAAGGGCAGGAGGTTATGGTA CGGGCCATGTTGGCCTTCCTGCAGAAGCACCTCGACCTGAAAGAAGACTATAATCAATGGAACAACCTTA TTGAAGGCATTGGACCGTCGCTCACCCCAGGGGCCCCCCACCATCTGTCCAGCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200355 protein sequence
Red=Cloning site Green=Tags(s) MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAEETMEQPLWIPRYEYCTGLAEYLQFNK RCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYPLIIFSHGLGAFRTLYSAFCMELASRGFVVAVPEHRDRS AATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFN ILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFI NTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMV RAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000437 |
| ORF Size | 1176 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000437.4 |
| RefSeq Size | 3581 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 5051 |
| UniProt ID | Q99487 |
| Domains | PAF-AH_p_II |
| Protein Families | Druggable Genome |
| Protein Pathways | Ether lipid metabolism, Metabolic pathways |
| MW | 44 kDa |
| Gene Summary | This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Chemical probes for spatially resolved measurement of active enzymes in single cells
,Li, G;Moellering, R;,
Methods in Enzymology
,PubMed ID 31668232
[PAFAH2]
|
|
Elevated truncated oxidized phospholipids as a factor exacerbating ALI in the aging lungs
,Ke, Y;Karki, P;Kim, J;Son, S;Berdyshev, E;Bochkov, VN;Birukova, AA;Birukov, KG;,
FASEB J.
,PubMed ID 30521374
[PAFAH2]
|
|
An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
,Li, G;Montgomery, JE;Eckert, MA;Chang, JW;Tienda, SM;Lengyel, E;Moellering, RE;,
Nat Commun
,PubMed ID 29176560
[PAFAH2]
|
|
Oxidatively Truncated Phospholipids Are Required Agents of Tumor Necrosis Factor a (TNFa)-induced Apoptosis
,Calivarathan Latchoumycandane, Gopal K. Marathe, Renliang Zhang, and Thomas M. McIntyre,
J. Biol. Chem., May 2012; 287: 17693 - 17705.
[PAFAH2]
|
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200355L3 | Lenti ORF clone of Human platelet-activating factor acetylhydrolase 2, 40kDa (PAFAH2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200355L4 | Lenti ORF clone of Human platelet-activating factor acetylhydrolase 2, 40kDa (PAFAH2), mGFP tagged |
CNY 5,890.00 |
|
| RG200355 | PAFAH2 (tGFP-tagged) - Human platelet-activating factor acetylhydrolase 2, 40kDa (PAFAH2) |
CNY 4,370.00 |
|
| SC319395 | PAFAH2 (untagged)-Human platelet-activating factor acetylhydrolase 2, 40kDa (PAFAH2) |
CNY 2,950.00 |
