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ASF1A (NM_014034) Human Tagged ORF Clone
CAT＃: RC200324
ASF1A (Myc-DDK-tagged)-Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A)
ORF Plasmid: tGFP
"NM_014034" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-98; CIA; HSPC146 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200324 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGGTTCAGGTGAACAATGTAGTGGTGCTGGATAACCCTTCTCCTTTCTACAACCCGTTCCAGT TCGAGATCACCTTCGAGTGCATCGAGGACCTGTCTGAAGACTTGGAATGGAAAATTATCTATGTGGGCTC TGCAGAAAGTGAAGAATACGATCAAGTTTTAGACTCTGTTTTAGTGGGTCCTGTTCCCGCAGGAAGGCAT ATGTTTGTATTTCAGGCTGATGCACCTAATCCAGGACTCATTCCAGATGCAGATGCAGTAGGCGTAACTG TTGTGCTAATTACTTGTACCTATCGAGGACAAGAATTTATTAGAGTTGGCTATTATGTAAATAATGAATA TACTGAGACAGAATTAAGGGAAAATCCACCAGTAAAACCAGACTTTTCTAAGCTTCAAAGGAATATTTTG GCATCTAATCCCAGGGTCACAAGATTCCACATTAATTGGGAAGATAACACAGAAAAACTGGAAGATGCAG AGAGCAGTAATCCAAATCTACAGTCACTTCTTTCAACAGATGCATTACCTTCAGCATCAAAGGGATGGTC CACATCAGAAAACTCACTAAATGTCATGTTAGAATCCCACATGGACTGCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200324 protein sequence
Red=Cloning site Green=Tags(s) MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRH MFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNIL ASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014034 |
| ORF Size | 612 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014034.3 |
| RefSeq Size | 2525 bp |
| RefSeq ORF | 615 bp |
| Locus ID | 25842 |
| UniProt ID | Q9Y294 |
| Domains | Anti-silence |
| MW | 23 kDa |
| Gene Summary | This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200324L1 | Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200324L2 | Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), mGFP tagged |
CNY 5,890.00 |
|
| RC200324L3 | Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200324L4 | Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), mGFP tagged |
CNY 5,890.00 |
|
| RG200324 | ASF1A (tGFP-tagged) - Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A) |
CNY 4,000.00 |
|
| SC321540 | ASF1A (untagged)-Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A) |
CNY 2,400.00 |
