STUB1 (NM_005861) Human Tagged ORF Clone
CAT#: RC200310
- TrueORF®
STUB1 (Myc-DDK-tagged)-Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1)
ORF Plasmid: tGFP
"NM_005861" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 4,465.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CHIP; HSPABP2; NY-CO-7; SCA48; SCAR16; SDCCAG7; UBOX1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200310 representing NM_005861.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGGGCAAGGAGGAGAAGGAGGGCGGCGCACGGCTGGGCGCTGGCGGCGGAAGCCCCGAGAAGAGC CCGAGCGCGCAGGAGCTCAAGGAGCAGGGCAATCGTCTGTTCGTGGGCCGAAAGTACCCGGAGGCGGCG GCCTGCTACGGCCGCGCGATCACCCGGAACCCGCTGGTGGCCGTGTATTACACCAACCGGGCCTTGTGC TACCTGAAGATGCAGCAGCACGAGCAGGCCCTGGCCGACTGCCGGCGCGCCCTGGAGCTGGACGGGCAG TCTGTGAAGGCGCACTTCTTCCTGGGGCAGTGCCAGCTGGAGATGGAGAGCTATGATGAGGCCATCGCC AATCTGCAGCGAGCTTACAGCCTGGCCAAGGAGCAGCGGCTGAACTTCGGGGACGACATCCCCAGCGCT CTTCGAATCGCGAAGAAGAAGCGCTGGAACAGCATTGAGGAGCGGCGCATCCACCAGGAGAGCGAGCTG CACTCCTACCTCTCCAGGCTCATTGCCGCGGAGCGTGAGAGGGAGCTGGAAGAGTGCCAGCGAAACCAC GAGGGTGATGAGGACGACAGCCACGTCCGGGCCCAGCAGGCCTGCATTGAGGCCAAGCACGACAAGTAC ATGGCGGACATGGACGAGCTTTTTTCTCAGGTGGATGAGAAGAGGAAGAAGCGAGACATCCCCGACTAC CTGTGTGGCAAGATCAGCTTTGAGCTGATGCGGGAGCCGTGCATCACGCCCAGTGGCATCACCTACGAC CGCAAGGACATCGAGGAGCACCTGCAGCGTGTGGGTCATTTTGACCCCGTGACCCGGAGCCCCCTGACC CAGGAACAGCTCATCCCCAACTTGGCTATGAAGGAGGTTATTGACGCATTCATCTCTGAGAATGGCTGG GTGGAGGACTAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC200310
Blue=ORF Red=Cloning site Green=Tag(s) MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALC YLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSA LRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKY MADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLT QEQLIPNLAMKEVIDAFISENGWVEDY SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC200310 also available, TP300310 |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005861 |
ORF Size | 909 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005861.4 |
RefSeq Size | 1650 bp |
RefSeq ORF | 912 bp |
Locus ID | 10273 |
UniProt ID | Q9UNE7 |
Domains | TPR, U-box |
Protein Families | Druggable Genome |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 34.9 kDa |
Gene Summary | This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2. [provided by RefSeq, Jun 2014] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
Diptoindonesin G antagonizes AR signaling and enhances the efficacy of antiandrogen therapy in prostate cancer.
,null,
The Prostate
,PubMed ID 35322879
[STUB1]
|
Zinc deficiency causes neural tube defects through attenuation of p53 ubiquitylation
,null,
Development (Cambridge, England)
,PubMed ID 30545932
[STUB1]
|
Latent Membrane Protein 1 of Epstein-Barr Virus Promotes RIG-I Degradation Mediated by Proteasome Pathway
,Xu, C;Sun, L;Liu, W;Duan, Z;,
Front Immunol
,PubMed ID 30002655
[STUB1]
|
Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function
,Kristensen, LV;Oppermann, FS;Rauen, MJ;Fog, K;Schmidt, T;Schmidt, J;Harmuth, T;Hartmann-Petersen, R;Thirstrup, K;,
Neurochemistry International
,PubMed ID 29111377
[STUB1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200310L1 | Lenti ORF clone of Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200310L2 | Lenti ORF clone of Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1), mGFP tagged |
CNY 4,800.00 |
|
RC200310L3 | Lenti ORF clone of Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200310L4 | Lenti ORF clone of Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1), mGFP tagged |
CNY 5,890.00 |
|
RG200310 | STUB1 (tGFP-tagged) - Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1) |
CNY 4,000.00 |
|
SC319337 | STUB1 (untagged)-Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase (STUB1) |
CNY 2,400.00 |