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MTHFD1 (NM_005956) Human Tagged ORF Clone
CAT＃: RC200297
MTHFD1 (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1)
ORF Plasmid: tGFP
"NM_005956" in other vectors (4)
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CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CIMAH; MTHFC; MTHFD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200297 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCAGCAGAAATCCTGAACGGGAAGGAGATCTCCGCGCAAATAAGGGCGAGACTGAAAAATCAAG TCACTCAGTTGAAGGAGCAAGTACCTGGTTTCACACCACGCCTGGCAATATTACAGGTTGGCAACAGAGA TGATTCCAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAAGAGATTGGGATCAAAGCCACTCACATT AAGTTACCAAGAACAACCACAGAATCTGAGGTGATGAAGTACATTACATCTTTGAATGAAGACTCTACTG TACATGGGTTCTTAGTGCAGCTACCTTTAGATTCAGAGAATTCCATTAACACTGAAGAAGTGATCAATGC TATTGCACCCGAGAAGGATGTGGATGGATTGACTAGCATCAATGCTGGGAGACTTGCTAGAGGTGACCTC AATGACTGTTTCATTCCTTGTACGCCTAAGGGATGCTTGGAACTCATCAAAGAGACAGGGGTGCCGATTG CCGGAAGGCATGCTGTGGTGGTTGGGCGCAGTAAAATAGTTGGGGCCCCGATGCATGACTTGCTTCTGTG GAACAATGCCACAGTGACCACCTGCCACTCCAAGACTGCCCATCTGGATGAGGAGGTAAATAAAGGTGAC ATCCTGGTGGTTGCAACTGGTCAGCCTGAAATGGTTAAAGGGGAGTGGATCAAACCTGGGGCAATAGTCA TCGACTGTGGAATCAATTATGTCCCAGATGATAAAAAACCAAATGGGAGAAAAGTTGTGGGTGATGTGGC ATACGACGAGGCCAAAGAGAGGGCGAGCTTCATCACTCCTGTTCCTGGCGGCGTAGGGCCCATGACAGTT GCAATGCTCATGCAGAGCACAGTAGAGAGTGCCAAGCGTTTCCTGGAGAAATTTAAGCCAGGAAAGTGGA TGATTCAGTATAACAACCTTAACCTCAAGACACCTGTTCCAAGTGACATTGATATATCACGATCTTGTAA ACCGAAGCCCATTGGTAAGCTGGCTCGAGAAATTGGTCTGCTGTCTGAAGAGGTAGAATTATATGGTGAA ACAAAGGCCAAAGTTCTGCTGTCAGCACTAGAACGCCTGAAGCACCGGCCTGATGGGAAATACGTGGTGG TGACTGGAATAACTCCAACACCCCTGGGAGAAGGGAAAAGCACAACTACAATCGGGCTAGTGCAAGCCCT TGGTGCCCATCTCTACCAGAATGTCTTTGCGTGTGTGCGACAGCCTTCTCAGGGCCCCACCTTTGGAATA AAAGGTGGCGCTGCAGGAGGCGGCTACTCCCAGGTCATTCCTATGGAAGAGTTTAATCTCCACCTCACAG GTGACATCCATGCCATCACTGCAGCTAATAACCTCGTTGCTGCGGCCATTGATGCTCGGATATTTCATGA ACTGACCCAGACAGACAAGGCTCTCTTTAATCGTTTGGTGCCATCAGTAAATGGAGTGAGAAGGTTCTCT GACATCCAAATCCGAAGGTTAAAGAGACTAGGCATTGAAAAGACTGACCCTACCACACTGACAGATGAAG AGATAAACAGATTTGCAAGATTGGACATTGATCCAGAAACCATAACTTGGCAAAGAGTGTTGGATACCAA TGATAGATTCCTGAGGAAGATCACGATTGGACAGGCTCCAACGGAGAAGGGTCACACACGGACGGCCCAG TTTGATATCTCTGTGGCCAGTGAAATTATGGCTGTCCTGGCTCTCACCACTTCTCTAGAAGACATGAGAG AGAGACTGGGCAAAATGGTGGTGGCATCCAGTAAGAAAGGAGAGCCCGTCAGTGCCGAAGATCTGGGGGT GAGTGGTGCACTGACAGTGCTTATGAAGGACGCAATCAAGCCCAATCTCATGCAGACACTGGAGGGCACT CCAGTGTTTGTCCATGCTGGCCCGTTTGCCAACATCGCACATGGCAATTCCTCCATCATTGCAGACCAGA TCGCACTCAAGCTTGTTGGCCCAGAAGGGTTTGTAGTGACGGAAGCAGGATTTGGAGCAGACATTGGAAT GGAAAAGTTTTTTAACATCAAATGCCGGTATTCCGGCCTCTGCCCCCACGTGGTGGTGCTTGTTGCCACT GTCAGGGCTCTCAAGATGCACGGGGGCGGCCCCACGGTCACTGCTGGACTGCCTCTTCCCAAGGCTTACA TACAGGAGAACCTGGAGCTGGTTGAAAAAGGCTTCAGTAACTTGAAGAAACAAATTGAAAATGCCAGAAT GTTTGGAATTCCAGTAGTAGTGGCCGTGAATGCATTCAAGACGGATACAGAGTCTGAGCTGGACCTCATC AGCCGCCTTTCCAGAGAACATGGGGCTTTTGATGCCGTGAAGTGCACTCACTGGGCAGAAGGGGGCAAGG GTGCCTTAGCCCTGGCTCAGGCCGTCCAGAGAGCAGCACAAGCACCCAGCAGCTTCCAGCTCCTTTATGA CCTCAAGCTCCCAGTTGAGGATAAAATCAGGATCATTGCACAGAAGATCTATGGAGCAGATGACATTGAA TTACTTCCCGAAGCTCAACACAAAGCTGAAGTCTACACGAAGCAGGGCTTTGGGAATCTCCCCATCTGCA TGGCTAAAACACACTTGTCTTTGTCTCACAACCCAGAGCAAAAAGGTGTCCCTACAGGCTTCATTCTGCC CATTCGCGACATCCGCGCCAGCGTTGGGGCTGGTTTTCTGTACCCCTTAGTAGGAACGATGAGCACAATG CCTGGACTCCCCACCCGGCCCTGTTTTTATGATATTGATTTGGACCCTGAAACAGAACAGGTGAATGGAT TATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200297 protein sequence
Red=Cloning site Green=Tags(s) MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHI KLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDL NDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGD ILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTV AMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGE TKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGI KGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFS DIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQ FDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGT PVFVHAGPFANIAHGNSSIIADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVAT VRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLI SRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIE LLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTM PGLPTRPCFYDIDLDPETEQVNGLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005956 |
| ORF Size | 2805 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005956.2, NP_005947.2 |
| RefSeq Size | 3466 bp |
| RefSeq ORF | 2808 bp |
| Locus ID | 4522 |
| UniProt ID | P11586 |
| Domains | FTHFS, THF_DHG_CYH |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate |
| MW | 101.5 kDa |
| Gene Summary | This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200297L3 | Lenti ORF clone of Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), Myc-DDK-tagged |
CNY 9,880.00 |
|
| RC200297L4 | Lenti ORF clone of Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1), mGFP tagged |
CNY 9,248.00 |
|
| RG200297 | MTHFD1 (tGFP-tagged) - Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1) |
CNY 8,740.00 |
|
| SC116397 | MTHFD1 (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (MTHFD1) |
CNY 2,950.00 |
