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TSG101 (NM_006292) Human Tagged ORF Clone
CAT＃: RC200275
- TrueORF®
TSG101 (Myc-DDK-tagged)-Human tumor susceptibility gene 101 (TSG101)
ORF Plasmid: tGFP
"NM_006292" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TSG10; VPS23 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200275 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTAACTGTACGTG AAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGATTCATATGTTTTTAACGATGG CAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCTGTGCCTTATAGAGGTAATACATACAATATT CCAATATGCCTATGGCTACTGGACACATACCCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTT CAATGACTATTAAAACAGGAAAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATG GAAACACCCACAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAATACTTCCTACA TGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCCAATCCCAGTGGTTACCCAGG CTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACAAGTTCTCAGTACCCTTCTCAGCCTCCTGTG ACCACTGTTGGTCCCAGTAGGGATGGCACAATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGG TCAGTGACAAACTGAGATGGCGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAA ACGAACAGAAGAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCTGCTCTGGAAA AAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCCACAGCTCCCTTATACAAACA GATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGACACTATCCTTTACTTGGGAGAAGCCTTGAGA AGGGGCGTGATAGACCTGGATGTCTTCCTGAAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGA GGGCACTAATGCAAAAAGCAAGAAAGACTGCCGGTCTCAGTGACCTCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200275 protein sequence
Red=Cloning site Green=Tags(s) MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNI PICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPP VFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPV TTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQE VAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTILYLGEALR RGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006292 |
| ORF Size | 1170 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006292.4 |
| RefSeq Size | 1562 bp |
| RefSeq ORF | 1173 bp |
| Locus ID | 7251 |
| UniProt ID | Q99816 |
| Domains | UBCc |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Endocytosis |
| MW | 43.9 kDa |
| Gene Summary | The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200275L1 | Lenti ORF clone of Human tumor susceptibility gene 101 (TSG101), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200275L2 | Lenti ORF clone of Human tumor susceptibility gene 101 (TSG101), mGFP tagged |
CNY 5,890.00 |
|
| RC200275L3 | Lenti ORF clone of Human tumor susceptibility gene 101 (TSG101), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200275L4 | Lenti ORF clone of Human tumor susceptibility gene 101 (TSG101), mGFP tagged |
CNY 5,890.00 |
|
| RG200275 | TSG101 (tGFP-tagged) - Human tumor susceptibility gene 101 (TSG101) |
CNY 4,370.00 |
|
| SC116200 | TSG101 (untagged)-Human tumor susceptibility gene 101 (TSG101) |
CNY 2,950.00 |
