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Nucleostemin (GNL3) (NM_014366) Human Tagged ORF Clone
CAT＃: RC200066
GNL3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1
ORF Plasmid: tGFP
"NM_014366" in other vectors (4)
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CNY 4,088.00
CNY 5,320.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C77032; E2IG3; NNP47; NS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200066 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAGGCCTAAGTTAAAGAAAGCAAGTAAACGCATGACCTGCCATAAGCGGTATAAAATCCAAAAAA AGGTTCGAGAACATCATCGAAAATTAAGAAAGGAGGCTAAAAAGCAGGGTCACAAGAAGCCTAGGAAAGA CCCAGGAGTTCCAAACAGTGCTCCCTTTAAGGAGGCTCTTCTTAGGGAAGCTGAGCTAAGGAAACAGAGG CTTGAAGAACTAAAACAGCAGCAGAAACTTGACAGGCAGAAGGAACTAGAAAAGAAAAGAAAACTTGAAA CTAATCCTGATATTAAGCCATCAAATGTGGAACCTATGGAAAAGGAGTTTGGGCTTTGCAAAACTGAGAA CAAAGCCAAGTCGGGCAAACAGAATTCAAAGAAGCTGTACTGCCAAGAACTTAAAAAGGTGATTGAAGCC TCCGATGTTGTCCTAGAGGTGTTGGATGCCAGAGATCCTCTTGGTTGCAGATGTCCTCAGGTAGAAGAGG CCATTGTCCAGAGTGGACAGAAAAAGCTGGTACTTATATTAAATAAATCAGATCTGGTACCAAAGGAGAA TTTGGAGAGCTGGCTAAATTATTTGAAGAAAGAATTGCCAACAGTGGTGTTCAGAGCCTCAACAAAACCA AAGGATAAAGGGAAGATAACCAAGCGTGTGAAGGCAAAGAAGAATGCTGCTCCATTCAGAAGTGAAGTCT GCTTTGGGAAAGAGGGCCTTTGGAAACTTCTTGGAGGTTTTCAGGAAACTTGCAGCAAAGCCATTCGGGT TGGAGTAATTGGTTTCCCAAATGTGGGGAAAAGCAGCATTATCAATAGCTTAAAACAAGAACAGATGTGT AATGTTGGTGTATCCATGGGGCTTACAAGGAGCATGCAAGTTGTCCCCTTGGACAAACAGATCACAATCA TAGATAGTCCGAGCTTCATCGTATCTCCACTTAATTCCTCCTCTGCGCTTGCTCTGCGAAGTCCAGCAAG TATTGAAGTAGTAAAACCGATGGAGGCTGCCAGTGCCATCCTTTCCCAGGCTGATGCTCGACAGGTAGTA CTGAAATATACTGTCCCAGGCTACAGGAATTCTCTGGAATTTTTTACTATGCTTGCTCAGAGAAGAGGTA TGCACCAAAAAGGTGGAATCCCAAATGTTGAAGGTGCTGCCAAACTGCTGTGGTCTGAGTGGACAGGTGC CTCATTAGCTTACTATTGCCATCCCCCTACATCTTGGACTCCTCCTCCATATTTTAATGAGAGTATTGTG GTAGACATGAAAAGCGGCTTCAATCTGGAAGAACTGGAAAAGAACAATGCACAGAGCATAAGAGCCATCA AGGGCCCTCATTTGGCCAATAGCATCCTTTTCCAGTCTTCCGGTCTGACAAATGGAATAATAGAAGAAAA GGACATACATGAAGAATTGCCAAAACGGAAAGAAAGGAAGCAGGAGGAGAGGGAGGATGACAAAGACAGT GACCAGGAAACTGTTGATGAAGAAGTTGATGAAAACAGCTCAGGCATGTTTGCTGCAGAAGAGACAGGGG AGGCACTGTCTGAGGAGACTACAGCAGGTGAACAGTCTACAAGGTCTTTTATCTTGGATAAAATCATTGA AGAGGATGATGCTTATGACTTCAGTACAGATTATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200066 protein sequence
Red=Cloning site Green=Tags(s) MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKQGHKKPRKDPGVPNSAPFKEALLREAELRKQR LEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEA SDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKP KDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMC NVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVV LKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIV VDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDS DQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014366 |
| ORF Size | 1647 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014366.3 |
| RefSeq Size | 2048 bp |
| RefSeq ORF | 1650 bp |
| Locus ID | 26354 |
| UniProt ID | Q9BVP2 |
| Domains | MMR_HSR1 |
| Protein Families | ES Cell Differentiation/IPS, Stem cell - Pluripotency |
| MW | 62 kDa |
| Gene Summary | The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200066L3 | Lenti ORF clone of Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200066L4 | Lenti ORF clone of Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200066 | GNL3 (tGFP-tagged) - Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1 |
CNY 4,370.00 |
|
| SC321341 | GNL3 (untagged)-Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1 |
CNY 4,096.00 |
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