Tsc2 (NM_001286718) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nafld; Tcs2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231869 representing NM_001286718 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCAACAAGCAAAGATTCCGGCTTGAAGGAGAAGTTCAAGATACTGTTGGGATTGGGAACAT CGAGGCCAAATCCCAGGTGTGCAGAAGGCAAACAGACTGAGTTTATCATCACATCGGAAATCTTGAGAGA ACTGAGTGGTGAATGCGGCCTCAACAATCGCATCCGAATGATAGGGCAGATCTGTGATGTGGCAAAAACT AAGAAGCTTGAGGAGCATGCAGTGGAGGCACTTTGGAAGGCTGTCTCAGACTTGCTACAGCCAGAGCGGC CACCAGAGGCCCGGCATGCAGTTCTCACCTTATTGAAGGCCATTGTACAGGGACAGGGTGATCGTTTGGG AGTTCTCAGAGCCCTCTTCTTCAAAGTGATCAAGGACTACCCCTCCAATGAAGACCTCCATGAAAGGCTA GAAGTTTTTAAGGCCCTCACAGACAATGGGAGGCACATTACCTATTTGGAAGAAGAACTGGCAGAGTTTG TCCTGCAGTGGATGGATGTTGGCTTGTCCTCAGAATTCCTTCTGGTACTTGTCAACCTGGTCAAGTTCAA CAGCTGTTACCTTGACGAATACATTGCATCAATGGTTCACATGATTTGTCTGCTATGCATCCGGACAGTG TCCTCTGTGGACATTGAGGTGTCCTTGCAAGTGCTGGATGCTGTGGTCTGCTACAACTGCTTACCAGCCG AGAGCCTGCCTCTGTTCATTATCACCCTGTGCCGCACCATCAATGTCAAGGAGCTGTGTGAGCCTTGCTG GAAGTTGATGCGTAACCTTCTGGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCGCATCATG GAGGACAGATCCTACATGGAAGATGCCCCACTGCTGAGAGGAGCTGTGTTCTTTGTAGGGATGGCACTCT GGGGAGCTCACCGGCTCTACTCTCTCAAGAACTCCCCCACATCTGTGCTGCCGTCTTTTTATGAGGCTAT GACCTGTCCCAATGAGGTGGTGTCATATGAGATTGTTCTGTCCATAACAAGACTCATCAAGAAGTATAGG AAGGAGCTCCAGGCTGTGACATGGGATATTCTGCTGGACATCATTGAACGACTACTTCAGCAACTCCAGA ACCTGGACAGCCCGGAACTCAAGACCATCGTCCATGACCTGCTGACCACTGTGGAGGAGCTATGTGACCA GAACGAGTTCCATGGCTCGCAGGAAAGATACTATGAACTGGTGGAGAGCTATGCAGACCAGAGACCTGAA TCCTCTCTCTTAAACTTGATATCCTACAGAGCCCAGTCCATCCACCCTGCCAAGGATGGCTGGATCCAGA ACTTGCAGTTGTTGATGGAGAGGTTCTTCAGGAATGAGTGCCGCAGCGCCGTGCGCATCAAGGTGTTGGA TGTTCTGTCCTTCGTGCTGCTGATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCGGTGGTCATC TCGCAGCTCTCCCACATTCCCGAGGATAAGGACCATCAGGTCCGAAAGCTGGCTACTCAGCTGCTGGTGG ACCTGGCAGAGGGGTGCCACACCCACCACTTCAACAGTCTGCTGGACATCATTGAAAAGGTGATGGCGCG CTCACTCTCTCCACCCCCGGAGCTGGAAGAAAGGGACCTGGCCATGCACTCGGCCTCCCTGGAGGACGTG AAGACCGCGGTCCTGGGGCTCCTGGTCATCCTTCAGACCAAGCTCTACACCTTGCCTGCCAGCCACGCCA CTCGAGTGTATGAGAGCCTCATTAGTCACATCCAGCTCCATTACAAGCACGGCTACTCCCTGCCCATTGC TAGCAGCATCCGACTACAGGCCTTTGACTTCCTGCTGCTACTGCGGGCTGACTCGCTGCATCGACTGGGC CTGCCCAACAAGGATGGGGTCGTGAGATTCAGCCCTTACTGCCTCTGTGACTGCATGGAACTGGATAGAG CCTCAGAGAAGAAAGCCAGTGGGCCCCTTTCACCTCCAACTGGGCCCCCTAGCCCTGTGCCTATGGGCCC TGCTGTGCGGCTCGGCTACCTACCCTACTCCCTGCTCTTCCGTGTCCTGTTGCAGTGTTTGAAGCAGGAG AGCGACTGGAAGGTGCTGAAGCTGGTGCTCAGCAGGCTGCCGGAGTCACTGCGCTACAAAGTCCTCATCT TCACCTCCCCCTGCAGTGTCGACCAGCTGTCTTCTGCCCTCTGCTCCATGCTTTCAGCTCCAAAGACCCT TGAGCGGCTCCGAGGTACCCCAGAAGGCTTCTCGAGAACTGACCTGCACTTGGCTGTGGTTCCCGTGCTA ACAGCATTAATATCTTATCACAACTATTTGGACAAGACCAGACAGCGTGAGATGGTGTACTGCCTGGAAC AAGGCCTTATCTACCGCTGCGCCAGCCAGTGCGTGGTAGCCTTGGCCATCTGCAGTGTGGAGATGCCCGA CATCATCATCAAGGCGCTTCCTGTCCTGGTGGTGAAGCTCACACACATTTCTGCCACAGCCAGCATGGCC ATCCCGCTCCTGGAGTTCCTGTCTACTTTGGCCAGGCTGCCCCACCTCTACAGGAACTTTGCTGCAGAGC AGTATGCCAGCGTGTTTGCCATCTCCTTGCCATACACCAACCCCTCCAAGTTCAACCAGTACATTGTGTG TCTGGCTCACCACGTCATAGCCATGTGGTTCATCAGGTGCCGACTGCCCTTTCGGAAGGATTTCGTCCCT TATATCACTAAGGGTTTGCGTTCCAATGTCCTCCTGTCTTTTGATGATACCCCTGAGAAGGACAGTTTCA GAGCACGGAGCACCAGTCTTAATGAGAGACCCAAAAGAATACAGACATCCCTTACGAGCGCCAGCCTGGG GTCTGCGGATGAGAACTCTATGGCCCAGGCTGATGACAACTTGAAGAATCTCCACTTGGAGCTCACAGAA ACATGTCTGGACATGATGGCCCGATATGTGTTCTCCAATTTCACTGCAGTCCCCAAGAGGTCCCCTGTGG GAGAGTTCCTCCTGGCAGGGGGTAGGACCAAAACCTGGCTGGTTGGAAACAAGCTTGTCACTGTGACAAC AAGTGTGGGAACTGGTACACGGTCGCTGCTGGGCCTGGACTCTGGGGACCTGCAGGGTGGCTCGGATTCA AGCTCTGATCCTAGCACACATGTGAGGCAGACAAAGGAAGCACCGGCCAAACTGGAGTCCCAGGCTGGGC AGCAGGTGTCCCGTGGGGCCCGGGACCGGGTCCGCTCCATGTCAGGGGGCCATGGCCTTCGAGTTGGTGT CCTGGATACTTCAGCTCCCTATTCCCCAGGTGGCTCTGCTTCTCTGGGACCACAGACGGCGGTGGCAGCG AAGCCCGAGAAGCCCCCTGCAGGAGCCCAGCTTCCAACAGCAGAGAAGACGAATCTGGCAGCCTATGTGC CTTTGTTAACCCAGGGCTGGGCAGAAATCTTAGTCCGCAGACCCACAGGAAACACCAGCTGGCTGATGAG CTTGGAGAACCCGCTCAGCCCCTTCTCCTCAGACATCAACAACATGCCCCTGCAAGAGCTGTCCAATGCC CTTATGGCTGCTGAACGTTTCAAGGAGCACCGGGACACGGCCCTGTACAAGTCATTGTCAGTGCCAGCGG CTGGCACCGCCAAGCCTCCTACTCTCCCACGCTCTAATACAGACTCAGCCATGGTCCTTGAGGAGGGAAG TCCAGGAGAGACTCAGGTGCCAGTGGAGCCCCCCGAATTGGAGGATTTCGAGGCGGCGTTAGGTACAGAC AGGCACTGCCAGCGGCCTGACACCTACAGCCGGTCATCCTCAGCATCTAGCCAGGAAGAAAAGTCCCATT TGGAGGAGCTTGCTGCAGGGGGTATCCCCATTGAGCGGGCCATCTCCTCTGAGGGGGCTCGGCCTGCCGT GGACCTCTCCTTCCAACCCTCACAGCCTTTGAGCAAGTCTAGCTCTTCTCCGGAGTTGCAGACCTTACAG GACATCCTTGGAGACCTAGGGGACAAGATTGATATTGGACGGCTGAGTCCTGAGGCTAAGGTCCGGTCCC AGTCAGGGATCCTGGATGGGGAAGCAGCTACCTGGTCAGCTACGGGTGAAGAGAGCCGTATCACAGTCCC ACCTGAAGGTCCTCTGCCTTCCAGTTCTCCCCGCTCCCCCAGTGGCCTCCGGCCCCGAGGCTATACCATC TCTGATTCCGCTCCATCACGAAGGGGAAAGAGGGTAGAAAGGGATAACTTCAAGAGCAGAGCTGCGGCCT CCAGTGCTGAGAAGGTGCCAGGCATCAACCCTAGCTTTGTGTTCCTACAGCTCTACCATTCGCCCTTTTT TGGTGATGAGTCCAATAAGCCCATCCTATTGCCCAATGAGTCCTTTGAACGGTCAGTACAGCTGCTTGAC CAGATTCCATCCTATGACACTCACAAGATTGCTGTCCTGTATGTGGGAGAAGGCCAGAGCAGCAGTGAGC TGGCCATCCTGTCAAATGAGCATGGCTCTTACAGGTACACGGAGTTTCTGACAGGCCTGGGTCGGCTTAT TGAGCTCAAGGACTGCCAACCAGACAAGGTGTACTTAGGTGGACTGGATGTATGTGGCGAGGATGGACAG TTCACCTACTGCTGGCATGATGACATCATGCAAGCTGTTTTCCACATTGCCACCCTGATGCCCACCAAGG ATGTGGACAAGCACCGCTGTGACAAGAAACGGCACCTGGGCAATGACTTTGTTTCTATCATCTACAATGA TTCTGGTGAGGACTTCAAACTGGGCACCATTAAGGGCCAGTTCAACTTTGTCCATGTGATCATCACACCG CTGGACTACAAGTGCAACCTATTGACCCTGCAGTGCAGGAAAGACATGGAAGGCCTCGTGGACACCAGTG TGGCCAAGATTGTGTCTGACCGCAACCTGTCCTTTGTGGCCCGACAGATGGCCCTGCATGCAAATATGGC CTCACAGGTACACCACAGCCGATCCAACCCCACTGACATCTATCCCTCCAAGTGGATCGCAAGACTCCGC CACATTAAGCGTCTCCGCCAGAGGATCCGTGAAGAGGTGCACTATTCCAACCCAAGCTTGCCTCTGATGC ACCCTCCAGCCCACACCAAAGCCCCAGCTCAAGCCCCTGAGGCTACACCCACCTATGAAACAGGCCAGCG GAAGCGCCTCATTTCCTCCGTGGATGACTTCACAGAGTTTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231869 representing NM_001286718 Red=Cloning site Green=Tags(s) MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECGLNNRIRMIGQICDVAKT KKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNEDLHERL EVFKALTDNGRHITYLEEELAEFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIASMVHMICLLCIRTV SSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIM EDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYR KELQAVTWDILLDIIERLLQQLQNLDSPELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPE SSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVI SQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDLAMHSASLEDV KTAVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLG LPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLLQCLKQE SDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLERLRGTPEGFSRTDLHLAVVPVL TALISYHNYLDKTRQREMVYCLEQGLIYRCASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMA IPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVP YITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTE TCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGDLQGGSDS SSDPSTHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGVLDTSAPYSPGGSASLGPQTAVAA KPEKPPAGAQLPTAEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNA LMAAERFKEHRDTALYKSLSVPAAGTAKPPTLPRSNTDSAMVLEEGSPGETQVPVEPPELEDFEAALGTD RHCQRPDTYSRSSSASSQEEKSHLEELAAGGIPIERAISSEGARPAVDLSFQPSQPLSKSSSSPELQTLQ DILGDLGDKIDIGRLSPEAKVRSQSGILDGEAATWSATGEESRITVPPEGPLPSSSPRSPSGLRPRGYTI SDSAPSRRGKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESFERSVQLLD QIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQ FTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITP LDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLR HIKRLRQRIREEVHYSNPSLPLMHPPAHTKAPAQAPEATPTYETGQRKRLISSVDDFTEFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286718
ORF Size	5223 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286718.1, NP_001273647.1
RefSeq Size	6134 bp
RefSeq ORF	5226 bp
Locus ID	22084
MW	194.5 kDa

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