Syngap1 (NM_001281491) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gm1963; Syngap
Vector	pCMV6-Entry2
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231777 representing NM_001281491 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGGTCTCGAGCCTCCATCCATCGGGGGAGCATCCCCGCGATGTCCTATGCCCCCTTCAGAGATG TACGGGGACCCCCTATGCACCGAACCCAATACGTTCATTCCCCGTACGATCGTCCTGGCTGGAACCCTCG GTTCTGCATCATCTCGGGGAACCAGCTGCTCATGCTGGATGAGGATGAGATACACCCCCTTCTGATCCGC GACCGGAGGAGCGAGTCCAGCCGAAACAAACTGCTGAGACGCACCGTCTCTGTGCCAGTGGAGGGGCGGC CCCACGGCGAGCATGAATACCACTTGGGTCGCTCGAGGAGGAAGAGTGTCCCAGGGGGGAAACAGTACAG CATGGAGGCCGCCCCCGCTGCGCCCTTCCGGCCCTCGCAAGGCTTCCTGAGCCGGAGGCTAAAAAGCTCT ATCAAACGTACAAAGTCACAACCCAAACTTGACCGGACCAGCAGCTTTCGACAGATCCTGCCTCGCTTCC GAAGTGCTGACCATGACCGGGCCCGGCTGATGCAGAGCTTCAAGGAGTCACATTCCCACGAGTCCCTGCT GAGTCCCAGTAGTGCTGCTGAGGCCTTGGAGCTCAACCTGGATGAAGACTCCATTATCAAGCCAGTACAC AGCTCCATCCTGGGTCAGGAGTTCTGCTTTGAGGTAACAACATCATCTGGAACAAAATGCTTTGCCTGTC GGTCTGCAGCTGAAAGGGACAAATGGATTGAGAATCTGCAGAGGGCTGTAAAACCCAACAAGGACAACAG CCGCCGAGTAGATAACGTGCTGAAGCTATGGATCATAGAGGCTCGAGAGCTGCCCCCCAAGAAGAGATAT TACTGTGAGCTGTGCCTGGACGACATGCTGTATGCACGAACCACCTCCAAGCCCCGCTCGGCTTCAGGAG ACACCGTCTTTTGGGGCGAGCACTTTGAGTTTAACAACCTGCCTGCCGTCCGGGCCCTTCGGCTGCATCT GTACCGTGACTCAGACAAAAAGCGGAAGAAGGACAAGGCTGGCTACGTTGGCCTGGTGACTGTTCCAGTG GCCACCCTAGCTGGGCGCCACTTCACAGAGCAGTGGTACCCCGTGACCTTGCCGACAGGCAGTGGGGGCT CTGGGGGCATGGGCTCGGGGGGAGGAGGAGGGTCAGGGGGTGGCTCAGGGGGCAAAGGGAAAGGAGGTTG TCCTGCTGTGAGACTGAAAGCCCGTTACCAGACGATGAGCATCCTGCCCATGGAGCTGTATAAGGAGTTT GCAGAGTATGTGACCAACCATTACCGGATGCTATGTGCAGTGCTGGAGCCTGCCCTGAATGTCAAAGGCA AGGAAGAGGTGGCCAGTGCACTGGTTCACATCCTGCAGAGCACAGGCAAGGCCAAGGACTTCCTTTCAGA CATGGCCATGTCAGAGGTAGACCGATTCATGGAGCGGGAACACCTCATATTCCGAGAGAACACGCTCGCC ACTAAAGCCATAGAAGAGTATATGAGACTGATTGGCCAGAAATACCTCAAGGATGCCATTGGGGAGTTCA TTCGTGCTCTGTATGAATCTGAGGAGAACTGTGAAGTAGACCCCATCAAGTGCACAGCGTCCAGTCTGGC AGAGCACCAGGCCAACCTGCGGATGTGCTGTGAGTTGGCCCTGTGCAAGGTGGTCAACTCCCATTGCGTG TTCCCGAGGGAGCTGAAGGAGGTGTTTGCATCTTGGCGGCTGCGCTGCGCAGAGCGGGGCCGAGAGGACA TTGCTGACAGGCTGATCAGCGCCTCGCTCTTCCTGCGCTTCCTCTGCCCGGCCATTATGTCGCCCAGTCT ATTTGGGCTTATGCAGGAGTACCCAGATGAGCAGACCTCACGAACCCTCACCCTCATCGCCAAGGTCATC CAGAACCTGGCCAACTTTTCCAAGTTTACCTCAAAGGAGGACTTCCTGGGCTTCATGAATGAGTTTCTGG AGCTGGAGTGGGGCTCTATGCAGCAGTTCTTGTATGAGATATCCAACCTGGACACCTTGACCAACAGCAG CAGTTTTGAGGGCTATATAGACTTGGGCCGCGAGCTCTCCACACTTCACGCCCTGCTCTGGGAGGTGCTG CCCCAGCTCAGCAAGGAAGCCCTCCTGAAGCTGGGCCCACTGCCCCGGCTCCTCAATGACATCAGCACAG CCCTGAGGAACCCTAACATCCAAAGGCAGCCAAGCCGCCAGAGTGAACGGACTCGGTCTCAGCCCATGGT GCTGCGAGGGCCATCAGCCGAGATGCAGGGCTACATGATGCGGGACCTCAATAGCTCCATCGACCTTCAA TCCTTCATGGCTCGAGGCCTCAACAGCTCTATGGACATGGCTCGCCTCCCCTCCCCAACCAAGGAAAAAC CACCACCACCACCACCCGGTGGGGGTAAAGACCTTTTCTATGTGAGCCGGCCACCACTGGCCCGGTCCTC CCCAGCATACTGCACGAGCAGCTCGGACATCACAGAGCCAGAGCAGAAGATGCTGAGTGTCAACAAGAGT GTGTCCATGCTGGACCTTCAGGGCGACGGGCCTGGAGGTCGCCTTAACAGTAGCAGTGTTTCCAACCTGG CAGCTGTTGGGGACTTGTTGCATTCCAGCCAGGCCTCGCTGACAGCAGCTTTGGGGTTGCGGCCTGCACC TGCCGGGCGCCTCTCCCAGGGGAGTGGCTCTTCCATCACAGCAGCTGGCATGCGCCTCAGCCAGATGGGG GTCACTACAGATGGTGTCCCCGCCCAGCAACTGCGCATCCCCCTTTCCTTCCAGAACCCTCTCTTCCATA TGGCTGCTGATGGGCCAGGGCCCCCAGCAGGCCATGGAGGGAGCAGTGGTCATGGTCCACCTTCCTCCCA TCACCACCACCACCACCATCACCACCACCGAGGGGGAGAACCCCCAGGGGACACTTTTGCCCCATTCCAT GGCTATAGCAAGAGCGAGGACCTCTCTTCAGGGGTCCCTAAGCCCCCCGCCGCCTCCATCCTTCACAGCC ACAGCTACAGCGACGAGTTTGGACCCTCTGGCACTGATTTTACCCGCCGGCAGCTCTCGCTTCAGGACAG TCTACAGCACATGCTCTCCCCTCCCCAGATTACCATCGGTCCCCAGAGGCCAGCTCCCTCAGGGCCGGGA GGGGGCAGCGGCGGGGGCAGCGGTGGGGGCCAGCCACCCCCCTTGCAGAGGGGCAAGTCTCAACAGTTGA CAGTGAGTGCCGCCCAGAAACCTCGGCCGTCTAGCGGGAACCTGTTGCAGTCCCCGGAGCCAAGCTACGG ACCTGCCCGTCCTCGGCAGCAGAGCCTCAGCAAAGAGGGCAGCATTGGGGGCAGCGGGGGCAGCGGGGGC GGAGGGGGTGGGGGGCTCAAGCCCTCCATCACCAAGCAGCATTCCCAGACTCCATCCACGCTTAACCCCA CAATGCCAGCCTCGGAGCGGACCGTAGCCTGGGTCTCCAACATGCCTCACCTGTCGGCTGACATCGAGAG TGCACACATCGAGCGAGAAGAGTACAAGCTCAAGGAGTACTCCAAGTCCATGGACGAAAGCCGGCTGGAC AGGGTGAAGGAGTATGAGGAGGAGATACATTCGCTGAAGGAGAGGCTACACATGTCCAACCGGAAGCTGG AAGAGTATGAGCGGAGGTTGCTGTCCCAGGAAGAACAGACCAGCAAGATCCTGATGCAGTACCAAGCCCG CCTGGAGCAGAGTGAGAAGCGCTTGAGACAGCAGCAGGTGGAGAAGGACTCCCAGATCAAGAGCATCATT GGCAGGCTGATGCTGGTGGAGGAGGAGCTGCGTCGGGACCACCCCGCCATGGCTGAGCCGCTGCCTGAGC CCAAGAAGAGGCTGCTCGACGCTCAGGAGAGGCAGCTTCCCCCCTTGGGTCCAACAAACCCGCGTGTGAC GCTGGCCCCACCTTGGAACGGCCTGGCCCCCCCAGCCCCACCCCCCCCACCCCGGCTGCAGATCACAGAG AACGGCGAGTTCCGGAACACCGCAGACCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231777 representing NM_001281491 Red=Cloning site Green=Tags(s) MSRSRASIHRGSIPAMSYAPFRDVRGPPMHRTQYVHSPYDRPGWNPRFCIISGNQLLMLDEDEIHPLLIR DRRSESSRNKLLRRTVSVPVEGRPHGEHEYHLGRSRRKSVPGGKQYSMEAAPAAPFRPSQGFLSRRLKSS IKRTKSQPKLDRTSSFRQILPRFRSADHDRARLMQSFKESHSHESLLSPSSAAEALELNLDEDSIIKPVH SSILGQEFCFEVTTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRY YCELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKKRKKDKAGYVGLVTVPV ATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGKGKGGCPAVRLKARYQTMSILPMELYKEF AEYVTNHYRMLCAVLEPALNVKGKEEVASALVHILQSTGKAKDFLSDMAMSEVDRFMEREHLIFRENTLA TKAIEEYMRLIGQKYLKDAIGEFIRALYESEENCEVDPIKCTASSLAEHQANLRMCCELALCKVVNSHCV FPRELKEVFASWRLRCAERGREDIADRLISASLFLRFLCPAIMSPSLFGLMQEYPDEQTSRTLTLIAKVI QNLANFSKFTSKEDFLGFMNEFLELEWGSMQQFLYEISNLDTLTNSSSFEGYIDLGRELSTLHALLWEVL PQLSKEALLKLGPLPRLLNDISTALRNPNIQRQPSRQSERTRSQPMVLRGPSAEMQGYMMRDLNSSIDLQ SFMARGLNSSMDMARLPSPTKEKPPPPPPGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKS VSMLDLQGDGPGGRLNSSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLSQMG VTTDGVPAQQLRIPLSFQNPLFHMAADGPGPPAGHGGSSGHGPPSSHHHHHHHHHHRGGEPPGDTFAPFH GYSKSEDLSSGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQDSLQHMLSPPQITIGPQRPAPSGPG GGSGGGSGGGQPPPLQRGKSQQLTVSAAQKPRPSSGNLLQSPEPSYGPARPRQQSLSKEGSIGGSGGSGG GGGGGLKPSITKQHSQTPSTLNPTMPASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDESRLD RVKEYEEEIHSLKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQVEKDSQIKSII GRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQERQLPPLGPTNPRVTLAPPWNGLAPPAPPPPPRLQITE NGEFRNTADH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NM_001281491
ORF Size	4020 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281491.2
RefSeq Size	4023 bp
RefSeq ORF	4023 bp
Locus ID	240057
UniProt ID	F6SEU4
MW	148.7 kDa
Gene Summary	This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.