Zmynd8 (NM_001291158) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110013E22Rik; 2010005I16Rik; 3632413B07Rik; AI316811; AL024039; Prkcbp1; RACK7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231711 representing NM_001291158 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATCTCTACTCGCTCCAAAGAACCTAAGGAAGATCCTGTCTCTACAGAGAAAACGGCCCCGAAAC GGAGGTTCCCCAGCCCTCCACATTCCTCCAATGGCCATTCGCCCCAAGACTCATCCACGAGCCCCATTAA AAAGAAAAAGAAACCCGGCTTACTCAACAGTAGCAATAAGGAACAGTCAGAGCTAAGACATGGTCCGTTT TACTATATGAAGCAGCCACTCACCACAGACCCTGTTGATGTTGTACCGCAGGACGGACGGAATGACTTCT ATTGCTGGGTTTGTCACCGGGAAGGACAAGTCCTTTGCTGTGAGCTCTGTCCCCGGGTTTATCACGCTAA GTGTCTGAGACTGACATCGGAGCCAGAGGGGGACTGGTTTTGTCCTGAATGTGAGAAGATTACAGTAGCA GAATGCATCGAGACGCAGAGCAAAGCCATGACCATGCTGACCATTGAACAACTGTCCTACCTGCTCAAGT TTGCCATTCAGAAAATGAAGCAGCCAGGGACGGATGCATTCCAGAAGCCTGTTCCATTGGAGCAACACCC TGACTATGCAGAATATATTTTCCACCCCATGGACCTTTGTACATTGGAAAAGAATGCAAAAAAGAAGATG TACGGCTGCACAGAAGCCTTCCTGGCCGATGCCAAGTGGATCCTGCACAACTGCATTATTTATAATGGGG GAAATCACAAGTTGACGCAAATAGCAAAAGTCGTCATCAAAATCTGTGAGCACGAGATGAATGAAATCGA AGTCTGTCCAGAATGTTATCTTGCAGCTTGCCAAAAACGAGACAACTGGTTCTGTGAGCCCTGTAGCAAT CCGCACCCTTTGGTCTGGGCAAAACTGAAAGGATTTCCATTCTGGCCAGCGAAAGCTCTGAGGGACAAAG ACGGGCAGGTTGACGCCCGTTTCTTTGGACAACATGACAGAGCCTGGGTTCCAGTCAATAATTGCTACCT CATGTCTAAAGAAATCCCCTTTTCTGTGAAAAAGACTAAAAGTATCTTCAACAGCGCCATGCAAGAGATG GAAGTTTACGTGGAGAACATACGGAGGAAGTTTGGGGTTTTTAATTACTCCCCGTTCAGGACGCCCTACA CGCCCAACAACCAGTACCAAATGCTGCTGGATCCCAGCAACCCCAGCGCGGGCACAGCCAAGACAGACAA ACAGGAGAAGGTGAAGCTTAATTTTGACATGACAGCGTCCCCCAAGATCCTTCTGAGCAAGCCCCTTCTG AGCGGGGGTGCCGGCCGCAGGATCTCCCTGTCCGACATGCCTCGCTCCCCCACCAGTACGAACTCTTCCG TGCACACGGGCTCCGATGTGGAGCAGGACCCCGAGAAGAAGGCCCCGTCCAGCCACTTCAGCGCAAGCGA GGAGTCCATGGACTTCCTTGATAAGAGCACAGCTTCTCCAGCCTCCACCAAGACGGGGCAAGCCGGGAGC TTGTCTGGCAGCCCAAAGCCTTTCTCTCCGCAAGCGCCGACACCCATCATGACAAAACCCGACAAGACTT CCACCTCCACCACCGGGAGCATCCTGAACCTGAACTTGGATCGAAGCAAGGCCGAGATGGACCTGAAGGA GCTGAGCGAGTCGGTCCAGCAGCAGTCAGCCCCCGTCCCTCTCATCTCTCCCAAGCGGCAGATTCGAAGC CGGTTCCAGCTCAACCTGGACAAGACCATAGAGAGTTGCAAAGCACAGCTAGGCATAAATGAGATCTCAG AGGATGTTTATACAGCCGTGGAGCACAGCGATTCCGAGGACTCCGAAAAGTCGGAGAGCAGCGACAGCGA GTACGTCAGCGATGAGGAACAGAAGCCCAAGAATGAGCCCGAGGACCCCGAGGACAAAGAGGGGAGTCGG GTGGACAAAGAGGCCCCTGCCATCAAAAGGAAGCCCAAACCCACAAACCAGGTAGAGGTCAAAGAGGAAG CGAAGAGCAACTCTCCTGTCAGCGAGAAGCCGGACCCCACACCCGCCAAGGACAAGGCCAGCCCAGAGCC TGAGAAGGACTTTGTAGAGAAAGCAAAGCCATCACCTCATCCCACAAAGGACAAACTGAAAGGAAAGGAT GAAACGGATTCTCCCACAGTGCACTTGGGCTTGGATTCGGACTCGGAGAGCGAACTTGTCATAGACTTAG GAGAGGATCCTTCTGGGAGGGAGGGTCGAAAAAACAAGAAAGATCCCAAGGTGCCGTCGCCTAAGCAAGA CGCTATAGGTAAACCGCCACCGTCGTCCACTTCGGCGGGCAACCAGTCTCCCCCAGAGACACCGGTACTC ACCCGCTCAGCCACCCAAGCACCCGCGGCTGGGGTCACCGTGGCCGCCGCCACCACCAGCACGATGTCTA CCGTCACAGTCACGGCACCGGCCACCGCCGTCACGGGAAGCCCGGTGAAGAAGCAGAGGCCGCTCTTACC GAAGGAGACTGTCCCAGCTGTGCAGCGGGTCGTGTGGAACGCATCAAGTAAGTTTCAAACGTCCTCCCAA AAGTGGCACATGCAGAAGATACAGCGCCAGCAGCAGCAGCAGCAACAACAACAGCAGAGCCAACAGCAGA GCCAGCAGCAGCAGCCTCAGTCTTCCCAGGGGACGAGATATCAGACCAGACAGGCTGTGAAAGCTGTCCA GCAGAAGGAGGTCACCCAGAGCCCATCCACGTCCACCATCACGCTGGTGACCAGCACACAGCCGGCAGCC CTGGTCAGCAGTTCGGGCTCAGCAAGCACCCTGGCGTCTGCAATCAATGCCGACCTTCCCATTGCCACCG CCTCGGCCGACGTGGCCGCAGACATTGCCAAGTACACCAGCAAAATGATGGATGCCATAAAGGGGACGAT GACAGAAATCTACAATGACCTCTCCAAGAACACCACTGGGAGCACAATAGCTGAGATTCGAAGGCTGAGG ATTGAGATTGAGAAACTGCAGTGGCTGCACCAGCAGGAGCTCGCTGAGATGAAGCACAACCTGGAGTTGA CCATGGCCGAGATGCGGCAGAGCCTGGAACAGGAGCGGGATCGGCTCATTGCCGAGGTGAAAAAGCAACT GGAGCTGGAGAAGCAGCAGGCGGTGGACGAGACCAAGAAGAAGCAGTGGTGTGCCAACTGCAAGAAGGAG GCCATTTTCTACTGCTGCTGGAACACCAGCTACTGTGACTACCCCTGTCAGCAGGCCCACTGGCCCGAGC ACATGAAGTCCTGTACCCAGTCGGCGACTGCCCCTCAGCAGGAAGCAGATGCCGAGGCAAGCACAGAAAC AGGAAATAAGTCATCGCAGGGCAACTCCTCCAACACACAGTCAGCACCTTCAGAACCGGCCAGCGCCCCC AAAGAGAAAGAGGCGCCAGCGGAGAAGAGCAAGGACAGTAGTAACTCGACCCTGGATCTTTCCGGCTCCA GAGAGACGCCCTCCTCTATGCTCTTAGGCTCCAATCAAAGCTCTGTTAGCAAGAGGTGTGACAAGCAGCC TGCCTATACCCCAACCACTACAGACCACCAGCCGCACCCCAACTACCCAGCCCAGAAGTACCATTCCCGG AGCAGCAAGGCAGGTTTGTGGAGCAGCAGCGAGGAGAAGCGAGCGTCATCCCGCTCTGAGCACAGTGGAG GGACCAGCACGAAGAACCTCATGCCCAAAGAGTCCCGGGAGTCTCGGCTAGATGCCTTCTGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231711 representing NM_001291158 Red=Cloning site Green=Tags(s) MDISTRSKEPKEDPVSTEKTAPKRRFPSPPHSSNGHSPQDSSTSPIKKKKKPGLLNSSNKEQSELRHGPF YYMKQPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLTSEPEGDWFCPECEKITVA ECIETQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAEYIFHPMDLCTLEKNAKKKM YGCTEAFLADAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCPECYLAACQKRDNWFCEPCSN PHPLVWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPVNNCYLMSKEIPFSVKKTKSIFNSAMQEM EVYVENIRRKFGVFNYSPFRTPYTPNNQYQMLLDPSNPSAGTAKTDKQEKVKLNFDMTASPKILLSKPLL SGGAGRRISLSDMPRSPTSTNSSVHTGSDVEQDPEKKAPSSHFSASEESMDFLDKSTASPASTKTGQAGS LSGSPKPFSPQAPTPIMTKPDKTSTSTTGSILNLNLDRSKAEMDLKELSESVQQQSAPVPLISPKRQIRS RFQLNLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSESSDSEYVSDEEQKPKNEPEDPEDKEGSR VDKEAPAIKRKPKPTNQVEVKEEAKSNSPVSEKPDPTPAKDKASPEPEKDFVEKAKPSPHPTKDKLKGKD ETDSPTVHLGLDSDSESELVIDLGEDPSGREGRKNKKDPKVPSPKQDAIGKPPPSSTSAGNQSPPETPVL TRSATQAPAAGVTVAAATTSTMSTVTVTAPATAVTGSPVKKQRPLLPKETVPAVQRVVWNASSKFQTSSQ KWHMQKIQRQQQQQQQQQQSQQQSQQQQPQSSQGTRYQTRQAVKAVQQKEVTQSPSTSTITLVTSTQPAA LVSSSGSASTLASAINADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLR IEIEKLQWLHQQELAEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKE AIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEASTETGNKSSQGNSSNTQSAPSEPASAP KEKEAPAEKSKDSSNSTLDLSGSRETPSSMLLGSNQSSVSKRCDKQPAYTPTTTDHQPHPNYPAQKYHSR SSKAGLWSSSEEKRASSRSEHSGGTSTKNLMPKESRESRLDAFWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291158
ORF Size	3705 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291158.1, NP_001278087.1
RefSeq Size	7345 bp
RefSeq ORF	3708 bp
Locus ID	228880
MW	137.4 kDa

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