Grip1 (NM_001277293) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4931400F03Rik; eb; GRIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231590 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGATGAGAGTCCCT ACACTAAATCTGCCAGCCAGACAAAGCCGCCCGATGGAGCATTGGCTGTGAGGAGACAGAGCATCCCAGA GGAATTCAAGGGCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCG GGAGGCATTGATAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTG ACCAGCTGGATGTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGA GATCATCAGCCTGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTC TCTGTACAAGGATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTG GTTTTGTCATCCGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCG TCCTGGAGGGCCTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATT CGGCTCCTGGGAACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGC TGATAGAATACGATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGC CAAAACTCCAGGTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTC ATAGACAAAATCAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCT CCATTGACGGCACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGA CCAGGTCAAGCTGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGTGAAA ATTCAGAGGAGCGACAGACAACATCCCTGGGATGCCTGGGCCAGCAACCAGTGCGGCGTTCATACCAACC ATCACCATAACACGTACCACCCAGACCATTGCAGAGTGCCAGCCCTGACTTTCCCGAAAGCGCTTCCTCC AAACAGCCCTCCGGCTATGGTGCCCTCATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTG AACATGGGGACTTTACCTCGAAGCCTCTACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGA AAAAGAAAGACTTCAAAAGCTCACTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCA CACTGAAACCACAGAGGTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGC GTGTTTGCCACAGAGACGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGA GATGTGGTGTGCTACAGATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTT CGAGGAAGCCAATCAACTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGAT GTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAAC TTGGAATAACCATCAGTTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAA AGGCAGTGTGGCACACAGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGG CTGGATAACTGTTCCATGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAA TCCGCAAAGATGAAGATAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAA GCGCTATGGGGGGCCCCTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCG AGCCTCACTAAAGGGGGATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCA ATAGCAGCAGCTTGAAGGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGT CACCCTGAAAATTAAGAAACAGACAGATGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGC CACTCGGGGGACCTAGGAGATGGTGAGGAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATG CGTACCCCTCCACGGTGCCCAGCGTGGACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAG CTATGGGAGTCAAGGCTCAACTTTTCAGACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCA AAGCAAAGAACCAGCCTGTCCCCAGTCCCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTA ATGAAGACTGGGATCGATCCACAGCCAGTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGA GGAAAACTTCTGGTCTCAAGCATTGGAGGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTT GAGGCAACAATCATGTCGGGGAGTACTATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGG GGCGACAGGCCAGCTTCCAGGAACGGAGCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACAC CCTGCCCTCAGACGTGGGCAGAAAGTCTGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATG TCCCCAACTCCGGTGGAGCTACACAAGGTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCA GTGTGGCAGATGGCCTGCTGGAGAAAGGCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGT TGGGGGCTTGAAGCCCTACGACAGGCTCTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGC CTGGTGGTGCCTCTCATAGCTGAATCTGGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCT CCCAGAAGTCGATAGAACAGCCGGCTCTGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCA ACCCAGCCACGGGATTCCTGGAGATACTGTATACTTTTGGCAAAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231590 protein sequence Red=Cloning site Green=Tags(s) MPGWKKNIPICLQAEEQERDESPYTKSASQTKPPDGALAVRRQSIPEEFKGSTVVELMKKEGTTLGLTVS GGIDKDGKPRVSNLRQGGIAARSDQLDVGDYIKAVNGINLAKFRHDEIISLLKNVGERVVLEVEYELPPV SVQGSSVMFRTVEVTLHKEGNTFGFVIRGGAHDDRNKSRPVVITCVRPGGPADREGTIKPGDRLLSVDGI RLLGTTHAEAMSILKQCGQEATLLIEYDVSVMDSVATASGPLLVEVAKTPGASLGVALTTSVCCNKQVIV IDKIKSASIADRCGALHVGDHILSIDGTSMEYCTLAEATQFLANTTDQVKLEILPHHQTRLALKGPDHVK IQRSDRQHPWDAWASNQCGVHTNHHHNTYHPDHCRVPALTFPKALPPNSPPAMVPSSSPTSMSAYSLSSL NMGTLPRSLYSTSPRGTMMRRRLKKKDFKSSLSLASSTVGLAGQVVHTETTEVVLTADPVTGFGIQLQGS VFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQLLRDSSITSKVTLEIEFD VAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKLLAIDNIR LDNCSMEDAVQILQQCEDLVKLKIRKDEDNSDEQESSGAIIYTVELKRYGGPLGITISGTEEPFDPIIIS SLTKGGLAERTGAIHIGDRILAINSSSLKGKPLSEAIHLLQMAGETVTLKIKKQTDAQSASSPKKFPIPG HSGDLGDGEEDPSPIQKPGKLSDAYPSTVPSVDSAVDSWDGSGIDASYGSQGSTFQTSGYNYNTYDWRSP KQRTSLSPVPKPRSQTYPDVGLSNEDWDRSTASGFVGASDSADAEQEENFWSQALEDLETCGQSGILREL EATIMSGSTMSLNHEAPMARSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIM SPTPVELHKVTLYKDSGMEDFGFSVADGLLEKGVYVKNIRPAGPGDVGGLKPYDRLLQVNHVRTRDFDCC LVVPLIAESGNKLDLVISRNPLASQKSIEQPALPSDWSEQNSAFFQQPSHGIPGDTVYFWQSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277293
ORF Size	3342 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277293.1, NP_001264222.1
RefSeq Size	3959 bp
RefSeq ORF	3342 bp
Locus ID	74053
MW	120.5 kDa
Gene Summary	This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

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