Uspl1 (NM_001286824) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	E430026A01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231553 representing NM_001286824 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGATGATTCTCTGAAGATTGGAAATGGTTTGCCACTGGTTGGACCAGGGACTGATAATTATGCTT CAGCTAAAGTCACAACAGACGGGCATTGCCCTGCTTGTAGAGCGAAAGGCAAGCTAAGTGCCTTAAAACC TTACCGCATTAGCTTCCAAGAATCTGTCTTTCTGTGTGAGGATCTTCAGTGCATTTATCCTTTGGGCTCT GAGTCACTTACTAATTTAATTTCTCCTGATTCAGAAGATTGTCCCACTCCAAGTAAGCCTCAAAAACGAA AAAGATTGGAAACCAACTGTAGGAATTCACCTCTTCCAGTACATTCTAAAAAGACTAGAAGTCACATTGT CACTGATAGTGAACCAATTGTGAATGGTAAATATAATGGAGAAGTGTGTGATGACTTCTCAGCAAGCTTC CCAGATACCAGTGCTCACCAGGATCCTGCCAGCACAGCTGCTTCTGTGGAGCAGAGTGAGGCTTTGGAGG CTGATGATGTGGTTGTGGCCGCTACAGAAGATCCTGCTACAGTTAGTGTCACATCTGAGCTGGAAATGCC AGCTAAGAGCAGATGTTTACCACTTTGCCAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCCTGTGC TGGTTAGACTGTATCCTGTCAGCCCTGGTGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGCGTGCT CCAGGGAGGAGTGTGTATTTGGAAGGCTGTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCACACCCA TCACCTGCATGGCGTCACAGGTGAAGATTGTAAAAAATTGACATCAGAAATATTTACAGAAATAGATACC TGTTTGAATAAAGTTAGAGATGAAATTTTTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGGTGACA TGGAAAGTCCTGTGTTTGCACTTCCTGTACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTTACATA TTCTTTTTCTTGGAATTTTGAATGTTCCCATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGAGTCTG GTCACCTTTACCAATATTGTTCCTGAGTGGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAACAGCT GCAACAGTAAATCACAAATAAGAAAAATGGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCACTTCGT GGAGGGCTTACCAAGGAGGGATTTGCAACACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAGTCACG TCGGTAATACAGTACCAAGCAAATAACCATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTGGCTGG AGTGTGATGACCTAAAGGGTCCCTGTGCTAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAGACACA TATCGTTATTTGGGAAAGAAAATCCCAGGTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGAAGCCA AATGTGCAGCCTGTATCAGGTGAGGAGCAGCCGACTTGTCCAGCACTGTGTTCTCTGGCTGGGACCGCCA CCTCAGAACCCTCAGTCGCCCATCCCACATCTATGGCAGGCGCTCCTCAGACTCTTCCAGAAATCCAAGC TGTAGCTCATGGAGATAGTGTACTTTCAGGAGCAAAAGGCATGGTCGACAGCATTTTACCCTCAGCACTT GAAGAAACCATCCAGGAAACTGCCTCTGTTTCTCAGGTTGACTCCAAAGATTGCCTGTTGGAAGACAAAC CCGTGGCAGGAAGTGCAGCACTGGTCAGAGTACTTGCTTTTCAGCCACAGGACTCCCCGGGGTCTTCGGG GTCTTCCTTAGTGTCCAGCCTGTGTGAAGGAAAGCTAGTTGCCCCATGTGTGGATTCAAGTTTCCCATCC CAAGCTGTGAGCACAGACCTGCAGGCAGTACTGTCCCAGGCAGGGGACACTGTGGTTCCTAACCCTGTGA CTGATGCCCCTGTTCCTGTTCTTGTTCAGGAATTGAAGTCCCTGGCAACTGAGAAGGACTCTCAGACACA GTTGCTGCCACTGAAAACTGAGAAGTTAGATCCTGAGCAACCTGGTAAATCTCAGGCATCTAATTTGAGA AAACGAGAAACCACAGCGTCTTCTAAAACAGTAGCAGCCAGGTCAGCACAGAATCAGCCTCGGAAAGAAG ATCAGAAGAGAGCCTTCGTGGGAAGTTGGGTGAAGGGGCTATTGAGCAGGGGTGGTGCTTTTATGCCAAC CTGTGTTTTGTCTCAGAGTAGAGCAGTAAGTGACCTGCAACCTTCTGTTAAAGGAGCCAGTAATTTTGAT GGCTTTAAAACGAAAAGTATAAGCCGCAGGTCTAAAAGAATGTCCAGGAAAGCTAAGCACATGGAGGAAC TGTCTCCAAGGAACAGCTCTCCACCATTGAGCTGGACAGCTGCTCTGACCCAGGCTGCTGAGAACGCCAC CTCAGCTCTGTTGAGGGAGCAGGAAGGCTCACGTCCAGCTCCCCTCAGGCACAGATCTCCTGGCAATGAA AGCGCCATCTCTCCAGCAAGCCGTGGAGATGCGGCTGAAGATCAGGTTCATAAGCTTCGGCTCAAACTTC TTAAAAAATTAAAGGCAAAAAAGAAGAAACTAGCTGCCCTCATTTCTTCCCCGCACCGTGAACCATCTCT AAGTGACCACTCAGAGCCTGCGTCCCATTGTGGAACACCTGCAAGTGACCAGTCAGAGCCTGTATCCCAT TGTGGATCTCCAAATGACTGTGAGTCCATAGAAGATCTGTTAAAAGAGCTACAGCATCAAATTGACCTTG CAGACAGCAAATCTGGGTGCACCTCTGCTCCTGATGCCACCTCAAATAACAGCCAGAGTCATGAGGAGAT TTTAGCAGAGTTGCTGTCGCCTACAGCCATGTCAGAGCCCTCAGAAAGTGGGGAGCTTGAGTTGAGGTAC TTGGAAATGGGGGACAGCACCCCAGCACAAGCACCTAGTGAATTCAGTGTTGTCTCCCAGAACACATGTC TGAAACAGGACCATGATTACTGCAGCCCTGAGAAAGGCCAGCGGGAAGTTGATCTGCATTCAGTCATGGA CAGTGCCTGTATAAGAACCTTGAACTTGGGGAGTCCCATGAAGACTGATATCTTTGATGATTTTTTTTCC ACCTCAGCACTGAATTCTTTAACAAACGACACATTAGACATACCTCATTTTGATGACTCTCTGTTTGAGA ATTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231553 representing NM_001286824 Red=Cloning site Green=Tags(s) MTDDSLKIGNGLPLVGPGTDNYASAKVTTDGHCPACRAKGKLSALKPYRISFQESVFLCEDLQCIYPLGS ESLTNLISPDSEDCPTPSKPQKRKRLETNCRNSPLPVHSKKTRSHIVTDSEPIVNGKYNGEVCDDFSASF PDTSAHQDPASTAASVEQSEALEADDVVVAATEDPATVSVTSELEMPAKSRCLPLCQTLCVQWKNTQALC WLDCILSALVHLEVLRKTVLEACSREECVFGRLFEMYHQADELLHTHHLHGVTGEDCKKLTSEIFTEIDT CLNKVRDEIFAKLQPKLRCTLGDMESPVFALPVLLKLEPHVESLFTYSFSWNFECSHCGHQYQNRCVKSL VTFTNIVPEWHPLNAAHFGPCNSCNSKSQIRKMVLERASPILMLHFVEGLPRRDLQHYAFHFEGSLYQVT SVIQYQANNHFITWFLDADGSWLECDDLKGPCAKRHVTCEVPASETHIVIWERKSQVPIEEAACLPCMKP NVQPVSGEEQPTCPALCSLAGTATSEPSVAHPTSMAGAPQTLPEIQAVAHGDSVLSGAKGMVDSILPSAL EETIQETASVSQVDSKDCLLEDKPVAGSAALVRVLAFQPQDSPGSSGSSLVSSLCEGKLVAPCVDSSFPS QAVSTDLQAVLSQAGDTVVPNPVTDAPVPVLVQELKSLATEKDSQTQLLPLKTEKLDPEQPGKSQASNLR KRETTASSKTVAARSAQNQPRKEDQKRAFVGSWVKGLLSRGGAFMPTCVLSQSRAVSDLQPSVKGASNFD GFKTKSISRRSKRMSRKAKHMEELSPRNSSPPLSWTAALTQAAENATSALLREQEGSRPAPLRHRSPGNE SAISPASRGDAAEDQVHKLRLKLLKKLKAKKKKLAALISSPHREPSLSDHSEPASHCGTPASDQSEPVSH CGSPNDCESIEDLLKELQHQIDLADSKSGCTSAPDATSNNSQSHEEILAELLSPTAMSEPSESGELELRY LEMGDSTPAQAPSEFSVVSQNTCLKQDHDYCSPEKGQREVDLHSVMDSACIRTLNLGSPMKTDIFDDFFS TSALNSLTNDTLDIPHFDDSLFENC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286824
ORF Size	3225 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286824.1, NP_001273753.1
RefSeq Size	3730 bp
RefSeq ORF	3228 bp
Locus ID	231915
UniProt ID	Q3ULM6
MW	117.4 kDa
Gene Summary	SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function]

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