Slc8a1 (NM_001286684) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI852629; AV344025; D930008O12Rik; Ncx1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231402 representing NM_001286684 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGATTAAGTCTCCCACCCAATGTTTCAATGGGATTTCGTCTGGTAGCTCTGGTGGCTCTCTTGT TTTCCCATGTTGACCATATAACTGCAGATACAGAGGCAGAAACAGGAGGAAATGAAACCACTGAATGTAC TGGCTCATATTACTGTAAGAAAGGGGTGATCTTGCCCATTTGGGAACCCCAAGACCCATCTTTTGGGGAC AAAATTGCTAGAGCAACTGTGTATTTTGTGGCCATGGTCTACATGTTCCTTGGAGTTTCTATTATTGCAG ACCGGTTTATGTCCTCTATAGAGGTCATCACCTCTCAAGAGAAAGAAATAACGATAAAGAAACCGAATGG AGAGACCACCAAGACGACGGTGAGAATCTGGAACGAGACTGTGTCGAACCTGACCTTGATGGCCCTGGGA TCTTCTGCTCCTGAGATTCTCCTGTCAGTCATTGAAGTGTGCGGCCATAACTTCACCGCAGGGGACCTGG GTCCCAGCACCATCGTGGGAAGTGCTGCCTTTAACATGTTCATCATAATCGCACTCTGTGTTTACGTGGT CCCTGATGGAGAGACAAGGAAGATCAAGCATCTGCGTGTGTTCTTTGTGACAGCAGCCTGGAGCATCTTT GCCTATACCTGGCTTTATATAATCTTGTCTGTCAGCTCTCCTGGAGTTGTGGAGGTCTGGGAAGGCTTGC TTACTTTCTTCTTCTTTCCCATCTGCGTTGTGTTCGCGTGGGTAGCAGACAGGCGGCTTCTCTTTTACAA GTATGTCTACAAGCGGTACAGGGCCGGCAAGCAGAGGGGGATGATCATTGAACATGAAGGAGACAGACCA GCTTCCAAAACTGAAATCGAAATGGATGGGAAAGTGGTCAACTCTCATGTTGACAATTTCTTAGATGGGG CTCTGGTTTTGGAAGTTGATGAGAGGGACCAAGATGATGAGGAAGCCAGGCGTGAGATGGCAAGGATTCT GAAGGAACTTAAGCAGAAGCATCCTGAGAAAGAAATTGAGCAATTAATAGAATTAGCCAACTACCAGGTC CTAAGTCAACAGCAGAAAAGCCGAGCATTTTACAGGATTCAAGCTACTCGCCTGATGACCGGAGCTGGCA ACATCTTGAAGAGGCACGCAGCTGATCAAGCAAGGAAGGCTGTCAGTATGCATGAAGTCAACATGGAAAT GGCTGAAAACGACCCAGTCAGTAAGATCTTCTTTGAGCAAGGAACATACCAGTGTCTAGAGAACTGTGGT ACTGTGGCCCTCACCATTATGCGCAGAGGGGGCGACTTGAGCACCACTGTGTTTGTTGACTTCAGGACAG AAGACGGCACAGCCAATGCTGGGTCTGATTATGAATTCACGGAAGGGACTGTGATCTTCAAACCAGGGGA GACCCAGAAGGAAATCAGAGTTGGCATCATTGATGATGATATCTTTGAAGAAGATGAAAACTTCCTTGTG CATCTTAGCAATGTCAGAGTCTCTTCAGATGTTTCAGAAGATGGCATACTAGAATCCAATCACGCTTCTT CAATTGCTTGTCTTGGGTCACCCAGCACTGCCACCATAACCATTTTTGATGATGACCATGCAGGCATCTT TACATTTGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTTTTGAGAACC TCTGGAGCTCGAGGAAATGTTATCATTCCCTACAAAACTATTGAAGGCACAGCCCGAGGTGGAGGGGAAG ACTTTGAGGACACCTGTGGAGAGCTCGAATTCCAGAACGATGAAATAGTCAAAACAATATCAGTCAAGGT AATCGATGACGAGGAGTATGAGAAAAACAAGACCTTCTTCATTGAGATTGGAGAGCCCCGTCTGGTGGAG ATGAGTGAGAAGAAAGCCCTGTTGTTGAATGAGCTTGGTGGCTTCACATTAACAGGAAAAGAGATGTATG GCCAACCTATCTTCAGGAAGGTCCATGCTAGAGATCATCCGATTCCCTCTACTGTAATCACCATCTCAGA GGAATATGATGACAAGCAGCCACTGACCAGCAAAGAAGAGGAGGAGAGGCGCATTGCGGAAATGGGGCGC CCCATCCTAGGCGAGCACACCAAGCTGGAGGTGATCATCGAAGAGTCTTACGAATTCAAGAGCACTGTGG ACAAACTCATTAAGAAGACGAACCTGGCCCTTGTGGTGGGGACCAACAGCTGGAGAGAGCAGTTTATCGA AGCCATCACTGTCAGCGCTGGGGAAGATGACGATGATGATGAATGTGGGGAGGAGAAGCTGCCCTCCTGT TTTGATTACGTGATGCACTTTCTCACAGTGTTCTGGAAGGTTCTGTTTGCCTTCGTCCCACCTACAGAAT ACTGGAATGGCTGGGCCTGCTTCATTGTCTCCATCCTCATGATCGGCCTACTGACCGCCTTCATTGGAGA CCTGGCTTCCCACTTTGGCTGCACCATTGGTCTGAAAGATTCCGTGACTGCCGTTGTGTTTGTTGCTCTT GGAACCTCGGTGCCAGACACATTTGCCAGCAAAGTAGCAGCTACCCAGGACCAGTATGCAGATGCGTCTA TAGGCAATGTCACTGGAAGCAATGCTGTGAATGTCTTCCTGGGAATCGGCGTGGCCTGGTCCATTGCTGC CATCTACCATGCGGCCAACGGGGAACAGTTCAAAGTGTCCCCCGGCACGCTAGCTTTCTCTGTCACTCTC TTCACTATTTTTGCTTTCATCAACGTGGGGGTGCTGCTGTATCGGCGGAGGCCAGAAATAGGAGCCATCT TCGGAATGTCAATAATTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231402 representing NM_001286684 Red=Cloning site Green=Tags(s) MLRLSLPPNVSMGFRLVALVALLFSHVDHITADTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGD KIARATVYFVAMVYMFLGVSIIADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSNLTLMALG SSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFIIIALCVYVVPDGETRKIKHLRVFFVTAAWSIF AYTWLYIILSVSSPGVVEVWEGLLTFFFFPICVVFAWVADRRLLFYKYVYKRYRAGKQRGMIIEHEGDRP ASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPEKEIEQLIELANYQV LSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMEMAENDPVSKIFFEQGTYQCLENCG TVALTIMRRGGDLSTTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLV HLSNVRVSSDVSEDGILESNHASSIACLGSPSTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRT SGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIGEPRLVE MSEKKALLLNELGGFTLTGKEMYGQPIFRKVHARDHPIPSTVITISEEYDDKQPLTSKEEEERRIAEMGR PILGEHTKLEVIIEESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSC FDYVMHFLTVFWKVLFAFVPPTEYWNGWACFIVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVAL GTSVPDTFASKVAATQDQYADASIGNVTGSNAVNVFLGIGVAWSIAAIYHAANGEQFKVSPGTLAFSVTL FTIFAFINVGVLLYRRRPEIGAIFGMSIIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286684
ORF Size	2820 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286684.1, NP_001273613.1
RefSeq Size	15847 bp
RefSeq ORF	2823 bp
Locus ID	20541
MW	105.1 kDa
Gene Summary	Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:8659820). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:10967099). Required for normal embryonic heart development and the onset of heart contractions (PubMed:10967099).[UniProtKB/Swiss-Prot Function]

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