Uspl1 (NM_001286825) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	E430026A01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231322 representing NM_001286825 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTAAGAGCAGATGTTTACCACTTTGCCAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCC TGTGCTGGTTAGACTGTATCCTGTCAGCCCTGGTGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGC GTGCTCCAGGGAGGAGTGTGTATTTGGAAGGCTGTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCAC ACCCATCACCTGCATGGCGTCACAGGTGAAGATTGTAAAAAATTGACATCAGAAATATTTACAGAAATAG ATACCTGTTTGAATAAAGTTAGAGATGAAATTTTTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGG TGACATGGAAAGTCCTGTGTTTGCACTTCCTGTACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTT ACATATTCTTTTTCTTGGAATTTTGAATGTTCCCATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGA GTCTGGTCACCTTTACCAATATTGTTCCTGAGTGGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAA CAGCTGCAACAGTAAATCACAAATAAGAAAAATGGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCAC TTCGTGGAGGGCTTACCAAGGAGGGATTTGCAACACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAG TCACGTCGGTAATACAGTACCAAGCAAATAACCATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTG GCTGGAGTGTGATGACCTAAAGGGTCCCTGTGCTAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAG ACACATATCGTTATTTGGGAAAGAAAATCCCAGGTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGA AGCCAAATGTGCAGCCTGTATCAGGTGAGGAGCAGCCGACTTGTCCAGCACTGTGTTCTCTGGCTGGGAC CGCCACCTCAGAACCCTCAGTCGCCCATCCCACATCTATGGCAGGCGCTCCTCAGACTCTTCCAGAAATC CAAGCTGTAGCTCATGGAGATAGTGTACTTTCAGGAGCAAAAGGCATGGTCGACAGCATTTTACCCTCAG CACTTGAAGAAACCATCCAGGAAACTGCCTCTGTTTCTCAGGTTGACTCCAAAGATTGCCTGTTGGAAGA CAAACCCGTGGCAGGAAGTGCAGCACTGGTCAGAGTACTTGCTTTTCAGCCACAGGACTCCCCGGGGTCT TCGGGGTCTTCCTTAGTGTCCAGCCTGTGTGAAGGAAAGCTAGTTGCCCCATGTGTGGATTCAAGTTTCC CATCCCAAGCTGTGAGCACAGACCTGCAGGCAGTACTGTCCCAGGCAGGGGACACTGTGGTTCCTAACCC TGTGACTGATGCCCCTGTTCCTGTTCTTGTTCAGGAATTGAAGTCCCTGGCAACTGAGAAGGACTCTCAG ACACAGTTGCTGCCACTGAAAACTGAGAAGTTAGATCCTGAGCAACCTGGTAAATCTCAGGCATCTAATT TGAGAAAACGAGAAACCACAGCGTCTTCTAAAACAGTAGCAGCCAGGTCAGCACAGAATCAGCCTCGGAA AGAAGATCAGAAGAGAGCCTTCGTGGGAAGTTGGGTGAAGGGGCTATTGAGCAGGGGTGGTGCTTTTATG CCAACCTGTGTTTTGTCTCAGAGTAGAGCAGTAAGTGACCTGCAACCTTCTGTTAAAGGAGCCAGTAATT TTGATGGCTTTAAAACGAAAAGTATAAGCCGCAGGTCTAAAAGAATGTCCAGGAAAGCTAAGCACATGGA GGAACTGTCTCCAAGGAACAGCTCTCCACCATTGAGCTGGACAGCTGCTCTGACCCAGGCTGCTGAGAAC GCCACCTCAGCTCTGTTGAGGGAGCAGGAAGGCTCACGTCCAGCTCCCCTCAGGCACAGATCTCCTGGCA ATGAAAGCGCCATCTCTCCAGCAAGCCGTGGAGATGCGGCTGAAGATCAGGTTCATAAGCTTCGGCTCAA ACTTCTTAAAAAATTAAAGGCAAAAAAGAAGAAACTAGCTGCCCTCATTTCTTCCCCGCACCGTGAACCA TCTCTAAGTGACCACTCAGAGCCTGCGTCCCATTGTGGAACACCTGCAAGTGACCAGTCAGAGCCTGTAT CCCATTGTGGATCTCCAAATGACTGTGAGTCCATAGAAGATCTGTTAAAAGAGCTACAGCATCAAATTGA CCTTGCAGACAGCAAATCTGGGTGCACCTCTGCTCCTGATGCCACCTCAAATAACAGCCAGAGTCATGAG GAGATTTTAGCAGAGTTGCTGTCGCCTACAGCCATGTCAGAGCCCTCAGAAAGTGGGGAGCTTGAGTTGA GGTACTTGGAAATGGGGGACAGCACCCCAGCACAAGCACCTAGTGAATTCAGTGTTGTCTCCCAGAACAC ATGTCTGAAACAGGACCATGATTACTGCAGCCCTGAGAAAGGCCAGCGGGAAGTTGATCTGCATTCAGTC ATGGACAGTGCCTGTATAAGAACCTTGAACTTGGGGAGTCCCATGAAGACTGATATCTTTGATGATTTTT TTTCCACCTCAGCACTGAATTCTTTAACAAACGACACATTAGACATACCTCATTTTGATGACTCTCTGTT TGAGAATTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231322 representing NM_001286825 Red=Cloning site Green=Tags(s) MPAKSRCLPLCQTLCVQWKNTQALCWLDCILSALVHLEVLRKTVLEACSREECVFGRLFEMYHQADELLH THHLHGVTGEDCKKLTSEIFTEIDTCLNKVRDEIFAKLQPKLRCTLGDMESPVFALPVLLKLEPHVESLF TYSFSWNFECSHCGHQYQNRCVKSLVTFTNIVPEWHPLNAAHFGPCNSCNSKSQIRKMVLERASPILMLH FVEGLPRRDLQHYAFHFEGSLYQVTSVIQYQANNHFITWFLDADGSWLECDDLKGPCAKRHVTCEVPASE THIVIWERKSQVPIEEAACLPCMKPNVQPVSGEEQPTCPALCSLAGTATSEPSVAHPTSMAGAPQTLPEI QAVAHGDSVLSGAKGMVDSILPSALEETIQETASVSQVDSKDCLLEDKPVAGSAALVRVLAFQPQDSPGS SGSSLVSSLCEGKLVAPCVDSSFPSQAVSTDLQAVLSQAGDTVVPNPVTDAPVPVLVQELKSLATEKDSQ TQLLPLKTEKLDPEQPGKSQASNLRKRETTASSKTVAARSAQNQPRKEDQKRAFVGSWVKGLLSRGGAFM PTCVLSQSRAVSDLQPSVKGASNFDGFKTKSISRRSKRMSRKAKHMEELSPRNSSPPLSWTAALTQAAEN ATSALLREQEGSRPAPLRHRSPGNESAISPASRGDAAEDQVHKLRLKLLKKLKAKKKKLAALISSPHREP SLSDHSEPASHCGTPASDQSEPVSHCGSPNDCESIEDLLKELQHQIDLADSKSGCTSAPDATSNNSQSHE EILAELLSPTAMSEPSESGELELRYLEMGDSTPAQAPSEFSVVSQNTCLKQDHDYCSPEKGQREVDLHSV MDSACIRTLNLGSPMKTDIFDDFFSTSALNSLTNDTLDIPHFDDSLFENC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286825
ORF Size	2670 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286825.1, NP_001273754.1
RefSeq Size	3714 bp
RefSeq ORF	2673 bp
Locus ID	231915
UniProt ID	Q3ULM6
MW	97.7 kDa
Gene Summary	SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function]

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