Acsl4 (NM_019477) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9430020A05Rik; ACS4; AU018108; Facl4; Lacs4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230841 representing NM_019477 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACT TCGACTCACTAGCTGTCATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTAGC CAAATTTGGGAAGAAGGACAGCCTTGGAACCCGGGAGATCCTGAGTGAAGAAAATGAAATGCAGCCAAAT GGAAAGGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATAAACTATCTTGAAGTGAACTGCAGAG TGAATAACTTTGGAAGTGGCCTCACTGCATTGGGACTGAAACCAAAGAACACCATTGCCATTTTCTGTGA GACCAGGGCAGAGTGGATGATTGCAGCACAGACTTGCTTTAAGTACAACTTTCCACTTGTGACTTTATAT GCCACACTTGGCAGAGAAGCTGTAGTTCATGGATTAAATGAATCTGAGGCTTCCTATCTGATTACTAGTG TTGAGCTTCTGGAAAGCAAACTGAAGGCGGCCTTAGTAGATATCAATTGTGTTAAACATATCATTTATGT GGATAATAAGACTATCAATAGAGCAGAGTACCCTGAGGGGCTTGAAATTCACAGCATGCAATCAGTAGAG GAGCTGGGAGCCAAGCCAGAAAACTTGAGCGTTCCTCCAAGTAGACCAACCCCTTCAGACATGGCCATTG TCATGTACACCAGTGGTTCTACGGGCCGCCCCAAGGGAGTGATGATGCATCATAGCAATTTGATTGCTGG AATGACAGGCCAGTGTGAACGTATCCCTGGACTAGGACCGAAGGACACATATATTGGCTACTTACCTTTG GCTCATGTGCTGGAACTGACAGCAGAGATATCATGCTTTACCTATGGCTGTAGGATTGGATACTCTTCAC CCCTTACACTGTCTGACCAGTCCAGCAAAATCAAGAAGGGAAGCAAGGGTGATTGTACTGTACTGAAACC CACACTTATGGCCGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAGGTTCAAGAG ATGAATTATGTTCAGAAAACTCTATTTAAAATCGGGTATGATTACAAATTAGAGCAAATCAAGAAAGGCT ATGACGCCCCTCTTTGTAATCTGATACTGTTTAAAAAGGTGAAGGCTCTGCTGGGAGGGAATGTCCGCAT GATGCTGTCTGGCGGCGCGCCACTGTCCCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCC ATTGGTCAGGGATATGGGCTGACAGAATCATGTGGTGCTGGAACAGTTACTGAAGTTACTGACTACACTA CTGGAAGAGTTGGAGCTCCTCTTATTTGCTGTGAAATTAAACTGAAAGACTGGCAGGAAGGTGGTTATAC AGTTCATGATAAGCCGAACCCCAGAGGTGAGATTGTGATCGGTGGCCAGAATATCTCCATGGGATATTTT AAAAACGAAGAGAAAACAGCAGAAGATTATTGTGTTGATGAAAATGGACAAAGGTGGTTTTGCACTGGCG ATATTGGAGAATTCCATCCTGATGGATGCTTACAGATTATAGATCGTAAGAAAGATCTGGTAAAGTTACA AGCAGGAGAATATGTATCTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTGATCGACAACATC TGTGCTTTTGCCAAAAGTGACCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAAGTTGACTC TTTTGGCACAACAGAAGGGGGTAGAAGGATCTTGGGTTGATATTTGCAATAATCCCGCCATGGAAGCTGA AATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCGATCAAGGTT CGGTTAAGCCCAGAGCCATGGACCCCCGAGACTGGTTTGGTAACAGATGCCTTCAAGCTGAAAAGGAAGG AGTTGAAGAACCATTATCTCAAAGACATTGAGCGAATGTATGGGGGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230841 representing NM_019477 Red=Cloning site Green=Tags(s) MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPN GKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLY ATLGREAVVHGLNESEASYLITSVELLESKLKAALVDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVE ELGAKPENLSVPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL AHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQE MNYVQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCP IGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYF KNEEKTAEDYCVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNI CAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKV RLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019477
ORF Size	2010 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019477.3, NP_062350.3
RefSeq Size	4990 bp
RefSeq ORF	2013 bp
Locus ID	50790
UniProt ID	Q9QUJ7
MW	74.8 kDa
Gene Summary	Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.[UniProtKB/Swiss-Prot Function]

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