Cnga3 (NM_001282010) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CNG3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230834 representing NM_001282010 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGGTGAACACCCAGTGTTCACAGCCCTCCCCGACCCAACTTTCAATAAAGAATGCGGACAGAG ATCTCGACCACGTAGAGAACGGCCTGGGCAGGTCTCACTCACCATGTGAGGAGACATCTTCCACACTGCA GCAAGGGATCGCCATGGAGACCCGAGGACTGGCTGGATCCGCACGGAGCGTAGTCACAAGCCAGGGACCA GCCAGGGTGTCACGCCTCATCATCTCGATTCGTGCGTGGGCCTCCAGGCACTTACACGATGAAGACCAGA CACCTGACTCCTTTTTGGATCGATTTCATGGATCTGAGCTTAAGGAAGTCTCCACCCGGGAAAGCAATGC CCAGCCCAACCCAGGAGAACAGAAGCCACCAGACGGAGGGGAAGGCAGGAAGGAGGAGCCCATTGTGGTG GACCCCTCCAGCAACATCTACTACCGCTGGCTGACTGCCATCGCGCTCCCAGTCTTCTATAACTGGTGTC TACTTGTATGCAGGGCCTGTTTTGATGAGCTACAATCAGAGCACCTGACGCTGTGGCTGGTCTTGGACTA CTCTGCAGATGTCCTTTATGTTTTAGACATGCTGGTTCGAGCCCGGACAGGTTTCCTTGAGCAAGGCCTA ATGGTCAGAGATACCAAGAGGCTGTGGAAACATTACACAAAGACCTTGCACTTCAAGCTGGACATCCTGT CTCTCATCCCCACAGACCTGGCTTATTTAAAGTTGGGCGTGAACTACCCAGAACTGAGGTTCAACCGCCT CCTGAAGTTCTCTCGGCTCTTTGAATTCTTTGACCGCACAGAGACAAGGACCAACTACCCCAACGTGTTC AGGATAGGGAACTTGGTCCTTTACACACTCATCATCATCCACTGGAATGCCTGCATCTACTTTGCCATTT CCAAGTTCATTGGTTTTGGGACAGACTCCTGGGTCTATCCAAACACCTCCAAGCCGGAGTATGCACGACT CTCCCGGAAGTACATTTACAGTCTCTACTGGTCCACCCTGACCCTGACCACCATTGGTGAGACCCCACCC CCCGTGAAAGATGAAGAGTATCTCTTTGTGGTCATCGACTTCCTGGTGGGTATCCTGATCTTTGCCACCA TAGTGGGCAACGTAGGCTCCATGATTTCCAACATGAACGCTCCCCGGGTAGAGTTCCAGGCTAAGATCGA CTCCGTCAAGCAGTACATGCAGTTCCGGAAGGTAACCAAGGACTTGGAGACTCGGGTTATCCGGTGGTTT GACTATCTGTGGGCCAACAGGAAGACGGTGGATGAAAAGGAAGTGCTCAAAAACCTCCCGGACAAGCTGA AGGCTGAGATCGCCATCAACGTGCACCTGGACACGCTGAAGAAGGTCCGAATCTTCCAGGACTGCGAGGC GGGGCTGCTGGTGGAGCTGGTGCTGAAGCTCCGCCCCACTGTGTTCAGCCCCGGGGACTACATATGCAAA AAGGGGGACATTGGAAGAGAGATGTACATCATCAAGGAGGGCAAGCTGGCTGTGGTGGCTGATGACGGGG TCACCCAGTTTGTGGTCCTCAGTGACGGCAGTTACTTTGGGGAGATTAGCATCTTAAACATTAAGGGGAG CAAGTCTGGGAACCGCAGGACGGCCAACATCAGGAGTATCGGCTACTCAGACCTGTTCTGCCTCTCCAAG GATGACCTAATGGAAGCCCTCACCGAGTACCCAGACGCTAAGAGGGCTCTGGAGGAAAAGGGCCGTCAGA TTCTGATGAAGGACAACCTAATTGATGAGGACCTAGTCGCGGCCAGGGTAGATACCAGGGACGTTGAGGA GAAGGTGGAGTACCTGGAGTCGTCCCTGGACATCCTGCAGACGAGGTTTGCCCGACTCCTGGCTGAGTAC AGTGCCTCCCAGATGAAGCTGAAACAGCGGCTCACTCGGCTGGAGAGCCAGATGAACAGGAGGTGTTGTG GCTTCTCACCTGACAGGGAGAATTCCGAGGATGCTTCAAAGACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230834 representing NM_001282010 Red=Cloning site Green=Tags(s) MAKVNTQCSQPSPTQLSIKNADRDLDHVENGLGRSHSPCEETSSTLQQGIAMETRGLAGSARSVVTSQGP ARVSRLIISIRAWASRHLHDEDQTPDSFLDRFHGSELKEVSTRESNAQPNPGEQKPPDGGEGRKEEPIVV DPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEHLTLWLVLDYSADVLYVLDMLVRARTGFLEQGL MVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGVNYPELRFNRLLKFSRLFEFFDRTETRTNYPNVF RIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPNTSKPEYARLSRKYIYSLYWSTLTLTTIGETPP PVKDEEYLFVVIDFLVGILIFATIVGNVGSMISNMNAPRVEFQAKIDSVKQYMQFRKVTKDLETRVIRWF DYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPTVFSPGDYICK KGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSK DDLMEALTEYPDAKRALEEKGRQILMKDNLIDEDLVAARVDTRDVEEKVEYLESSLDILQTRFARLLAEY SASQMKLKQRLTRLESQMNRRCCGFSPDRENSEDASKTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282010
ORF Size	2007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282010.1, NP_001268939.1
RefSeq Size	3623 bp
RefSeq ORF	2010 bp
Locus ID	12790
UniProt ID	Q9JJZ8
MW	76.6 kDa
Gene Summary	Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones (By similarity). Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3.[UniProtKB/Swiss-Prot Function]

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