Alas1 (NM_001291835) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ALAS; Alas-1; Alas-h; ALAS-N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230767 representing NM_001291835 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGTCGTTCGCCGATGCCCATTCTTATCCCGAGTCCCTCAGGCCTTTCTGCAGAAGGCAGGAA AGTCTCTGTTGTTCTATGCTCAAAACTGCCCCAAGATGATGGAAGTTGGGGCCAAGCCAGCTCCTCGAAC CCTGTCCACATCAGCTGTCCACTGCCAGCAGGTCAAAGAAACCCCTCCAGCCAATGAGAAAGAGAAAACT GCCAAGGCCGCAGTCCAGCAGGCTCCTGACGAGTCCCAGATGGCACAGACTCCAGACGGCACACAGCTTC CGTCTGGACACCCGTCCCCTGCTACAAGCCAGGGCTCTGGGAGCAAGTGCCCTTTCCTGGCAGCACAGCT GAGCCAGACAGGCAGCAGCGTCTTCCGCAAGGCCAGTCTGGAGCTCCAGGAGGACGTGCAGGAAATGCAT GCTGTGAGGAAAGAGGCTGCTCAAAGCCCAGTGCCTCCCAGCCTGGTTAATGTGAAAACCGATGGAGAGG ATCCAAGCCGACTGCTGAAGAACTTCCAGGACATCATGCGAAAGCAGAGGCCAGAAAGAGTGTCTCATCT TCTTCAGGATAACTTGCCAAAGTCTGTTTCCACTTTTCAATATGATCATTTCTTTGAGAAGAAAATTGAT GAGAAAAAAAATGATCATACCTACCGAGTTTTTAAAACGGTGAACCGGAGAGCACAAATCTTCCCGATGG CGGATGACTACACGGATTCCCTCATAACCAAAAAGCAGGTGTCGGTCTGGTGCAGTAATGACTACCTAGG CATGAGTCGACACCCACGGGTGTGTGGGGCTGTCATGGAGACTGTGAAACAGCATGGTGCTGGAGCTGGG GGAACTAGGAATATTTCTGGAACGAGCAAGTTCCACGTAGAACTGGAGCAGGCACTGGCCGACCTCCACG GCAAAGATGCGGCGCTCTTGTTCTCTTCCTGTTTTGTGGCCAACGACTCCACCCTCTTCACCCTGGCTAA GATGATGCCAGGCTGTGAAATTTACTCTGATTCCGGGAACCATGCCTCCATGATCCAAGGGATTCGCAAC AGTCGAGTGCCAAAATATATCTTCCGCCACAATGACGTTAACCATCTCAGAGAACTGCTGCAGAGATCTG ACCCCTCAGTCCCCAAGATAGTAGCATTTGAAACTGTCCATTCAATGGATGGAGCAGTGTGCCCACTGGA AGAGCTGTGTGACGTGGCCCATGAGTTTGGGGCAATCACGTTTGTGGATGAGGTCCATGCAGTGGGGCTC TATGGAGCTCGAGGTGGAGGGATCGGTGATCGGGATGGCGTCATGCCAAAAATGGACATCATCTCTGGGA CGCTTGGTAAAGCGTTCGGCTGTGTTGGAGGATACATTGCCAGCACGAGTTTGCTGATCGACACCGTCCG CTCCTACGCTGCGGGCTTCATCTTCACCACCTCCTTGCCACCAATGCTGCTGGCTGGAGCCCTGGAGTCT GTGCGGATCCTGAAGAGCAGCGAGGGGCGCGCCCTTCGCCGCCAGCACCAGCGAAATGTCAAGCTTCTGA GGCAGATGCTAATGGACGCTGGCCTCCCGGTCATCCACTGTCCGAGTCACATCATCCCTGTGCGGGTTGC CGATGCTGCTAAGAACACAGAGATCTGTGATGAGTTGATGACCAGGCATAATATCTACGTCCAGGCCATC AATTACCCAACAGTGCCTCGCGGGGAGGAGCTCCTGCGGATCGCCCCCACCCCGCATCACACCCCGCAGA TGATGAACTTCTTCGTGGAGAAGCTGCTGGTCACGTGGAAGCGAGTTGGGCTGGAACTTAAGCCACATTC GTCAGCTGAATGCAACTTCTGCAGGAGGCCCCTGCACTTTGAAGTGATGAGCGAAAGAGAGAAAGCCTAT TTCTCAGGCATGAGCAAGATGGTGTCTGCCCAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230767 representing NM_001291835 Red=Cloning site Green=Tags(s) METVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRTLSTSAVHCQQVKETPPANEKEKT AKAAVQQAPDESQMAQTPDGTQLPSGHPSPATSQGSGSKCPFLAAQLSQTGSSVFRKASLELQEDVQEMH AVRKEAAQSPVPPSLVNVKTDGEDPSRLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDHFFEKKID EKKNDHTYRVFKTVNRRAQIFPMADDYTDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMETVKQHGAGAG GTRNISGTSKFHVELEQALADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDSGNHASMIQGIRN SRVPKYIFRHNDVNHLRELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGL YGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSLLIDTVRSYAAGFIFTTSLPPMLLAGALES VRILKSSEGRALRRQHQRNVKLLRQMLMDAGLPVIHCPSHIIPVRVADAAKNTEICDELMTRHNIYVQAI NYPTVPRGEELLRIAPTPHHTPQMMNFFVEKLLVTWKRVGLELKPHSSAECNFCRRPLHFEVMSEREKAY FSGMSKMVSAQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291835
ORF Size	1926 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291835.1, NP_001278764.1
RefSeq Size	3089 bp
RefSeq ORF	1929 bp
Locus ID	11655
UniProt ID	Q8VC19
MW	71 kDa

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