Rbpj (NM_001277116) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI843960; CBF1; Igkjrb; Igkrsbp; RBP-J; RBP-J kappa; RBP-Jkappa; RBPjk; Rbpsuh
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230298 representing NM_001277116 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTTGCTGGGATTTGAACTCCTGACCTTCAGAAGAGCAGTCGGGTGCTCTTACCCATTGAGCCATC TCACCAGCCCGAAGTTTGGTGAGCGGCCTCCACCCAAACGACTCACTAGGGAAGCTATGCGAAATTATTT AAAAGAACGAGGGGATCAAACAGTGCTCATTCTTCATGCAAAAGTTGCACAGAAGTCTTACGGAAATGAA AAACGATTTTTTTGCCCTCCTCCTTGTGTGTATCTTATGGGCAGTGGTTGGAAGAAAAAAAAAGAGCAAA TGGAACGAGATGGATGTTCTGAACAAGAGTCTCAACCCTGTGCGTTTATTGGAATAGGAAACAGTGACCA AGAAATGCAGCAGCTGAACTTGGAAGGGAAGAACTACTGTACAGCCAAAACATTGTACATATCTGATTCA GACAAGAGAAAACATTTCATGTTGTCTGTAAAGATGTTCTATGGCAACAGCGATGACATTGGTGTGTTCC TCAGCAAGCGGATAAAGGTCATCTCCAAACCCTCCAAAAAGAAGCAGTCACTGAAGAATGCTGACTTGTG CATTGCTTCAGGAACGAAGGTGGCACTGTTCAATCGCCTTCGGTCCCAGACAGTTAGTACCAGGTACCTG CATGTAGAAGGAGGGAATTTCCACGCCAGTTCACAACAGTGGGGAGCATTTTACATCCATCTCTTGGACG ACGACGAGTCGGAAGGAGAGGAGTTCACAGTTAGAGATGGCTACATCCATTACGGGCAGACTGTCAAGCT TGTGTGCTCAGTGACTGGCATGGCACTCCCAAGATTGATAATTAGGAAAGTTGATAAGCAGACGGCATTA CTGGATGCAGACGACCCTGTATCACAACTCCACAAATGTGCATTTTACCTTAAGGATACAGAAAGAATGT ACTTGTGCCTTTCTCAAGAAAGAATAATCCAATTTCAGGCCACTCCATGTCCAAAAGAACAAAATAAGGA AATGATAAACGATGGAGCTTCCTGGACAATCATTAGCACAGACAAGGCCGAGTACACGTTCTATGAGGGA ATGGGCCCCGTCCTTGCCCCAGTCACCCCTGTGCCTGTCGTAGAAAGTCTTCAGTTGAATGGCGGCGGGG ACGTAGCAATGCTTGAACTTACAGGACAAAACTTTACTCCAAATTTAAGAGTGTGGTTTGGGGATGTAGA AGCCGAAACAATGTACAGATGTGGAGAGAGCATGCTCTGTGTGGTCCCAGACATTTCTGCATTCCGGGAA GGTTGGAGATGGGTCCGCCAGCCAGTCCAGGTTCCAGTGACTTTGGTCCGAAATGACGGGGTCATTTACT CCACCAGCCTTACCTTCACCTACACACCAGAGCCAGGGCCGAGGCCACACTGCAGCGCTGCAGGAGCGAT TCTCAGAGCCAACTCCAGCCAAGTGCCCTCCAATGAGTCAAACACAAACAGCGAGGGGAATTACACAAAT GCCAGCACAAATTCTACCAGTGTCACATCGTCCACAGCAACCGTGGTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230298 representing NM_001277116 Red=Cloning site Green=Tags(s) MWLLGFELLTFRRAVGCSYPLSHLTSPKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNE KRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDS DKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYL HVEGGNFHASSQQWGAFYIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTAL LDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEQNKEMINDGASWTIISTDKAEYTFYEG MGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFRE GWRWVRQPVQVPVTLVRNDGVIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPSNESNTNSEGNYTN ASTNSTSVTSSTATVVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277116
ORF Size	1521 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277116.1, NP_001264045.1
RefSeq Size	5590 bp
RefSeq ORF	1524 bp
Locus ID	19664
MW	57.2 kDa
Gene Summary	Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations (PubMed:7566092). Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (PubMed:26194095).[UniProtKB/Swiss-Prot Function]

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