Dclre1c (NM_001302674) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9930121L06Rik; A; AI661365; Art; Snm1l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230085 representing NM_001302674 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTTCGGGTGAGGAGACTTCAGACTGGCAAAAGGAGAAGCTTCCAGAATGGAGCTTCTGCACTCT GGAGAGTAAAAGACATCCAAAGTGTGTATTTAGACACGACTTTCTGTGACCCAAGGTTTTATCAGATCCC AAGTCGTGAGCAGTGTTTGAGGGGCATTTTGGAGCTGGTTCGGAGCTGGGTCACTAGGAGTCCGCACCAC GTCGTGTGGCTGAACTGTAAAGCAGCTTATGGCTACGAGTATTTATTCACCAACCTAAGCGAGGAGCTGG GAGTTCAGGTTCATGTGGACAAGCTGGACATGTTTAAAAACATGCCTGATATCCTGCACCATCTCACAAC GGACAGAAACACCCAGATCCACGCCTGCCGCCACCCAAAGGCAGAAGAGTGTTTTCAGTGGAATAAACTA CCCTGTGGTATAACTTCCCAAAACAAAACTGCACTCCACACAATCAGCATCAAGCCATCTACCATGTGGT TTGGAGAGAGGACCAGAAAAACCAACGTGATCGTTAGGACTGGAGAGAGCTCATACAGAGCTTGCTTCTC TTTTCACTCCTCCTTCAGTGAGATTAAAGATTTTTTGAGCTACATCTGCCCAGTGAATGTGTATCCAAAT GTCATCCCAGTTGGCCTCACTGTGGATAAGGTCATGGACGTTTTAAAGCCTCTGTGCCGGTCTCCCCAAA GTGTTGAGCCAAAGTACAAACCGCTTGGAAAATTGAAGAGAGCCAGAACAATCCATCTTGACTCGGAGGA AGACGATGATCTCTTTGATGACCCTCTACCGACACCTTTAAGGCACAAAGTTCCGTACCAGCTAACTCTT CAGCCTGAGCTGTTTTCAATGAAGGCACTGCCACTAGACCAGCCTGAACTGAGACAAAGCCCAGGAGGCT GCAAAGCAGAGAGTGTGTGGAGCCCTTCTTTGGCTAACTTCATAGACTGTGAAGAATCCAACAGCGACAG TGGAGAAGAGCTAGAAACCCCACCACCCTCACTGCAGGGAGGTCTGGGCCCCTCGACACTCGTCCAGCAA AATGCTGATCCGGATGTGGACATACCACAGTGGGAAGTCTTCTTCAAAAGAAGAGATGAAATCACAGTGG ACACCATGATTAGAACTCCCAGACCCAGAAAGATGAAAGGCTGTGGTCAATGGAGTCTGAAGATGCTTCT CCAGAACCTGGAAATACAGGAAGAGAAACATATATTTGAGAACAGGGGCTGGAAGATGGCTGGGCAGGTA AAGGGTAGCTGTGGTCTTTTGGAGGGCCAGAGTTCACTCCCAACATTCAAGCTAGCCACCTCCCTAGCCT CAAACTCCAGCTTCTGGCCTCCATGGCACCTGCATTCACATGCGCACACACAAATCTTTACCTTTAAAAA AAAAACCAAAACTTTACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230085 representing NM_001302674 Red=Cloning site Green=Tags(s) MRLRVRRLQTGKRRSFQNGASALWRVKDIQSVYLDTTFCDPRFYQIPSREQCLRGILELVRSWVTRSPHH VVWLNCKAAYGYEYLFTNLSEELGVQVHVDKLDMFKNMPDILHHLTTDRNTQIHACRHPKAEECFQWNKL PCGITSQNKTALHTISIKPSTMWFGERTRKTNVIVRTGESSYRACFSFHSSFSEIKDFLSYICPVNVYPN VIPVGLTVDKVMDVLKPLCRSPQSVEPKYKPLGKLKRARTIHLDSEEDDDLFDDPLPTPLRHKVPYQLTL QPELFSMKALPLDQPELRQSPGGCKAESVWSPSLANFIDCEESNSDSGEELETPPPSLQGGLGPSTLVQQ NADPDVDIPQWEVFFKRRDEITVDTMIRTPRPRKMKGCGQWSLKMLLQNLEIQEEKHIFENRGWKMAGQV KGSCGLLEGQSSLPTFKLATSLASNSSFWPPWHLHSHAHTQIFTFKKKTKTLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302674
ORF Size	1419 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302674.1, NP_001289603.1
RefSeq Size	3896 bp
RefSeq ORF	1422 bp
Locus ID	227525
UniProt ID	Q8K4J0
MW	54.7 kDa
Gene Summary	This gene encodes a member of the SNM1 family of nucleases and is involved in V(D)J recombination and DNA repair. This protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Homozygous knockout mice for this gene exhibit severe combined immunodeficiency with sensitivity to ionizing radiation. Mutations in this gene in humans can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

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