Calcoco2 (NM_001271018) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2410154J16Rik; C77254; Ndp52; Ndp52l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229288 representing NM_001271018, codon optimized. Due to the complexity of NM_002856, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGTGTCCAATCCCAACCCTGCTGGAACATGGCAATTTTTCCCAGGTACTTTTTAACAACGTTG AAAAATTCTACGCACCACGGGGGGACATCATGTGTTACTATACCCTGACAGAGAAGTTCATCCCAAGAAG GAAAGATTGGATCGGGATCTTTAAAGTTGGGTGGAAGACAACCCAGGAGTACTATACCTTCATGTGGGCC CCCCTGCCAAAGGACCAGAACAAAGACTCCGCAACCCAGCAGGAGATCCAATTCAAGGCATACTATCTCC CAAAGGACGTGGAACGATACCAGTTCTGTTACGTGGACGAGGACGGGCTTGTGAGAGGAACATCTGTGCC ATTTCAGTTCTGTCCAGATCCCGATGAAGACATAATGGTGGTGATTAACAAGGAGAAAGTTGAAGAGATG GAACAGCTTAGTGAGGAATTGTACCAACAGAATCAGGAACTGAAAGACAAGTACGCAGATCTGCACGAGC AGCTGCAACGCAAGCAAGTGGCATTGGAGGCTACTCAGCGGGTGAACAAAACGCTCGAACACAAGGTGGA AGAGAAGGCCTCTTGGGAGAAGGAAAAGGCTAGCTGGGAGGAAGAGAAAGCAAGTTGGGAAGAGGAAAAA GCTTCCTGGGAGGAGGAGAAAGCCAGCTGGGAGGAGGAAAAAGCCTCTTGGGAAGAGGAGAAAGCGAGCT GGGAAGAGGAAAAGGCCTCCTGGGAAGAGGAGAAGGCGTCCTGGGAGGAAGAGAAGGCCTCTTGGGAGGA GGAGAAGGCCTCCTGGGAAGAAGAGAAGGCCTCATGGGAAGAGGAGAAGGCCTCTTGGGAGGAAGAGAAG GCGTCATGGGAAGAGGAAAAGGCATCCTGGGAGAAAGAAAAAGCAAGCTGGGAGGAAGAGAAGGCTTCCT GGGAGAAAGAGAAGGCCCCCTGGGAAGTTGAGAAGGCCCCCTGGAAGGAAGTGAAGGCATATTGGTGGAA TGACCTTCATCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229288 representing NM_001271018 Red=Cloning site Green=Tags(s) MDQCPIPTLLEHGNFSQVLFNNVEKFYAPRGDIMCYYTLTEKFIPRRKDWIGIFKVGWKTTQEYYTFMWA PLPKDQNKDSATQQEIQFKAYYLPKDVERYQFCYVDEDGLVRGTSVPFQFCPDPDEDIMVVINKEKVEEM EQLSEELYQQNQELKDKYADLHEQLQRKQVALEATQRVNKTLEHKVEEKASWEKEKASWEEEKASWEEEK ASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEKASWEEEK ASWEEEKASWEKEKASWEEEKASWEKEKAPWEVEKAPWKEVKAYWWNDLHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271018
ORF Size	993 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	2029 bp
RefSeq ORF	996 bp
Locus ID	76815
UniProt ID	A2A6M5
MW	40.1 kDa
Gene Summary	Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation (By similarity). Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (By similarity). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (By similarity). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (By similarity). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (By similarity).[UniProtKB/Swiss-Prot Function]

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