Ppt2 (NM_001302396) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	0610007M19Rik; AA672937
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229116 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGCTATGGAGGCAGAGGCTTCCTTCGGCTTGGGCTTTGCTTCTCCTGCCGTTCCTGCCGCTGC TGATGCCCGCAGCCCCCGCAGCCCACCGCGGGTCCTACAAGCCCGTGATCGTGGTGCACGGGCTCTTTGA CAGTTCATACAGCTTCCGCCACCTGCTGGACTATATCAATGAGACACACACCGGGACTGTGGTGACAGTG CTTGATCTCTTCGATGGCAGAGAGAGTTTGCGGCCCCTGTGGGAACAGGTACAAGGGTTCCGAGAGGCTG TGGTCCCCATCATGGAAAAGGCCCCTGAAGGAGTGCACCTCATCTGCTACTCCCAGGGGGGCCTGGTGTG CCGTGCTTTGCTGTCTGTCATGGATAACCACAATGTGGACTCTTTCATCTCCCTCTCCTCCCCACAGATG GGCCAGTATGGAGACACGGACTATTTGAAATGGCTCTTCCCCACGTCCATGCGGTCTAACCTCTATCGGG TCTGCTATAGTCCTTGGGGCCAGGAATTTTCCATTTGCAACTACTGGCACGATCCTCACCACGATGACTT GTACCTCAATGCCAGCAGCTTTCTGGCCCTCATCAATGGGGAAAGAGACCATCCCAATGCCACTGCATGG CGGAAGAACTTCCTTCGCGTGGGCCGTCTGGTGCTGATTGGGGGTCCTGATGATGGCGTTATCACTCCCT GGCAATCTAGCTTCTTTGGTTTCTATGATGCCAATGAGACAGTTCTGGAGATGGAGGAGCAGCCGGTGTA TCTTCGAGATTCTTTTGGGTTGAAGACTCTCCTGGCCCGGGGGGCCATAGTGAGGTGTCCCATGGCTGGC ATCTCTCACACCACGTGGCACTCTAACCGTACGCTCTACGATACTTGCATTGAGCCGTGGCTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229116 protein sequence Red=Cloning site Green=Tags(s) MPGLWRQRLPSAWALLLLPFLPLLMPAAPAAHRGSYKPVIVVHGLFDSSYSFRHLLDYINETHTGTVVTV LDLFDGRESLRPLWEQVQGFREAVVPIMEKAPEGVHLICYSQGGLVCRALLSVMDNHNVDSFISLSSPQM GQYGDTDYLKWLFPTSMRSNLYRVCYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATAW RKNFLRVGRLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQPVYLRDSFGLKTLLARGAIVRCPMAG ISHTTWHSNRTLYDTCIEPWLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302396
ORF Size	909 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302396.1, NP_001289325.1
RefSeq Size	1710 bp
RefSeq ORF	909 bp
Locus ID	54397
UniProt ID	O35448
MW	34.4 kDa
Gene Summary	Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins (By similarity).[UniProtKB/Swiss-Prot Function]

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