Galt (NM_001302511) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW553376
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228486 representing NM_001302511 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGCTGTTCTAACCCCCATCCCCACTGCCAGGTTTGGGCTAGCAGCTTCCTGCCAGATATCGCCC AGCGTGAAGAGCGATCCCAGCAGACCTATCACAGCCAGCATGGAAAACCTTTGTTATTGGAATATGGTCA CCAAGAGCTCCTCAGGAAGGAACGTCTGGTCCTAACCAGTGAGCACTGGATAGTTCTGGTCCCCTTCTGG GCAGTGTGGCCTTTCCAGACACTTCTGCTGCCCCGGCGGCACGTGCGGCGGCTACCTGAGCTGAACCCCG CTGAGCGTGATGATCTCGCCTCCATCATGAAGAAGCTCTTGACCAAGTACGACAATCTATTTGAGACATC CTTTCCCTACTCCATGGGCTGGCATGGGGCTCCCACGGGATTAAAGACTGGAGCCACCTGTGACCACTGG CAGCTCCACGCCCACTACTACCCCCCACTTCTGCGATCCGCAACTGTCCGGAAGTTCATGGTTGGCTATG AAATGCTTGCCCAGGCCCAGCGTGACCTCACTCCCGAACAGGCCGCAGAAAGATTAAGGGCGCTTCCCGA GGTACACTATTGCCTGGCGCAGAAAGACAAGGAAACGGCAGCCATTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228486 representing NM_001302511 Red=Cloning site Green=Tags(s) MMGCSNPHPHCQVWASSFLPDIAQREERSQQTYHSQHGKPLLLEYGHQELLRKERLVLTSEHWIVLVPFW AVWPFQTLLLPRRHVRRLPELNPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGLKTGATCDHW QLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYCLAQKDKETAAIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302511
ORF Size	609 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302511.2
RefSeq Size	1544 bp
RefSeq ORF	612 bp
Locus ID	14430
MW	24 kDa
Gene Summary	The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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