United States
Germany
Japan
United Kingdom
China
Tgfb1 (NM_011577) Mouse Tagged ORF Clone
CAT＃: MR227339
- TrueORF®
Tgfb1 (Myc-DDK-tagged) - Mouse transforming growth factor, beta 1 (Tgfb1)
ORF Plasmid: tGFP
"NM_011577" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
| Cited in 9 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TGF-beta; TGF-beta1; Tgfb; Tgfb-1; TGFbeta; TGFbeta1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227339 representing NM_011577
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCCTCGGGGCTGCGGCTACTGCCGCTTCTGCTCCCACTCCCGTGGCTTCTAGTGCTGACGCCCG GGAGGCCAGCCGCGGGACTCTCCACCTGCAAGACCATCGACATGGAGCTGGTGAAACGGAAGCGCATCGA AGCCATCCGTGGCCAGATCCTGTCCAAACTAAGGCTCGCCAGTCCCCCAAGCCAGGGGGAGGTACCGCCC GGCCCGCTGCCCGAGGCGGTGCTCGCTTTGTACAACAGCACCCGCGACCGGGTGGCAGGCGAGAGCGCCG ACCCAGAGCCGGAGCCCGAAGCGGACTACTATGCTAAAGAGGTCACCCGCGTGCTAATGGTGGACCGCAA CAACGCCATCTATGAGAAAACCAAAGACATCTCACACAGTATATATATGTTCTTCAATACGTCAGACATT CGGGAAGCAGTGCCCGAACCCCCATTGCTGTCCCGTGCAGAGCTGCGCTTGCAGAGATTAAAATCAAGTG TGGAGCAACATGTGGAACTCTACCAGAAATATAGCAACAATTCCTGGCGTTACCTTGGTAACCGGCTGCT GACCCCCACTGATACGCCTGAGTGGCTGTCTTTTGACGTCACTGGAGTTGTACGGCAGTGGCTGAACCAA GGAGACGGAATACAGGGCTTTCGATTCAGCGCTCACTGCTCTTGTGACAGCAAAGATAACAAACTCCACG TGGAAATCAACGGGATCAGCCCCAAACGTCGGGGCGACCTGGGCACCATCCATGACATGAACCGGCCCTT CCTGCTCCTCATGGCCACCCCCCTGGAAAGGGCCCAGCACCTGCACAGCTCACGGCACCGGAGAGCCCTG GATACCAACTATTGCTTCAGCTCCACAGAGAAGAACTGCTGTGTGCGGCAGCTGTACATTGACTTTAGGA AGGACCTGGGTTGGAAGTGGATCCACGAGCCCAAGGGCTACCATGCCAACTTCTGTCTGGGACCCTGCCC CTATATTTGGAGCCTGGACACACAGTACAGCAAGGTCCTTGCCCTCTACAACCAACACAACCCGGGCGCT TCGGCGTCACCGTGCTGCGTGCCGCAGGCTTTGGAGCCACTGCCCATCGTCTACTACGTGGGTCGCAAGC CCAAGGTGGAGCAGTTGTCCAACATGATTGTGCGCTCCTGCAAGTGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227339 representing NM_011577
Red=Cloning site Green=Tags(s) MPPSGLRLLPLLLPLPWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPP GPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDI REAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQ GDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRAL DTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGA SASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011577 |
| ORF Size | 1170 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011577.2 |
| RefSeq Size | 2094 bp |
| RefSeq ORF | 1173 bp |
| Locus ID | 21803 |
| UniProt ID | P04202 |
| MW | 44.8 kDa |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. Mice lacking a functional copy of this gene develop severe multifocal inflammatory disease, yolk sac defects and colon cancer. [provided by RefSeq, Aug 2016] |
Citations (9)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ursodeoxycholic acid reduces antitumor immunosuppression by inducing CHIP-mediated TGF-β degradation
,null,
Nature Communications
,PubMed ID 35701426
[Tgfb1]
|
|
Tracking of an Oral Salmonella-Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice
,Mbongue, JC;Alhoshani, A;Rawson, J;Garcia, PA;Gonzalez, N;Ferreri, K;Kandeel, F;Husseiny, MI;,
Front Immunol
,PubMed ID 32411136
[TGFB1]
|
|
Comprehensive Molecular Profiles of Functionally Effective Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles in Immunomodulation
,Kim, H;Lee, M;Bae, E;Ryu, J;Kaur, G;Kim, H;Kim, J;Barreda, H;Jung, S;Choi, J;Shigemoto-Kuroda, T;Oh, J;Lee, R;,
Molecular Therapy
,PubMed ID 32380062
[TGFB1]
|
|
Factors affecting Salmonella-based combination immunotherapy for prevention of type 1 diabetes in non-obese diabetic mice
,Husseiny, MI;Du, W;Mbongue, J;Lenz, A;Rawson, J;Kandeel, F;Ferreri, K;,
Vaccine
,PubMed ID 30416020
[TGFB1]
|
|
Lower-Molecular-Weight Chitosan-Treated Polyethyleneimine: a Practical Strategy For Gene Delivery to Mesenchymal Stem Cells
,Liu, M;Zhang, L;Zhao, Q;Jiang, X;Wu, L;Hu, Y;,
Cell. Physiol. Biochem.
,PubMed ID 30355922
[TGFB1]
|
|
A Milieu Molecule for TGF-β Required for Microglia Function in the Nervous System
,Qin, Y;Garrison, BS;Ma, W;Wang, R;Jiang, A;Li, J;Mistry, M;Bronson, RT;Santoro, D;Franco, C;Robinton, DA;Stevens, B;Rossi, DJ;Lu, C;Springer, TA;,
Cell
,PubMed ID 29909984
[TGFB1]
|
|
Proteomic Analysis of NRROS Interactome Reveals the Presence of Chaperones and Mediators of the ERAD Pathway
,Noubade, R;Phung, Q;Phung, W;Verschueren, E;Lau, L;Konno, H;Lill, J;Ouyang, W;,
bioRxiv
[TGFB1]
|
|
Overexpression of Latent TGFß Binding Protein 4 in Muscle Ameliorates Muscular Dystrophy through Myostatin and TGFß
,Lamar, KM;Bogdanovich, S;Gardner, BB;Gao, QQ;Miller, T;Earley, JU;Hadhazy, M;Vo, AH;Wren, L;Molkentin, JD;McNally, EM;,
PLoS Genet.
,PubMed ID 27148972
[Tgfb1]
|
|
An oral vaccine for type 1 diabetes based on live attenuated Salmonella
,Husseiny, MI;Rawson, J;Kaye, A;Nair, I;Todorov, I;Hensel, M;Kandeel, F;Ferreri, K;,
Vaccine
,PubMed ID 24631074
[TGFB1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227339 | Tgfb1 (tGFP-tagged) - Mouse transforming growth factor beta 1 (Tgfb1), (10ug) |
CNY 4,370.00 |
|
| MR227339L1 | Lenti ORF clone of Tgfb1 (Myc-DDK-tagged) - Mouse transforming growth factor, beta 1 (Tgfb1) |
CNY 5,890.00 |
|
| MR227339L2 | Lenti ORF clone of Tgfb1 (mGFP-tagged) - Mouse transforming growth factor, beta 1 (Tgfb1) |
CNY 5,890.00 |
|
| MR227339L3 | Lenti ORF clone of Tgfb1 (Myc-DDK-tagged) - Mouse transforming growth factor, beta 1 (Tgfb1) |
CNY 5,890.00 |
|
| MR227339L4 | Lenti ORF clone of Tgfb1 (mGFP-tagged) - Mouse transforming growth factor, beta 1 (Tgfb1) |
CNY 5,890.00 |
