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Sphk1 (NM_011451) Mouse Tagged ORF Clone
CAT＃: MR227335
- TrueORF®
Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1
ORF Plasmid: tGFP
"NM_011451" in other vectors (6)
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CNY 5,856.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110006G24Rik; Sk1; Spk1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227335 representing NM_011451
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTGGTGTTGTGTTTTGTTTGTAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGC TGGTGCTGCTGAACCCCCAGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTT CCTGGAGGAGGCAGAGATAACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTG TGTGCAGAGGAGTTGGGTCACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGG TGAATGGGCTAATGGAACGGCCAGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGG CTCCGGCAATGCGCTGGCAGCTTCTGTGAACCACTATGCTGGGTACGAGCAGGTGACTAATGAAGACCTG CTCATCAACTGCACACTGCTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTG CTTCTGGGCTGCGGCTCTATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAG TGAGAAGTACAGGCGCTTGGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGC ATCTACCAAGGCCAACTGGCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGG TGCAGAAGGGCCCCGTCGACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGT ACCAGAACAGGACTTCGTCCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCA CCCATGGGCCGCTGTGAGGCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGC TGCTGCGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGT GCCCGTGGTTGCTTTCCGCCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATG GTATGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCC CATCCGGCCGGGACTCCCGGCGGGGGCCACCTCCAGAAGAACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227335 representing NM_011451
Red=Cloning site Green=Tags(s) MWWCCVLFVVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELV CAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDL LINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLR IYQGQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAA PMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELM VCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011451 |
| ORF Size | 1164 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011451.3, NP_035581.1 |
| RefSeq Size | 1537 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 20698 |
| UniProt ID | Q8CI15 |
| MW | 43.7 kDa |
| Gene Summary | This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209438 | Sphk1 (untagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG227335 | Sphk1 (tGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1) transcript variant 1, (10ug) |
CNY 4,370.00 |
|
| MR227335L1 | Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1 |
CNY 5,890.00 |
|
| MR227335L2 | Lenti ORF clone of Sphk1 (mGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1 |
CNY 8,256.00 |
|
| MR227335L3 | Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1 |
CNY 5,890.00 |
|
| MR227335L4 | Lenti ORF clone of Sphk1 (mGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1 |
CNY 8,256.00 |
