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Runx2 (NM_001146038) Mouse Tagged ORF Clone
CAT＃: MR227321
- TrueORF®
Runx2 (Myc-DDK-tagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 1
ORF Plasmid: tGFP
"NM_001146038" in other vectors (4)
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CNY 5,896.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AM; AML3; Cbf; Cbfa; Cbfa-1; Cbfa1; LS3; Os; Osf2; PEB; Pebp2a1; PEBP2aA; Pebpa2a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227321 representing NM_001146038
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCAAACAGCCTCTTCAGCGCAGTGACACCGTGTCAGCAAAGCTTCTTTTGGGATCCGAGCACCA GCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCAAGATGAGCGACGTGAGCCCGGTGGTGGC TGCGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAACAGCAACAGCAACAACAG CAGCAGCAGCAGCAGCAGCAGGAGGCGGCCGCAGCAGCAGCGGCGGCAGCGGCGGCGGCAGCAGCGGCGG CGGCCGCAGTGCCCCGATTGAGGCCGCCGCACGACAACCGCACCATGGTGGAGATCATCGCGGACCACCC GGCCGAACTGGTCCGCACCGACAGTCCCAACTTCCTGTGCTCCGTGCTGCCCTCGCACTGGCGGTGCAAC AAGACCCTGCCCGTGGCCTTCAAGGTTGTAGCCCTCGGAGAGGTACCAGATGGGACTGTGGTTACCGTCA TGGCCGGGAATGATGAGAACTACTCCGCCGAGCTCCGAAATGCCTCCGCTGTTATGAAAAACCAAGTAGC CAGGTTCAACGATCTGAGATTTGTGGGCCGGAGCGGACGAGGCAAGAGTTTCACCTTGACCATAACAGTC TTCACAAATCCTCCCCAAGTGGCCACTTACCACAGAGCTATTAAAGTGACAGTGGACGGTCCCCGGGAAC CAAGAAGGCACAGACAGAAGCTTGATGACTCTAAACCTAGTTTGTTCTCTGATCGCCTCAGTGATTTAGG GCGCATTCCTCATCCCAGTATGAGAGTAGGTGTCCCGCCTCAGAACCCACGGCCCTCCCTGAACTCTGCA CCAAGTCCTTTTAATCCACAAGGACAGAGTCAGATTACAGATCCCAGGCAGGCACAGTCTTCCCCACCGT GGTCCTATGACCAGTCTTACCCCTCCTATCTGAGCCAGATGACATCCCCATCCATCCACTCCACCACGCC GCTGTCTTCCACACGGGGCACCGGGCTACCTGCCATCACTGACGTGCCCAGGCGTATTTCAGATGATGAC ACTGCCACCTCTGACTTCTGCCTCTGGCCTTCCTCTCTCAGTAAGAAGAGCCAGGCAGGTGCTTCAGAAC TGGGCCCTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCATCCCTCACTGAGAGCCGCTTCTCCAACCC ACGAATGCACTACCCAGCCACCTTTACCTACACCCCGCCAGTCACGTCAGGCATGTCCCTCGGCATGTCC GCCACCACTCACTACCACACGTACCTGCCACCACCCTACCCCGGCTCTTCCCAAAGCCAGAGTGGACCCT TCCAGACCAGCAGCACTCCATATCTCTACTATGGTACTTCGTCAGCATCCTATCAGTTCCCAATGGTACC CGGGGGAGACCGGTCTCCTTCCAGGATGGTCCCACCATGCACCACCACCTCGAATGGCAGCACGCTATTA AATCCAAATTTGCCTAACCAGAATGATGGTGTTGACGCTGACGGAAGCCACAGCAGTTCCCCAACTGTTT TGAATTCTAGCGGCAGAATGGATGAGTCTGTTTGGCGGCCATAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227321 representing NM_001146038
Red=Cloning site Green=Tags(s) MASNSLFSAVTPCQQSFFWDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQ QQQQQQQEAAAAAAAAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCN KTLPVAFKVVALGEVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITV FTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSA PSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDD TATSDFCLWPSSLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMS ATTHYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLL NPNLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146038 |
| ORF Size | 1584 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146038.2, NP_001139510.1 |
| RefSeq Size | 5898 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 12393 |
| UniProt ID | Q08775 |
| MW | 57.9 kDa |
| Gene Summary | This gene encodes a member of the runt domain-containing family of transcription factors. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Sep 2015] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors
,null,
eLife
,PubMed ID 36861967
[Runx2]
|
|
Co-occupancy identifies transcription factor co-operation for axon growth
,Venkatesh, I;Mehra, V;Wang, Z;Simpson, MT;Eastwood, E;Chakraborty, A;Beine, Z;Gross, D;Cabahug, M;Olson, G;Blackmore, MG;,
Nature communications
,PubMed ID 33953205
[RUNX2]
|
|
Direct Reprogramming of Mouse Fibroblasts into Functional Osteoblasts
,Zhu, H;Swami, S;Yang, P;Shapiro, F;Wu, JY;,
J. Bone Miner. Res.
,PubMed ID 31793059
[RUNX2]
|
|
Characterization of Runx2 Phosphorylation Sites Required for TGF-β1-Mediated stimulation of Matrix Metalloproteinase-13 Expression in Osteoblastic cells
,Arumugam, B;Vairamani, M;Partridge, NC;Selvamurugan, N;,
J. Cell. Physiol.
,PubMed ID 28419442
[RUNX2]
|
|
SIRT6 deficiency culminates in low-turnover osteopenia
,Sugatani, T;Agapova, O;Malluche, HH;Hruska, KA;,
Bone
,PubMed ID 26189760
[RUNX2]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217412 | Runx2 (untagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 1, (10ug) |
CNY 5,904.00 |
|
| MG227321 | Runx2 (tGFP-tagged) - Mouse runt related transcription factor 2 (Runx2) transcript variant 1, (10ug) |
CNY 5,040.00 |
|
| MR227321L3 | Lenti ORF clone of Runx2 (Myc-DDK-tagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 1 |
CNY 8,296.00 |
|
| MR227321L4 | Lenti ORF clone of Runx2 (mGFP-tagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 1 |
CNY 8,296.00 |
