Pcdh15 (NM_001142742) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227209 representing NM_001142742 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA GCTGGGGCCAGTATGACGATGACTGGCAATACGAGGATTGCAAACTAGCTAGGGGAGGACCACCAGCTAC TATCGTGGCCATTGATGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACT GCCGGAGGACCAGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACC CCGTTAAACAGATGCTTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACA CTCCATTGTGGTGCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATC GTGGTGCGAGATCGGAATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGC TCACTCCAGTTGGCACCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGG CCCTAATGGACAGATAGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAA ATTCCACTCATGCTGACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACT ATGTCATCATCCAAGCAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCAC AGTAGATGTTCTAGATGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGT GACTGTCGTCCACTCACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTT TGGTGACACCACCTATCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCAT CCTCTACTCCATCCTTGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCA GAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGC AGGACAATGGCCACCCACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAG TCCATACTTCACAATGCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCT GAGAGCCTAAACTTAACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACGTGCCACCTG GTGGAGTTCCTACAAAAGATCCAGAGCTCCACCTCTTCCTGAATGACTACACCTCGGTCTTCACTGTGAC ACCCACTGGTATCACCCGCTACCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTACACC TTTCTGATAACAGCGTTTGATGGCGTGCAAGAAAGTGAGCCAGTCGTGGTCAATATCCGAGTGATGGATG CAAATGATAACACGCCCACCTTCCCTGAAATCTCCTATGATGTCTATGTTTACACAGACATGAGTCCTGG GGACAGCGTCATTCAGCTGACAGCGGTAGATGCTGATGAAGGCTCTAATGGGGAGATCTCCTATGAAATA CTGGTGGGGGGCAAGGGAGACTTCGTGATCAACAAGACCACAGGGCTGGTGAGCATTGCACCAGGCGTGG AGCTGATCGTGGGACAGACGTATGCGCTCACAGTGCAGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAG GCACTCCATCTGCACAGTGTACATCGAGGTGCTTCCTCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAG CTGATGTACAGTCTGGAAGTCAGCGAGGCCATGAGGATCGGTGCTATTTTATTAAATCTACAGGCAACTG ATCGAGAGGGAGATCCAATCACATATGCCATCGAGAATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGA AACCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGACCGCGAGAGCACAGACCGCTACATCCTCATCGTC ACAGCCTCAGATGGCAGACCGGATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACGGACGTCAATG ACAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTT TGTGGGTCAAGTCCGGGCAACAGACCCAGATGCTGGGATAAACGGCCAAGTTCACTACAGCCTGGGGAAC TTCAACAACCTCTTCCGCATCACATCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCA GGGACCACTATGAACTGGTTGTCGTGGCAACAGATGGAGCAGTCCACCCTCGACATTCAACTCTGACACT GTACATCAAGGTGTTGGACATTGATGATAACAGTCCTGTTTTTACCAATTCAACGTACACAGTTGTCGTT GAAGAGAATCTGCCAGCCGGGACCTCCTTTCTTCAAATAGAGGCCAAGGATGTTGACCTTGGAGCCAATG TGTCATATCGGATCAGAAGCCCAGAAGTGAAACACCTTTTTGCACTGCATCCATTCACTGGAGAATTGTC TCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCGGACCAGGAGGCAAGCATCACATTCTTGGTGGAGGCC TTTGACATTTATGGGACTATGCCACCTGGTATAGCAACAGTCACGGTAATTGTGAAGGACATGAATGACT ACCCTCCAGTGTTTAGCAAACGCATCTACAAGGGGATGGTGGCTCCAGATGCAGTCAAGGGGACACCAAT CACCACCGTTTATGCTGAAGATGCGGACCCACCTGGGATGCCTGCAAGTAGGGTGAGGTATCGAGTGGAC GACGTGCAGTTTCCATACCCAGCCAGTATTTTTGATGTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCG TCAATCTTAATGAAGAGCCTACTACGATTTTCAAGCTGGTGGTTGTGGCTTTTGATGACGGCGAACCTGT GATGTCCAGCAGTGCCACGGTGAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAG GAATACAGACCTCCTCCTGTAAGTGAGCTTGCGGCCAGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTG CCATTAATCAGAGCATCGTGTACTCCATTGTGGCAGGAAATGAGGAAGACAAGTTTGGAATCAACAATGT CACTGGGGTCATCTATGTGAATTCACCATTGGATTACGAGACAAGGACCAGCTATGTGCTCCGGGTACAA GCAGATTCTCTGGAAGTGGTCCTTGCCAATCTCCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACA TTGAGATTCAGGATGAAAACGATCACCCCCCAGTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGA AGACGCAAGGATGTTCGCATCTGTGCTCAGAGTGAAGGCCACCGACAGGGACACGGGTAATTACAGTGCC ATGGCCTACCGGCTCATCATACCGCCGATTAAAGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACAG GTCTCATCAAGACAGCCATGCTCTTCCACAATATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAAC TGACGACTACGGGAAGGGGTTGAGCGGGAAAGCAGACGTACTGGTCTCCGTGGTCAATCAACTGGATATG CAGGTCATTGTCTCCAATGTGCCCCCTACACTAGTGGAAAAGAAGATAGAAGACCTTACAGAGATTTTGG ATCGCTACGTTCAGGAGCAAATTCCTGGTGCCAAGGTTGTGGTGGAGTCCATAGGTGCCCGTCGCCATGG AGACGCCTACTCCCTAGAAGACTATAGCAAGTGCGACCTGACTGTCTATGCCATCGACCCGCAGACCAAC AGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTGGACGGCAAACTGCTCGATATCAATAAAGACTTCC AGCCGTATTACGGGGAAGGAGGGCGCATTCTGGAGATTCGGACACCTGAGGCAGTGACGAGCATCAAGAA GCGAGGAGAAAGCTTGGGGTACACAGAAGGGGCCTTGCTGGCCTTGGCCTTCATCATCATCCTCTGTTGC ATCCCAGCCATCTTGGTCGTCTTAGTAAGCTACCGACAGTTTAAAGTACGCCAGGCTGAGTGCACGAAGA CCGCAAGAATTCAGTCTGCTATGCCTGCAGCCAAGCCTGCAGCTCCTGTACCAGCTGCGCCTGCGCCGCC CCCGCCCCCGCCACCACCACCACCAGGAGCACATCTCTATGAAGAACTGGGAGAGAGCGCAATGCATAAG TATGAGATGCCCCAGTATGGAAGTCGCCGTCGACTGCTGCCACCTGCTGGACAGGAGGAATACGGCGAAG TCATTGGTGAAGCTGAAGAGGAATATGAAGAAGAAGAGGTAGAGCCAGAGAAAGTTAAAAAACCCAAAGT TGAAATTAGAGAGCCTAGTGAGGAGGAGGTGGTAGTCACCGTTGAGAAGCCACCAGCGGCTGAGCCCACA TACCCAACGTGGAAGAGAGCCAGGATATTCCCGATGATTTTTAAGAAAGTCAGAGGTCTCGCTGAGAAAA GAGGCATTGACCTTGAGGGCGAGGAGTGGAGGAGGCGCCTTGATGAAGAAGACAAAGACTATCTTCAACT GACTCTAGACCAGGAGGAAGCTACCGAAAGCACCGTGGAGTCAGAGGAGGAGTCCAGCGACTACACAGAA TACACAGAAACGGAGTCCGAGTTCAGCGAGTCCGAGACAACTGAAGAATCAGAGTCGGAGACCCCATCTG AGGAAGCGGAGGAGAGCTCTACCCCGGAGTCAGAGGAGTCTGAGTCCACTGAGTCAGAGGGAGAGAAAGC AAGAAAAAACATCGTGCTAGCTAGAAGAAGGCCTGTGGTCGAGGAAATCCAGGAGGTGAAAGGTAAGAGA GAGGAGCCCCCGGTGGAAGAGGAAGAAGAGCCCCCACTAGAGGAGGAAGAACGGGCAGAGGAAGGAGAAG AAAGCGAAGCAGCTCCCATGGATGAGTCCACAGACCTGGAGGCTCAGGATGTCCCAGAGGAGGGCAGTGC AGAATCAGTCTCCATGGAGAGGGGCGTGGAAAGTGAGGAGTCAGAGTCAGAACTGAGCAGCAGCAGCAGT ACCAGTGAGAGTCTCTCCGGAGGCCCCTGGGGCTTTCAGGTGCCAGAATATGACAGAAGGAAGGATGAAG AGCCCAAGAAATCTCCAGGCGCAAACTCCGAAGGTTACAACACAGCCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227209 representing NM_001142742 Red=Cloning site Green=Tags(s) MFLQFAVWKCLPHGILIASLLVVSWGQYDDDWQYEDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGT AGGPDPTIELSLKDNVDYWVLLDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCVNKKVGTVIYHEVRI VVRDRNDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPEDPTSNDTFE IPLMLTGNVVLRKRLNYEDKTRYYVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTR DCRPLTYQAAIPELRTPEELNPILVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTA ELTLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFASLHIEILDENNQSPYFTMPSYQGYILESAPVGATIS ESLNLTTPLRIVALDKDIEDVPPGGVPTKDPELHLFLNDYTSVFTVTPTGITRYLTLLQPVDREEQQTYT FLITAFDGVQESEPVVVNIRVMDANDNTPTFPEISYDVYVYTDMSPGDSVIQLTAVDADEGSNGEISYEI LVGGKGDFVINKTTGLVSIAPGVELIVGQTYALTVQASDNAPPAERRHSICTVYIEVLPPNNQSPPRFPQ LMYSLEVSEAMRIGAILLNLQATDREGDPITYAIENGDPQRVFNLSETTGILSLGKALDRESTDRYILIV TASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVGQVRATDPDAGINGQVHYSLGN FNNLFRITSNGSIYTAVKLNREARDHYELVVVATDGAVHPRHSTLTLYIKVLDIDDNSPVFTNSTYTVVV EENLPAGTSFLQIEAKDVDLGANVSYRIRSPEVKHLFALHPFTGELSLLRSLDYEAFPDQEASITFLVEA FDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITTVYAEDADPPGMPASRVRYRVD DVQFPYPASIFDVEEDSGRVVTRVNLNEEPTTIFKLVVVAFDDGEPVMSSSATVRILVLHPGEIPRFTQE EYRPPPVSELAARGTVVGVISAAAINQSIVYSIVAGNEEDKFGINNVTGVIYVNSPLDYETRTSYVLRVQ ADSLEVVLANLRVPSKSNTAKVYIEIQDENDHPPVFQKKFYIGGVSEDARMFASVLRVKATDRDTGNYSA MAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDM QVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDAYSLEDYSKCDLTVYAIDPQTN RAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCC IPAILVVLVSYRQFKVRQAECTKTARIQSAMPAAKPAAPVPAAPAPPPPPPPPPPGAHLYEELGESAMHK YEMPQYGSRRRLLPPAGQEEYGEVIGEAEEEYEEEEVEPEKVKKPKVEIREPSEEEVVVTVEKPPAAEPT YPTWKRARIFPMIFKKVRGLAEKRGIDLEGEEWRRRLDEEDKDYLQLTLDQEEATESTVESEEESSDYTE YTETESEFSESETTEESESETPSEEAEESSTPESEESESTESEGEKARKNIVLARRRPVVEEIQEVKGKR EEPPVEEEEEPPLEEEERAEEGEESEAAPMDESTDLEAQDVPEEGSAESVSMERGVESEESESELSSSSS TSESLSGGPWGFQVPEYDRRKDEEPKKSPGANSEGYNTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001142742
ORF Size	5370 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001142742.1, NP_001136214.1
RefSeq Size	6303 bp
RefSeq ORF	5373 bp
Locus ID	11994
UniProt ID	Q99PJ1
MW	199.4 kDa
Gene Summary	Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function]

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