Pcdh15 (NM_001142741) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227204 representing NM_001142741 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA GCTGGGGCCAGTATGACGATGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACTGCCGGAGGACC AGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACCCCGTTAAACAG ATGCTTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACACTCCATTGTGG TGCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATCGTGGTGCGAGA TCGGAATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGCTCACTCCAGTT GGCACCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGGCCCTAATGGAC AGATAGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAAATTCCACTCAT GCTGACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACTATGTCATCATC CAAGCAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCACAGTAGATGTTC TAGATGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGTGACTGTCGTCC ACTCACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTTTGGTGACACCA CCTATCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCATCCTCTACTCCA TCCTTGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCAGAACTCACTCT CCTGGAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGCAGGACAATGGC CACCCACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAGTCCATACTTCA CAATGCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCTGAGAGCCTAAA CTTAACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACACAAAAGATCCAGAGCTCCAC CTCTTCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACTGGTATCACCCGCTACCTCACCCTGC TTCAACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGATAACAGCGTTTGATGGCGTGCAAGA AAGTGAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGATAACACGCCCACCTTCCCTGAAATC TCCTATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGCGTCATTCAGCTGACAGCGGTAGATG CTGATGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGGGGGGCAAGGGAGACTTCGTGATCAA CAAGACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGATCGTGGGACAGACGTATGCGCTCACA GTGCAGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGGCACTCCATCTGCACAGTGTACATCGAGGTGC TTCCTCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAGCTGATGTACAGTCTGGAAGTCAGCGAGGCCAT GAGGATCGGTGCTATTTTATTAAATCTACAGGCAACTGATCGAGAGGGAGATCCAATCACATATGCCATC GAGAATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGAAACCACAGGGATTCTCAGCCTAGGGAAGGCTC TAGACCGCGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCTCAGATGGCAGACCGGATGGAACCTC AACTGCCACTGTGAACATAGTGGTGACGGACGTCAATGACAACGCTCCCGTGTTCGATCCCTATCTGCCC AGGAACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGTCAAGTCCGGGCAACAGACCCAGATG CTGGGATAAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACAACCTCTTCCGCATCACATCCAACGG GAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCACTATGAACTGGTTGTCGTGGCAACA GATGGAGCAGTCCACCCTCGACATTCAACTCTGACACTGTACATCAAGGTGTTGGACATTGATGATAACA GTCCTGTTTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGAATCTGCCAGCCGGGACCTCCTTTCT TCAAATAGAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATATCGGATCAGAAGCCCAGAAGTGAAA CACCTTTTTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTGAGGAGTTTGGATTATGAGGCCTTTC CGGACCAGGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACATTTATGGGACTATGCCACCTGGTAT AGCAACAGTCACGGTAATTGTGAAGGACATGAATGACTACCCTCCAGTGTTTAGCAAACGCATCTACAAG GGGATGGTGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACCGTTTATGCTGAAGATGCGGACCCAC CTGGGATGCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGCAGTTTCCATACCCAGCCAGTATTTT TGATGTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCTTAATGAAGAGCCTACTACGATTTTC AAGCTGGTGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCCAGCAGTGCCACGGTGAGAATTCTTG TCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACAGACCTCCTCCTGTAAGTGAGCTTGC GGCCAGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAATCAGAGCATCGTGTACTCCATTGTG GCAGGAAATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGGGTCATCTATGTGAATTCACCATTGG ATTACGAGACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATTCTCTGGAAGTGGTCCTTGCCAATCT CCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACATTGAGATTCAGGATGAAAACGATCACCCCCCA GTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGAAGACGCAAGGATGTTCGCATCTGTGCTCAGAG TGAAGGCCACCGACAGGGACACGGGTAATTACAGTGCCATGGCCTACCGGCTCATCATACCGCCGATTAA AGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACAGGTCTCATCAAGACAGCCATGCTCTTCCACAAT ATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAACTGACGACTACGGGAAGGGGTTGAGCGGGAAAG CAGACGTACTGGTCTCCGTGGTCAATCAACTGGATATGCAGGTCATTGTCTCCAATGTGCCCCCTACACT AGTGGAAAAGAAGATAGAAGACCTTACAGAGATTTTGGATCGCTACGTTCAGGAGCAAATTCCTGGTGCC AAGGTTGTGGTGGAGTCCATAGGTGCCCGTCGCCATGGAGACGCCTACTCCCTAGAAGACTATAGCAAGT GCGACCTGACTGTCTATGCCATCGACCCGCAGACCAACAGAGCCATCGACAGAAATGAGCTTTTTAAGTT CCTGGACGGCAAACTGCTCGATATCAATAAAGACTTCCAGCCGTATTACGGGGAAGGAGGGCGCATTCTG GAGATTCGGACACCTGAGGCAGTGACGAGCATCAAGAAGCGAGGAGAAAGCTTGGGGTACACAGAAGGGG CCTTGCTGGCCTTGGCCTTCATCATCATCCTCTGTTGCATCCCAGCCATCTTGGTCGTCTTAGTAAGCTA CCGACAACGCCAGGCTGAGTGCACGAAGACCGCAAGAATTCAGTCTGCTATGCCTGCAGCCAAGCCTGCA GCTCCTGTACCAGCTGCGCCTGCGCCGCCCCCGCCCCCGCCACCACCACCACCAGGAGCACATCTCTATG AAGAACTGGGAGAGAGCGCAATGCATAATCTTTTCCTTCTCTACCATTTTGAACAAAGCAGGGGAAATAA CTCAGTCCCAGAAGACAGGAGCAGTCATCGCGATGGGATGGCCTTTTCCTCCAGTACCACTGAGTCTCAT GAGCCAGCTCATGTAGAGGGACCACTTAAGGAGAGCCAGCCTAACCCAGCAAGGACGTTCTCATTTGTTC CTGATGAGGATAACTTAAGTACCCATAATCCCCTTTACATGGAAAGTATAGGTCAAAGGTCAACAAACTC AGACCTTCAGCCACGAACAGATTTTGAAGAGCTGTTGGCACCCAGAACACAAGTTAAGAGTCAGTCTCTG AGGGGCCCAAGAGAAAAGATCCAGAGGGTGTGGAATCAGTCTGTGAGCTTTCCTAGGCGGCTCATGTGGA AAGCCCCAAACAGGCCAGAGACCATAGACCTGGTGGAGTGGCAGATCACCAATCAGAGAGCTGAATGCGA AAGCGCCAGATGCCACCCAAGCCAGAGAGGTAGCAGCAACGTTCTGCTGGCAACTGAAGATGCCCACGAG TCAGAGAAAGAAGGGGGACACAGAGACACCCTAATCGTCCAGCAAACAGAGCAGCTGAAATCTCTGTCTT CTGGCTCTTCTTTTTCCTCCTCTTGGTCTCACTTTTCTTTCTCAACTCTGCCAACGATTTCCAGAGCGGT GGAACTCGGGTCGGAACCTAATGTGGTCACTTCTCCCGCTGACTGCACCTTGGAACTTTCTCCTCCTCTG AGACCCCGTATTTTAAACTCCTTAAGCTCTAAGAGAGAGACTCCCACATGTGCATCAGATACAGAACCAA AAAGGAACTCTTTTGAGATCGCTCCCCATCCACCTAGCATCTCTGCTCCCCTCCCACATCCGCCTCTTCC TAGACCTCCCATTGCCTTTACCACTTTTCCTCTTCCCCTTTCTCCCCCTAACCCTCCTCCCCCACAACTT GTTACATTTTCTCTTCCCATTTCTACACCCCCTACTTCTTCTCTACCTCTTCCTCCTCCACTGTCACTTC CTCCTCCTCCTCGGCCACCAGCTCCCCGCCTCTTCCCACAGCCTCCTTCCACGTCCATTCCATCCACAGA CAGCATCTCTGCACCAGCTGCTAAATGCACTGCCAGTGCCACACACGCCAGAGAAACCACGTCTACGACA CAGCCACCAGCATCCAACCCGCAGTGGGGGGCAGAACCCCACAGACATCCAAAAGGGATCCTCAGACATG TGAAAAACTTGGCAGAGCTCGAGAAATCAGTGTCTAACATGTACAGTCACATAGAAAAAAACTGCCCACC TGCAGATCCCTCAAAACTACACACGTTTTGCCCTGCAGAGAAAACAGGCATGAAAATCACACATGACCAG AGCCAGGAAACGTTGGTTAGAGTTGTTGAGGGAATTGACGTGCAACCTCACAGTCAATCAACATCTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227204 representing NM_001142741 Red=Cloning site Green=Tags(s) MFLQFAVWKCLPHGILIASLLVVSWGQYDDGTILVDNMLIKGTAGGPDPTIELSLKDNVDYWVLLDPVKQ MLFLNSTGRVLDRDPPMNIHSIVVQVQCVNKKVGTVIYHEVRIVVRDRNDNSPTFKHESYYATVNELTPV GTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPEDPTSNDTFEIPLMLTGNVVLRKRLNYEDKTRYYVII QANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLTYQAAIPELRTPEELNPILVTP PIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTAELTLLEPVNRDFHQKFDLVIKAEQDNG HPLPAFASLHIEILDENNQSPYFTMPSYQGYILESAPVGATISESLNLTTPLRIVALDKDIEDTKDPELH LFLNDYTSVFTVTPTGITRYLTLLQPVDREEQQTYTFLITAFDGVQESEPVVVNIRVMDANDNTPTFPEI SYDVYVYTDMSPGDSVIQLTAVDADEGSNGEISYEILVGGKGDFVINKTTGLVSIAPGVELIVGQTYALT VQASDNAPPAERRHSICTVYIEVLPPNNQSPPRFPQLMYSLEVSEAMRIGAILLNLQATDREGDPITYAI ENGDPQRVFNLSETTGILSLGKALDRESTDRYILIVTASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLP RNLSVVEEEANAFVGQVRATDPDAGINGQVHYSLGNFNNLFRITSNGSIYTAVKLNREARDHYELVVVAT DGAVHPRHSTLTLYIKVLDIDDNSPVFTNSTYTVVVEENLPAGTSFLQIEAKDVDLGANVSYRIRSPEVK HLFALHPFTGELSLLRSLDYEAFPDQEASITFLVEAFDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYK GMVAPDAVKGTPITTVYAEDADPPGMPASRVRYRVDDVQFPYPASIFDVEEDSGRVVTRVNLNEEPTTIF KLVVVAFDDGEPVMSSSATVRILVLHPGEIPRFTQEEYRPPPVSELAARGTVVGVISAAAINQSIVYSIV AGNEEDKFGINNVTGVIYVNSPLDYETRTSYVLRVQADSLEVVLANLRVPSKSNTAKVYIEIQDENDHPP VFQKKFYIGGVSEDARMFASVLRVKATDRDTGNYSAMAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHN MRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDMQVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGA KVVVESIGARRHGDAYSLEDYSKCDLTVYAIDPQTNRAIDRNELFKFLDGKLLDINKDFQPYYGEGGRIL EIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCCIPAILVVLVSYRQRQAECTKTARIQSAMPAAKPA APVPAAPAPPPPPPPPPPGAHLYEELGESAMHNLFLLYHFEQSRGNNSVPEDRSSHRDGMAFSSSTTESH EPAHVEGPLKESQPNPARTFSFVPDEDNLSTHNPLYMESIGQRSTNSDLQPRTDFEELLAPRTQVKSQSL RGPREKIQRVWNQSVSFPRRLMWKAPNRPETIDLVEWQITNQRAECESARCHPSQRGSSNVLLATEDAHE SEKEGGHRDTLIVQQTEQLKSLSSGSSFSSSWSHFSFSTLPTISRAVELGSEPNVVTSPADCTLELSPPL RPRILNSLSSKRETPTCASDTEPKRNSFEIAPHPPSISAPLPHPPLPRPPIAFTTFPLPLSPPNPPPPQL VTFSLPISTPPTSSLPLPPPLSLPPPPRPPAPRLFPQPPSTSIPSTDSISAPAAKCTASATHARETTSTT QPPASNPQWGAEPHRHPKGILRHVKNLAELEKSVSNMYSHIEKNCPPADPSKLHTFCPAEKTGMKITHDQ SQETLVRVVEGIDVQPHSQSTSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001142741
ORF Size	5739 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001142741.1, NP_001136213.1
RefSeq Size	8974 bp
RefSeq ORF	5742 bp
Locus ID	11994
UniProt ID	Q99PJ1
MW	211.8 kDa
Gene Summary	Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function]

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