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Disc1 (NM_174853) Mouse Tagged ORF Clone
CAT＃: MR227173
- TrueORF®
Disc1 (Myc-DDK-tagged) - Mouse disrupted in schizophrenia 1 (Disc1), transcript variant 2
ORF Plasmid: tGFP
"NM_174853" in other vectors (4)
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CNY 7,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227173 representing NM_174853
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGCGGGGGTCCCCGGGGCGCTCCGATCCACAGTCCGAGCCACGGCGCAGACAGTGGGCATGGCT TACCGCCTGCAGTAGCCCCTCAGAGGCGGCGGCTGACACGGAGACCAGGCTACATGAGAAGCACAGCGGG TTCTGGGATCGGGTTCCTCTCTCCAGCAGTGGGCATGCCACACCCGAGCTCAGCAGGGCTGACAGGCCAG CAGTCCCAACACTCACAGTCCAAGGCTGGACAGTGCGGACTTGACCCTGGGAGCCACTGCCAAGCCTCAC TGGTGGGCAAGCCTTTTCTCAAGAGCTCCCTTGTCCCTGCTGTGGCCTCTGAGGGCCACCTGCACCCAGC CCAGCGCTCTATGAGAAAAAGACCAGTGCACTTTGCGGTTCATTCCAAGAATGACAGCAGACAATCTGAG AGGCTGACTGGGTCATTTAAGCCTGGGGACAGTGGGTTTTGGCAAGAATTATTATCTTCAGACAGCTTTA AGTCTCTGGCTCCTAGCCTTGATGCACCCTGGAACAAGGGATCAAGGGGCCTGAAGACTGTGAAACCTCT GGCATCACCGGCGTTGAATGGCCCCGCTGATATCGCATCCCTTCCCGGCTTCCAAGACACCTTCACTTCC AGCTTCAGCTTCATCCAACTCTCCCTTGGTGCTGCTGGAGAACGCGGAGAAGCAGAAGGTTGCCTGCCAT CCAGAGAGGCCGAACCTCTGCATCAGAGGCCCCAAGAGATGGCAGCTGAAGCATCTAGCTCAGACAGGCC CCACGGGGATCCTCGGCATCTCTGGACCTTCAGTCTTCACGCTGCTCCAGGCTTGGCGGACTTGGCTCAG GTGACAAGGAGCAGCAGCAGGCAATCAGAATGTGGCACGGTCTCCTCCTCCTCCTCGGACACTGGCTTCT CTTCCCAGGATGCATCCTCCGCTGGTGGGCGGGGCGACCAGGGCGGCGGCTGGGCCGATGCCCATGGATG GCATACATTGCTCAGGGAATGGGAGCCCATGCTGCAGGACTACCTACTGAGCAACCGCAGGCAGCTGGAG GTCACTTCCTTAATTTTAAAGCTTCAGAAATGTCAAGAAAAAGTGGTCGAGGATGGCGATTACGATACTG CAGAGACATTGAGACAGAGGTTGGAAGAACTGGAACAGGAGAAAGGCCGCCTGTCCTGGGCTCTGCCTTC ACAGCAACCTGCTCTTCGCAGCTTCTTGGGTTACCTGGCAGCACAGATACAGGTGGCCTTGCATGGAGCC ACCCAAAGGGCCGGCAGCGATGATCCAGAAGCCCCACTTGAAGGACAGCTGAGGACTACCGCCCAGGATA GCCTGCCTGCATCCATCACCAGGAGGGACTGGCTTATTCGAGAGAAACAGCGATTGCAGAAGGAAATCGA AGCTCTCCAAGCACGGATGTCTGCGCTGGAGGCAAAGGAAAAACGGCTGAGCCAAGAGTTGGAGGAGCAG GAGGTGCTGCTCCGGTGGCCGGGCTGTGACCTGATGGCACTGGTGGCCCAGATGTCCCCAGGCCAGCTGC AGGAGGTCAGCAAGGCCTTGGGAGAGACCCTGACCTCTGCCAACCAGGCTCCCTTCCAGGTGGAGCCACC TGAGACCCTCAGGAGCCTCCGGGAAAGGACAAAATCACTGAACCTGGCTGTCAGAGAACTCACTGCTCAG GTGTGCTCAGGTGAGAAGCTGTGCAGCTCTCTGAGGAGGAGACTCAGTGACCTCGACACCAGGCTGCCTG CCTTGCTGGAAGCCAAGATGCTGGCCCTATCAGAAACACGCATGAAGGCAAACACTGTGAAGTGCATGGA AGTGTTGGAAGGTCAGCTGAGCAGCTGCAGGTGCCCGCTGCTTGGGAGAGTGTGGAAAGCAGACTTGGAG ACTTGTCAGTTGCTAATGCAGAGCCTGCAGCTTCAGGAAGCAGGCAGCAGCCCACACGCAGAGGACGAGG AGCAGGTGCATAGCACAGGAGAGGCCGCCCAGACAGCTGCTCTGGCTGTCCCTCGAACACCCCACCCCGA AGAAGAAAAGTCCCCCTTGCAGGTGCTCCAGGAGTGGGACACCCACTCAGCTCTTTCACCACACTGTGCT GCAGGCCCATGGAAAGAGGATTCTCACATCGTTTCTGCTGAAGTTGGAGAAAAGTGCGAAGCCATAGGCG TGAAGCTCCTACACCTGGAAGACCAGCTTCTCGGAGCCATGTACAGTCACGATGAGGCTCTCTTTCAGTC TCTCCAGGGGGAGCTCCAGACGGTGAAGGAAACACTGCAGGCCATGATCCTGCAGCTCCAGCCAACAAAG GAGGCAGGAGAGGCCTCAGCTTCCTATCCGACAGCTGGTGCTCAGGAAACCGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227173 representing NM_174853
Red=Cloning site Green=Tags(s) MQGGGPRGAPIHSPSHGADSGHGLPPAVAPQRRRLTRRPGYMRSTAGSGIGFLSPAVGMPHPSSAGLTGQ QSQHSQSKAGQCGLDPGSHCQASLVGKPFLKSSLVPAVASEGHLHPAQRSMRKRPVHFAVHSKNDSRQSE RLTGSFKPGDSGFWQELLSSDSFKSLAPSLDAPWNKGSRGLKTVKPLASPALNGPADIASLPGFQDTFTS SFSFIQLSLGAAGERGEAEGCLPSREAEPLHQRPQEMAAEASSSDRPHGDPRHLWTFSLHAAPGLADLAQ VTRSSSRQSECGTVSSSSSDTGFSSQDASSAGGRGDQGGGWADAHGWHTLLREWEPMLQDYLLSNRRQLE VTSLILKLQKCQEKVVEDGDYDTAETLRQRLEELEQEKGRLSWALPSQQPALRSFLGYLAAQIQVALHGA TQRAGSDDPEAPLEGQLRTTAQDSLPASITRRDWLIREKQRLQKEIEALQARMSALEAKEKRLSQELEEQ EVLLRWPGCDLMALVAQMSPGQLQEVSKALGETLTSANQAPFQVEPPETLRSLRERTKSLNLAVRELTAQ VCSGEKLCSSLRRRLSDLDTRLPALLEAKMLALSETRMKANTVKCMEVLEGQLSSCRCPLLGRVWKADLE TCQLLMQSLQLQEAGSSPHAEDEEQVHSTGEAAQTAALAVPRTPHPEEEKSPLQVLQEWDTHSALSPHCA AGPWKEDSHIVSAEVGEKCEAIGVKLLHLEDQLLGAMYSHDEALFQSLQGELQTVKETLQAMILQLQPTK EAGEASASYPTAGAQETEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174853 |
| ORF Size | 2367 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174853.2, NP_777278.2 |
| RefSeq Size | 2408 bp |
| RefSeq ORF | 2370 bp |
| Locus ID | 244667 |
| UniProt ID | Q811T9 |
| MW | 85.9 kDa |
| Gene Summary | Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221665 | Disc1 (untagged) - Mouse disrupted in schizophrenia 1 (Disc1), transcript variant 2, (10ug) |
CNY 10,170.00 |
|
| MG227173 | Disc1 (tGFP-tagged) - Mouse disrupted in schizophrenia 1 (Disc1) transcript variant 2, (10ug) |
CNY 8,650.00 |
|
| MR227173L3 | Lenti ORF clone of Disc1 (Myc-DDK-tagged) - Mouse disrupted in schizophrenia 1 (Disc1), transcript variant 2 |
CNY 9,790.00 |
|
| MR227173L4 | Lenti ORF clone of Disc1 (mGFP-tagged) - Mouse disrupted in schizophrenia 1 (Disc1), transcript variant 2 |
CNY 9,790.00 |
